Olive pollen allergy: searching for immunodominant T‐cell epitopes on the Ole e 1 molecule

Cárdaba, Blanca; Am, del Pozo; Jurado, Aurora; Gallardo, Soledad; Cortegano, Isabel; Arrieta, Ignacio; A, Del Amo; Tramón, Paloma; Florido, Fernando; Sastre, Joaquı́n; Palomino, Pilar; Lahoz, Carlos

doi:10.1046/j.1365-2222.1998.00190.x

Cited by 40 publications

(38 citation statements)

References 40 publications

(48 reference statements)

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“…However, our results (Fig. 2) and the data of others (35)(36)(37) indicate that the amino acid sequence per se cannot determine the allergenicity of a protein because allergic and nonallergic people often recognize the same epitopes or regions in the molecule. One possibility to explain the Th2-deviated immune response by atopic individuals to a lipocalin allergen could be the way the allergen peptide is recognized by Th cells.…”

Section: Discussioncontrasting

confidence: 45%

T Cell Epitope-Containing Peptides of the Major Dog Allergen Can f 1 as Candidates for Allergen Immunotherapy

Immonen

Farci

Taivainen

et al. 2005

The Journal of Immunology

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One prerequisite for developing peptide-based allergen immunotherapy is knowing the T cell epitopes of an allergen. In this study, human T cell reactivity against the major dog allergen Can f 1 was investigated to determine peptides suitable for immunotherapy. Seven T cell epitope regions (A–G) were found in Can f 1 with specific T cell lines and clones. The localization of the epitope regions shows similarities with those of the epitopes found in Bos d 2 and Rat n 1. On average, individuals recognized three epitopes in Can f 1. Our results suggest that seven 16-mer peptides (p15–30, p33–48, p49–64, p73–88, p107–122, p123–138, and p141–156), each from one of the epitope regions, show widespread T cell reactivity in the population studied, and they bind efficiently to seven HLA-DRB1 molecules (DRB1*0101, DRB1*0301, DRB1*0401, DRB1*0701, DRB1*1101, DRB1*1301, and DRB1*1501) predominant in Caucasian populations. Therefore, these peptides are potential candidates for immunotherapy of dog allergy.

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Section: Discussioncontrasting

confidence: 45%

T Cell Epitope-Containing Peptides of the Major Dog Allergen Can f 1 as Candidates for Allergen Immunotherapy

Immonen

Farci

Taivainen

et al. 2005

The Journal of Immunology

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“…Studies using lymphoproliferation assays to map T cell antigenic determinants of Ags associated with DTH have demonstrated differences in the T cell repertoire in humans with different disease states (17)(18)(19)(20)(21). However, most studies of T cell responses to allergens have not shown significant differences in T cell repertoires between atopic and nonatopic subjects (22)(23)(24).…”

mentioning

confidence: 99%

Distinct Human T Cell Repertoires Mediate Immediate and Delayed-Type Hypersensitivity to theTrichophytonAntigen, Tri r 2

Woodfolk¹,

Sung²,

Benjamin

et al. 2000

The Journal of Immunology

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The 29-kDa subtilase homologue, Tri r 2, derived from the dermatophyte fungus Trichophyton rubrum, exhibits unique immunologic characteristics in its ability to elicit immediate (IH) and delayed-type (DTH) hypersensitivity skin tests in different individuals. Thus, Tri r 2 provides a model for comparing the T cell repertoire in subjects with distinct immune responses to a single Ag. Recombinant Tri r 2 produced as a GST fusion protein in Escherichia coli stimulated strong in vitro lymphoproliferative responses in 10 IH and 10 DTH responders. Patterns of T cell epitope recognition were compared between skin test groups using 28 overlapping peptides (each in 12 replicate wells) derived from Tri r 2 to stimulate T lymphocyte proliferation in vitro. Peptide 5 (P5; aa 41–60) induced the strongest response in DTH subjects and showed the largest difference between DTH and IH responders in proliferation (mean standardized index, 2.22 and 0.82, respectively; p = 0.0047) and number of positive wells (81 vs 12). Responses to P5 were associated with diverse HLA haplotypes. These results showed that P5 contains an immunodominant epitope specifically associated with DTH and that this peptide is recognized in a permissive manner. Cross-validated linear discriminant analysis using T cell proliferative responses to two regions of Tri r 2 (aa 51–90 and 231–270) gave a 95% predictive accuracy for classification of subjects into IH or DTH groups. We conclude that different immune responses to Trichophyton are mediated by distinct T cell repertoires between individuals with IH and DTH reactions to Tri r 2.

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“…Peptides 4 and 5 contained previously described T-cell epitopes of Ole e 1 (Fig 1). 14 The molecular weights of the peptides ranged from 3511.9 Da (peptide 2) to 4069.6 Da (peptide 1). Peptides 1, 2, and 3 were acidic, with calculated isoelectric points of less than 5.0, whereas peptides 4 and 5 were basic, with isoelectric points of greater than 9.0 (Table E4).…”

Section: Characterization Of Ole E 1-derived Peptidesmentioning

confidence: 99%

“…12,13 Therefore several attempts have been made to characterize IgE epitopes and T-cell epitopes of Ole e 1 for the development of a molecule-based therapeutic strategy. 7,14,15 With the aim of reducing IgE-mediated side effects during SIT, 2 approaches have thus far been pursued. One approach was based on the use of T cell-reactive Ole e 1 peptides 16 without IgE for inducing T-cell tolerance.…”

mentioning

confidence: 99%

Carrier-bound, nonallergenic Ole e 1 peptides for vaccination against olive pollen allergy

Twaroch

Focke

Civaj

et al. 2011

Journal of Allergy and Clinical Immunology

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Olive pollen allergy: searching for immunodominant T‐cell epitopes on the Ole e 1 molecule

Abstract: Our data suggest that the regions between 91 and 102 and 109-130 aminoacids on the Ole e 1 molecule are immunodominant T-cell epitopes. These epitopes are not recognized by IgE antibodies.

Cited by 40 publications

References 40 publications

T Cell Epitope-Containing Peptides of the Major Dog Allergen Can f 1 as Candidates for Allergen Immunotherapy

T Cell Epitope-Containing Peptides of the Major Dog Allergen Can f 1 as Candidates for Allergen Immunotherapy

Distinct Human T Cell Repertoires Mediate Immediate and Delayed-Type Hypersensitivity to theTrichophytonAntigen, Tri r 2

Carrier-bound, nonallergenic Ole e 1 peptides for vaccination against olive pollen allergy

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