Olive Tree Genomic

Muleo, Rosario; Morgante, Michèle; Velasco, Riccardo; Cavallini, Andrea; Perrotta, Gaetano; Baldoni, Luciana

doi:10.5772/51720

Cited by 15 publications

(9 citation statements)

References 26 publications

(16 reference statements)

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“…These results appear even more interesting in the light of the results of the neutrality tests, showing significant and positive variations for both OeACP1 and OeACP2 loci as a possible consequence of a recent bottleneck and balancing selection followed by a steady varieties cultivation (Maruyama and Fuerst, 1985). Despite this, the olive genome sequencing data of the Leccino and Farga cultivars (Muleo et al, 2012; Cruz et al, 2016) and the assembled genes of a wild olive (Unver et al, 2017), as well as the lack of saturated genetic maps, have not allowed to physically map these genes.…”

Section: Discussionmentioning

confidence: 99%

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High Levels of Variation Within Gene Sequences of Olea europaea L.

et al. 2019

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Gene sequence variation in cultivated olive (Olea europaea L. subsp. europaea var. europaea), the most important oil tree crop of the Mediterranean basin, has been poorly evaluated up to now. A deep sequence analysis of fragments of four genes, OeACP1, OeACP2, OeLUS and OeSUT1, in 90 cultivars, revealed a wide range of polymorphisms along all recognized allele forms and unexpected allele frequencies and genotype combinations. High linkage values among most polymorphisms were recorded within each gene fragment. The great sequence variability corresponded to a low number of alleles and, surprisingly, to a small fraction of genotype combinations. The distribution, frequency, and combination of the different alleles at each locus is possibly due to natural and human pressures, such as selection, ancestrality, or fitness. Phylogenetic analyses of allele sequences showed distant and complex patterns of relationships among cultivated olives, intermixed with other related forms, highlighting an evolutionary connection between olive cultivars and the O. europaea subspecies cuspidata and cerasiformis. This study demonstrates how a detailed and complete sequence analysis of a few gene portions and a thorough genotyping on a representative set of cultivars can clarify important issues related to sequence polymorphisms, reconstructing the phylogeny of alleles, as well as the genotype combinations. The identification of regions representing blocks of recombination could reveal polymorphisms that represent putatively functional markers. Indeed, specific mutations found on the analyzed OeACP1 and OeACP2 fragments seem to be correlated to the fruit weight.

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Section: Discussionmentioning

confidence: 99%

“…The obtained sequences were used as queries in a BLAST search on the genome of the cultivars Farga ( 3 Cruz et al, 2016) and Leccino ( 4 Muleo et al, 2012), and O. europaea subsp. europaea var.…”

Section: Methodsmentioning

confidence: 99%

High Levels of Variation Within Gene Sequences of Olea europaea L.

et al. 2019

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“…There are currently more than 2000 olive cultivars and this genetic diversity is the main factor contributing to the olive oil singularity of each country and region [ 4 , 5 ]. Besides the organoleptic characteristics of fruits and oils produced, genetic diversity also represents a source of genetic information that could be further explored in breeding programs focused on specific agronomical traits [ 6 ]. Development of new cultivars must be focused on solving issues such as modification of growth habit, development of self-fertile plants to increase yield, development of totally self-sterile plants for conventional breeding, increasing fruit oil content and quality, increasing abiotic stress tolerance, increasing disease and pest tolerance, and production of plants with a higher adventitious rooting ability [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis from Mature Embryos of Olea europaea L. cv. ‘Galega Vulgar’ and Long-Term Management of Calli Morphogenic Capacity

Pires

Cardoso

Ribeiro

et al. 2020

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Several olive cultivars, characterized by high-quality olive oil show agronomical issues such as excessive vigor, high susceptibility to biotic and abiotic stresses, and low propagation ability. They are strong candidates for breeding based on new technologies to improve their performance in a short period of time. For this reason, the first step is developing efficient somatic embryogenesis (SE) protocols. Somatic embryogenesis in olive is highly genotype-dependent for both adult tissues and mature embryos as initial explants, requiring the development of specific protocols for each genotype. Trials using cotyledons and radicles as initial explants, isolated from ripe seeds from the Portuguese olive cv. ‘Galega vulgar’, gave more than 95% calli development. Radicles proved to be the most responsive tissue for SE induction, with an average of 2 embryos per callus after callus transfer to expression medium, and 14 embryos per callus after subculture on the olive cyclic embryogenesis medium (ECO). Embryogenic competence could be recovered after several subcultures on ECO medium that maintained cyclic embryogenesis for an indeterminate period of time. Embryo conversion and plant acclimatization were also attained with high success rates. Media management for cyclic embryogenesis maintenance is of general importance for SE protocols in any olive genotype. Somatic embryogenesis was thus attained for the first time in embryo-derived explants of cv. ‘Galega vulgar’.

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“…Olive tree ( Olea europaea L.) is one of the most important oil-producing plant species all over the world. While waiting for the genome sequence ( Muleo et al, 2012 ), transcriptomic approaches have been exploited. For example, subtractive libraries from olive fruits sampled at three different stages shed light on metabolic pathways and transcriptional aspects related to carbohydrates, fatty acids, secondary metabolites, transcription factors, and hormones as well as response to biotic and abiotic stresses throughout olive drupe development ( Galla et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

ReprOlive: a database with linked data for the olive tree (Olea europaea L.) reproductive transcriptome

et al. 2015

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Plant reproductive transcriptomes have been analyzed in different species due to the agronomical and biotechnological importance of plant reproduction. Here we presented an olive tree reproductive transcriptome database with samples from pollen and pistil at different developmental stages, and leaf and root as control vegetative tissues http://reprolive.eez.csic.es). It was developed from 2,077,309 raw reads to 1,549 Sanger sequences. Using a pre-defined workflow based on open-source tools, sequences were pre-processed, assembled, mapped, and annotated with expression data, descriptions, GO terms, InterPro signatures, EC numbers, KEGG pathways, ORFs, and SSRs. Tentative transcripts (TTs) were also annotated with the corresponding orthologs in Arabidopsis thaliana from TAIR and RefSeq databases to enable Linked Data integration. It results in a reproductive transcriptome comprising 72,846 contigs with average length of 686 bp, of which 63,965 (87.8%) included at least one functional annotation, and 55,356 (75.9%) had an ortholog. A minimum of 23,568 different TTs was identified and 5,835 of them contain a complete ORF. The representative reproductive transcriptome can be reduced to 28,972 TTs for further gene expression studies. Partial transcriptomes from pollen, pistil, and vegetative tissues as control were also constructed. ReprOlive provides free access and download capability to these results. Retrieval mechanisms for sequences and transcript annotations are provided. Graphical localization of annotated enzymes into KEGG pathways is also possible. Finally, ReprOlive has included a semantic conceptualisation by means of a Resource Description Framework (RDF) allowing a Linked Data search for extracting the most updated information related to enzymes, interactions, allergens, structures, and reactive oxygen species.

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Olive Tree Genomic

Cited by 15 publications

References 26 publications

High Levels of Variation Within Gene Sequences of Olea europaea L.

High Levels of Variation Within Gene Sequences of Olea europaea L.

Somatic Embryogenesis from Mature Embryos of Olea europaea L. cv. ‘Galega Vulgar’ and Long-Term Management of Calli Morphogenic Capacity

ReprOlive: a database with linked data for the olive tree (Olea europaea L.) reproductive transcriptome

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