Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa

Kraatz, Mareike; Wallace, R. J.; Svensson, Lars

doi:10.1099/ijs.0.022954-0

Cited by 121 publications

(107 citation statements)

References 47 publications

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“…Another bacterium found to be associated with PC in this study and which is also associated with human caries is Olsenella spp; a small, rod‐shaped microaerotolerant (moderately obligate) anaerobic bacterium which can ferment carbohydrates, predominantly to lactic acid but also to formic and acetic acids. Olsenella spp, such as O. profusa are associated with human caries affecting dentine , as well as root (i.e.…”

Section: Discussionmentioning

confidence: 74%

A microbiome analysis of equine peripheral dental caries using next generation sequencing

Borkent

Reardon

McLachlan

et al. 2019

Equine Veterinary Journal

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Summary Background Although, peripheral caries (PC) affects almost half of UK horses, no comprehensive microbiological study has been performed on this disorder. As a high proportion of oral bacteria cannot be conventionally cultured, molecular microbiological techniques such as Next Generation Sequencing are required to examine the complex oral bacteria community. Objectives To identify the microbiota involved in equine PC, including comparing microbiota at the more commonly and severely affected three caudal cheek teeth with the less commonly affected three rostral cheek teeth. Study design and Methods Equine dental plaque samples were collected from the palatal aspects of cheek teeth of 63 horses. DNA was isolated and amplified using PCR, targeting the V4 region of the 16S rRNA gene and Next Generation Sequencing of these gene amplicons was performed. The acquired data were processed and analysed using Mothur and R. Results Streptococcus species was the genus most commonly associated with equine PC, whereas Gemella species was the genus most associated with the control group. In a further analysis where the rostral and caudal cheek teeth were compared with each other and with the control group. Veillonella species was the most commonly associated genus with PC of the rostral cheek teeth, Streptococcus species was the most associated genus with the caudal cheek teeth, and Corynebacterium with the control group. Main limitations Some bacteria can have multiple heterogeneous copies of the 16S rRNA gene, which can affect the estimation of their relative abundance. Conclusions Similar to caries studies in other species, acidogenic and aciduric microorganisms including Streptococcus species were found to be associated with equine peripheral caries.

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Section: Discussionmentioning

confidence: 74%

A microbiome analysis of equine peripheral dental caries using next generation sequencing

Borkent

Reardon

McLachlan

et al. 2019

Equine Veterinary Journal

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“…Two other bacterial phyla that were significantly more abundant in white water than in clear water sediments were the Actinobacteria (average 8.3%) and the Chloroflexi (average 5.3%). Species of the actinobacterial genus Olsenella are lactic acid bacteria isolated from animal guts or oral cavities (Kraatz et al ., ). However, Olsenella has also been detected in groundwater (Bowman et al ., ) and anaerobic digestors (Riviere et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Structure and function of methanogenic microbial communities in sediments of Amazonian lakes with different water types

Yang

Angel

Klose

et al. 2016

Environmental Microbiology

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Tropical lake sediments are a significant source for the greenhouse gas methane. We studied function (pathway, rate) and structure (abundance, taxonomic composition) of the microbial communities (Bacteria, Archaea) leading to methane formation together with the main physicochemical characteristics in the sediments of four clear water, six white water and three black water lakes of the Amazon River system. Concentrations of sulfate and ferric iron, pH and δ C of organic carbon were usually higher, while concentrations of carbon, nitrogen and rates of CH production were generally lower in white water versus clear water or black water sediments. Copy numbers of bacterial and especially archaeal ribosomal RNA genes also tended to be relatively lower in white water sediments. Hydrogenotrophic methanogenesis contributed 58 ± 16% to total CH production in all systems. Network analysis identified six communities, of which four were comprised mostly of bacteria found in all sediment types, while two were mostly in clear water sediment. Terminal restriction fragment length polymorphism (T-RFLP) and pyrosequencing showed that the compositions of the communities differed between the different sediment systems, statistically related to the particular physicochemical conditions and to CH production rates. Among the archaea, clear water, white water, and black water sediments contained relatively more Methanomicrobiales, Methanosarcinaceae and Methanocellales, respectively, while Methanosaetaceae were common in all systems. Proteobacteria, Deltaproteobacteria (Myxococcales, Syntrophobacterales, sulfate reducers) in particular, Acidobacteria and Firmicutes were the most abundant bacterial phyla in all sediment systems. Among the other important bacterial phyla, clear water sediments contained relatively more Alphaproteobacteria and Planctomycetes, whereas white water sediments contained relatively more Betaproteobacteria, Firmicutes, Actinobacteria, and Chloroflexi than the respective other sediment systems. The data showed communities of bacteria common to all sediment types, but also revealed microbial groups that were significantly different between the sediment types, which also differed in physicochemical conditions. Our study showed that function of the microbial communities may be understood on the basis of their structures, which in turn are determined by environmental heterogeneity.

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“…Atopobium was proposed as a new genus derived from Lactobacillus and Streptococcus with the type strain Atopobium minutum in 1992 based on comparative sequence analyses [26]. A close relationship between Atopobium and Olsenella was shown in the bigeneric Olsenella - Atopobium branch of the family Coriobacteriaceae, class Actinobacteria [27], which might have caused misidentification in our first RDP pyrosequencing pipeline attempt. We confirmed that the RDP Classifier misclassified the V1–V2 region of the 16S rRNA gene of Atopobium sp.…”

Section: Discussionmentioning

confidence: 98%

Identification of the Microbiota in Carious Dentin Lesions Using 16S rRNA Gene Sequencing

et al. 2014

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While mutans streptococci have long been assumed to be the specific pathogen responsible for human dental caries, the concept of a complex dental caries-associated microbiota has received significant attention in recent years. Molecular analyses revealed the complexity of the microbiota with the predominance of Lactobacillus and Prevotella in carious dentine lesions. However, characterization of the dentin caries-associated microbiota has not been extensively explored in different ethnicities and races. In the present study, the bacterial communities in the carious dentin of Japanese subjects were analyzed comprehensively with molecular approaches using the16S rRNA gene. Carious dentin lesion samples were collected from 32 subjects aged 4–76 years, and the 16S rRNA genes, amplified from the extracted DNA with universal primers, were sequenced with a pyrosequencer. The bacterial composition was classified into clusters I, II, and III according to the relative abundance (high, middle, low) of Lactobacillus. The bacterial composition in cluster II was composed of relatively high proportions of Olsenella and Propionibacterium or subdominated by heterogeneous genera. The bacterial communities in cluster III were characterized by the predominance of Atopobium, Prevotella, or Propionibacterium with Streptococcus or Actinomyces. Some samples in clusters II and III, mainly related to Atopobium and Propionibacterium, were novel combinations of microbiota in carious dentin lesions and may be characteristic of the Japanese population. Clone library analysis revealed that Atopobium sp. HOT-416 and P. acidifaciens were specific species associated with dentinal caries among these genera in a Japanese population. We summarized the bacterial composition of dentinal carious lesions in a Japanese population using next-generation sequencing and found typical Japanese types with Atopobium or Propionibacterium predominating.

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Olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of Olsenella, Olsenella uli and Olsenella profusa

Cited by 121 publications

References 47 publications

A microbiome analysis of equine peripheral dental caries using next generation sequencing

A microbiome analysis of equine peripheral dental caries using next generation sequencing

Structure and function of methanogenic microbial communities in sediments of Amazonian lakes with different water types

Identification of the Microbiota in Carious Dentin Lesions Using 16S rRNA Gene Sequencing

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