Omega-3 production by fish oil hydrolysis using a lipase from Burkholderia gladioli BRM58833 immobilized and stabilized by post-immobilization techniques

Martins, Pedro Alves; Trobo-Maseda, Lara; Lima, Frederico Alves; Júnior, Wilson Galvão de Morais; Marco, Janice Lisboa De; Salum, T. F. C.; Guisán, José M.

doi:10.1016/j.bbrep.2021.101193

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Despite substantial efforts in protein engineering aimed at modifying industrial enzyme catalysts, a gap persists between advancements at the laboratory level and large-scale production. In recent years, significant contributions to the field have been made by immobilization techniques for lipid-modifying enzymes (Ahrari et al 2022 ; Akil et al 2020 ; Enespa et al 2022 ; Martins et al 2022 ; Pacheco et al 2022 ; Verdasco-Martín et al 2018 ; Zhang et al 2022a ). Free enzymes show low operational stability, have high costs, and cannot be easily recovered or reused at the end of the reaction, hindering product separation (Almeida et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Enzyme engineering for functional lipids synthesis: recent advance and perspective

Guan,

Hou,

Yang

et al. 2024

Bioresour. Bioprocess.

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Functional lipids, primarily derived through the modification of natural lipids by various processes, are widely acknowledged for their potential to impart health benefits. In contrast to chemical methods for lipid modification, enzymatic catalysis offers distinct advantages, including high selectivity, mild operating conditions, and reduced byproduct formation. Nevertheless, enzymes face challenges in industrial applications, such as low activity, stability, and undesired selectivity. To address these challenges, protein engineering techniques have been implemented to enhance enzyme performance in functional lipid synthesis. This article aims to review recent advances in protein engineering, encompassing approaches from directed evolution to rational design, with the goal of improving the properties of lipid-modifying enzymes. Furthermore, the article explores the future prospects and challenges associated with enzyme-catalyzed functional lipid synthesis.

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Section: Discussionmentioning

confidence: 99%

Enzyme engineering for functional lipids synthesis: recent advance and perspective

Guan,

Hou,

Yang

et al. 2024

Bioresour. Bioprocess.

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Combined cross-linking of Rhizomucor miehei lipase and Candida antarctica lipase B for the effective enrichment of omega-3 fatty acids in fish oil

Ahrari,

Mohammadi

2024

International Journal of Biological Macromolecules

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Characterization of a New Thermostable and Organic Solution-Tolerant Lipase from Pseudomonas fluorescens and Its Application in the Enrichment of Polyunsaturated Fatty Acids

Jiao

Xie

et al. 2023

IJMS

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The search for and characterization of new lipases with excellent properties has always been urgent and is of great importance to meet industrial needs. In this study, a new lipase, lipB, from Pseudomonas fluorescens SBW25, belonging to the lipase subfamily I.3, was cloned and expressed in Bacillus subtilis WB800N. Enzymatic properties studies of recombinant LipB found that it exhibited the highest activity towards p-nitrophenyl caprylate at 40 °C and pH 8.0, retaining 73% of its original activity after incubation at 70 °C for 6 h. In addition, Ca2+, Mg2+, and Ba2+ strongly enhanced the activity of LipB, while Cu2+, Zn2+, Mn2+, and CTAB showed an inhibiting effect. The LipB also displayed noticeable tolerance to organic solvents, especially acetonitrile, isopropanol, acetone, and DMSO. Moreover, LipB was applied to the enrichment of polyunsaturated fatty acids from fish oil. After hydrolyzing for 24 h, it could increase the contents of polyunsaturated fatty acids from 43.16% to 72.18%, consisting of 5.75% eicosapentaenoic acid, 19.57% docosapentaenoic acid, and 46.86% docosahexaenoic acid, respectively. The properties of LipB render it great potential in industrial applications, especially in health food production.

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Omega-3 production by fish oil hydrolysis using a lipase from Burkholderia gladioli BRM58833 immobilized and stabilized by post-immobilization techniques

Cited by 3 publications

References 22 publications

Enzyme engineering for functional lipids synthesis: recent advance and perspective

Enzyme engineering for functional lipids synthesis: recent advance and perspective

Combined cross-linking of Rhizomucor miehei lipase and Candida antarctica lipase B for the effective enrichment of omega-3 fatty acids in fish oil

Characterization of a New Thermostable and Organic Solution-Tolerant Lipase from Pseudomonas fluorescens and Its Application in the Enrichment of Polyunsaturated Fatty Acids

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