Omega‐3 Polyunsaturated Fatty Acids Inhibit IL‐17A Secretion through Decreased ICAM‐1 Expression in T Cells Co‐Cultured with Adipose‐Derived Stem Cells Harvested from Adipose Tissues of Obese Subjects

Chehimi, Marwa; Ward, Robert E.; Pestel, Julien; Robert, M.; Pesenti, Sandra; Bendridi, Nadia; Michalski, Marie‐Caroline; Laville, Martine; Vidal, Hubert; Eljaafari, Assia

doi:10.1002/mnfr.201801148

Cited by 15 publications

(13 citation statements)

References 53 publications

(115 reference statements)

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“…Whereas markers of platelet aggregation and activation increased in activated ASC/MNC co-cultures, no difference was observed whether G-HSA or HSA was present. ICAM-1 expression, which has also been shown to increase in endothelial cells under the influence of RAGE activation[ 30 ] and in co-cultures of obese ASC with T cells[ 31 ], did not increase in the presence of G-HSA.…”

Section: Resultsmentioning

confidence: 99%

Involvement of glycated albumin in adipose-derived-stem cell-mediated interleukin 17 secreting T helper cell activation

Pestel¹,

Robert²,

Corbin³

et al. 2020

WJSC

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BACKGROUND Advanced glycation end products (AGE) are a marker of various diseases including diabetes, in which they participate to vascular damages such as retinopathy, nephropathy and coronaropathy. Besides those vascular complications, AGE are involved in altered metabolism in many tissues, including adipose tissue (AT) where they contribute to reduced glucose uptake and attenuation of insulin sensitivity. AGE are known to contribute to type 1 diabetes (T1D) through promotion of interleukin (IL)-17 secreting T helper (Th17) cells. AIM To investigate whether lean adipose-derived stem cells (ASC) could be able to induce IL-17A secretion, with the help of AGE. METHODS As we have recently demonstrated that ASC are involved in Th17 cell promotion when they are harvested from obese AT, we used the same co-culture model to measure the impact of glycated human serum albumin (G-HSA) on human lean ASC interacting with blood mononuclear cells. IL-17A and pro-inflammatory cytokine secretion were measured by ELISA. Receptor of AGE (RAGE) together with intercellular adhesion molecule 1 (ICAM-1), human leukocyte Antigen (HLA)-DR, cluster of differentiation (CD) 41, and CD62P surface expressions were measured by cytofluorometry. Anti-RAGE specific monoclonal antibody was added to co-cultures in order to evaluate the role of RAGE in IL-17A production. RESULTS Results showed that whereas 1% G-HSA only weakly potentiated the production of IL-17A by T cells interacting with ASC harvested from obese subjects, it markedly increased IL-17A, but also interferon gamma and tumor necrosis factor alpha production in the presence of ASC harvested from lean individuals. This was associated with increased expression of RAGE and HLA-DR molecule by co-cultured cells. Moreover, RAGE blockade experiments demonstrated RAGE specific involvement in lean ASC-mediated Th-17 cell activation. Finally, platelet aggregation and ICAM-1, which are known to be induced by AGE, were not involved in these processes. CONCLUSION Thus, our results demonstrated that G-HSA potentiated lean ASC-mediated IL-17A production in AT, suggesting a new mechanism by which AGE could contribute to T1D pathophysiology.

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Section: Resultsmentioning

confidence: 99%

Involvement of glycated albumin in adipose-derived-stem cell-mediated interleukin 17 secreting T helper cell activation

Pestel¹,

Robert²,

Corbin³

et al. 2020

WJSC

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“…There were no analyses of cell function, such as cytokine secretion, because these measurements were not required to address the primary aim of the study. Nevertheless, previous studies have shown that omega-3 PUFA-induced changes in cytokine mRNA levels, including IL-17A and IL-5 in human [ 55 ] and murine [ 56 ] T cells or PBMCs, are related directly to their concentration in cell culture supernatants. Therefore, although the present study did not investigate whether changes in cytokine mRNA levels were associated with differences in their respective concentrations, it is probable that differences in gene expression would have altered cytokine secretion.…”

