Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds

Abstract

This study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. In vitro, proliferation (colony-forming rate) and migration (scratch) assays using cultured primary ovine keratinocytes were performed with or without 0.025% and 0.08% Omega-7, respectively. The colony-forming rate of keratinocytes in the Omega-7 group at 72 and 96 h were significantly higher than in the control (P < 0.05). The percentage of closure in scratch assay in the Omega-7 group was significantly higher than in the control at 17 h (P < 0.05). In vivo, efficacy of 4% Omega-7 isolated from buckthorn oil was assessed at 7 and 14 days in grafted ovine burn and donor site wounds. Telomerase activity, keratinocyte growth factor, and wound nitrotyrosine levels were measured at day 14. Grafted sites: Un-epithelialized raw surface area was significantly lower and blood flow was significantly higher in the Omega-7-treated sites than in control sites at 7 and 14 days (P < 0.05). Telomerase activity and levels of keratinocyte growth factors were significantly higher in the Omega-7-treated sites after 14 days compared to those of control (P < 0.05). The wound 3-nitrotyrosine levels were significantly reduced by Omega-7. Donor sites: the complete epithelialization time was significantly shorter and blood flow at day 7 was significantly higher in the Omega-7-treated sites compared to control sites (P < 0.05). In summary, topical application of Omega-7 accelerates healing of both grafted burn and donor site wounds. Omega-7 should be considered as a cost-efficient and effective supplement therapy for burn wound healing.

To compare the effect of sea buckthorn and silver sulfadiazine dressing on period of wound healing in patients with second‐degree burns: A randomized triple‐blind clinical trial

“…Studies have shown that sea buckthorn contains compounds such as omega-3, omega-6, and tocopherols, which facilitate the healing process by reducing wound inflammation. 13,18 In the present program, the size of second-degree burns was reduced during the treatment period in two groups treated with either sea buckthorn cream or 1% SSD cream, but the recovery of wound breadth was faster in the sea buckthorn group. Chandra et al in their study maintained that Sea buckthorn contains a high level of essential fatty acids which in addition to nourishing the human's skin tissue, can accelerate the process of treatment of burns.…”

“…Goudarzi et al 43 in their study reported that in addition to its anti‐inflammatory properties, sea buckthorn facilitates the wound healing process due to its analgesic and anti‐hypersensitivity effects, moreover. Studies have shown that sea buckthorn contains compounds such as omega‐3, omega‐6, and tocopherols, which facilitate the healing process by reducing wound inflammation 13,18 …”

“…13,14 Laboratory research, furthermore, has shown that sea buckthorn contains different elements, such as vitamins, minerals and fatty acids that, according to studies, are effective in the wound healing process. 15,16 While extensive research has been conducted into identifying the efficacy of sea buckthorn in wound healing in non-human samples, [17][18][19] rare research programs examining the effect of sea buckthorn in burns of human samples have been carried out. 20 Thus, considering SSD is the most common and the main medicine used in routine treatment of burn wounds in our country Iran, 21 researchers of the present study designed this controlled clinical trial aiming at evaluating the period of wound healing with sea buckthorn topical cream dressings for the second-degree burns and comparing the results with the 1% SSD dressings.…”

Modulation of oxidative and nitrosative stress attenuates microvascular hyperpermeability in ovine model of Pseudomonas aeruginosa sepsis

“…Levels of lung tissue 3-nitrotyrosine (06–284; MilliporeSigma, MA) and VEGF-A (ab46154; Abcam, MA), which is a major mediator of microvascular hyperpermeability 33 – 35 , were determined by automated capillary Western blot analysis (Wes™ ; ProteinSimple, San Jose, CA), as previously described 24 , 36 .…”

A Fatty Acid Fraction Purified From Sea Buckthorn Seed Oil Has Regenerative Properties on Normal Skin Cells

“…Although they are important components of the sea buckthorn, fatty acids (FA) have been less investigated in terms of biological effects (for a recent review on their impact on human health, see (Solà Marsiñach and Cuenca, 2019). It is known that sea buckthorn oil is rich in saturated and unsaturated FA and has an unusually high concentration of palmitoleic acid with proven benefits on skin health (Weimann et al, 2018;Niimi et al, 2021). Little is known about the implication of other FAs in cellular regeneration, given the fact that they are a major component of cell membrane lipids.…”