Section: Discussionmentioning

confidence: 99%

Dietary Supplementation with Transgenic Camelina sativa Oil Containing 20:5n-3 and 22:6n-3 or Fish Oil Induces Differential Changes in the Transcriptome of CD3+ T Lymphocytes

et al. 2021

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Eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) are important for leukocyte function. This study investigated whether consuming transgenic Camelina sativa (tCSO) seed oil containing both 20:5n-3 and 22:6n-3 is as effective as fish oil (FO) for increasing the 20:5n-3 and 22:6n-3 content of leukocytes and altering mitogen-induced changes to the T cell transcriptome. Healthy adults (n = 31) consumed 450 mg/day of 20:5n-3 plus 22:6n-3 from either FO or tCSO for 8 weeks. Blood was collected before and after the intervention. 20:5n-3 and 22:6n-3 incorporation from tCSO into immune cell total lipids was comparable to FO. The relative expression of the transcriptomes of mitogen-stimulated versus unstimulated T lymphocytes in a subgroup of 16 women/test oil showed 4390 transcripts were differentially expressed at Baseline (59% up-regulated), 4769 (57% up-regulated) after FO and 3443 (38% up-regulated) after tCSO supplementation. The 20 most altered transcripts after supplementation differed between test oils. The most altered pathways were associated with cell proliferation and immune function. In conclusion, 20:5n-3 and 22:6n-3 incorporation into immune cells from tCSO was comparable to FO and can modify mitogen-induced changes in the T cell transcriptome, contingent on the lipid matrix of the oil.

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“…Several investigators have reported decreased expression of genes encoding proteins involved in pro-inflammatory pathways in human, animal and cellular models in response to EPA and/or DHA. For example, a recent study revealed beneficial effects of n-3 PUFA in IL-17A-related inflammatory pathologies through down-regulation of intercellular adhesion molecule-1 expression in monocytes and adipose-derived stem cells (36) . In contrast, adipocyte-derived PGE 2 , a derivative of ARA, mediates recruitment of adipose tissue macrophages contributing to inflammation (37) .…”

Section: Discussionmentioning

confidence: 99%

Maternal high-fat diet in mice alters immune regulation and lung function in the offspring

et al. 2020

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Polyunsaturated fatty acids (PUFA) modulate immune function and have been associated with risk of childhood atopy and asthma. We investigated the effect of maternal fat intake in mice on PUFA status, elongase and desaturase gene expression, inflammatory markers and lung function in the offspring. C57BL/6J mice (n=32) were fed either standard chow (C, 21% kcal fat) or a high fat diet (HFD, 45% kcal fat) for 4 weeks prior to conception and during gestation and lactation. At 21 days of age, offspring were weaned onto either the HFD or C, generating four experimental groups: C/C, C/HF, HF/C and HF/HF. Plasma and liver fatty acid composition were measured by gas chromatography and gene expression by qPCR. Lung resistance to methacholine was assessed. Arachidonic acid concentrations in offspring plasma and liver phospholipids were increased by HFD; this effect was greater in the post-natal HFD group. Docosahexaenoic acid concentration in offspring liver phospholipids was increased in response to HFD and was higher in the post-natal HFD group. Post-natal HFD increased hepatic FADS2 and ELOVL5 expression in male offspring, whereas maternal HFD elevated expression of FADS1 and FADS2 in female offspring comparing to males. Post-natal HFD increased expression of IL-6 and CCL2 in perivascular adipose tissue. The HFD lowered lung resistance to methacholine. Excessive maternal fat intake during development modifies hepatic PUFA status in offspring through regulation of gene expression of enzymes that are involved in PUFA biosynthesis and modifies the development of the offspring lungs leading to respiratory dysfunction.

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Omega‐3 Polyunsaturated Fatty Acids Inhibit IL‐17A Secretion through Decreased ICAM‐1 Expression in T Cells Co‐Cultured with Adipose‐Derived Stem Cells Harvested from Adipose Tissues of Obese Subjects

Cited by 15 publications

References 53 publications

Involvement of glycated albumin in adipose-derived-stem cell-mediated interleukin 17 secreting T helper cell activation

Involvement of glycated albumin in adipose-derived-stem cell-mediated interleukin 17 secreting T helper cell activation

Dietary Supplementation with Transgenic Camelina sativa Oil Containing 20:5n-3 and 22:6n-3 or Fish Oil Induces Differential Changes in the Transcriptome of CD3+ T Lymphocytes

Maternal high-fat diet in mice alters immune regulation and lung function in the offspring

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