Olive oil oleuropein has anti-breast cancer properties with higher efficiency on ER-negative cells

Elamin, Maha H.; Daghestani, Maha H.; Omer, Sawsan A.; Elobeid, Mai; Virk, Promy; Al-Olayan, Ebtesam M.; Hassan, Zeinab K.; Mohammed, Osama B.; Aboussekhra, Abdelilah

doi:10.1016/j.fct.2012.12.009

Cited by 114 publications

(85 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…7A). Likewise, Elamin et al have described that oleuropein induced apoptosis via the mitochondrial pathway in MCF-7 cells (58). It has been reported that genipin induces apoptotic cell death in PC3 cells (59) and HeLa cells (35), via JNK-dependent activation of the mitochondrial pathway.…”

Section: Discussionmentioning

confidence: 96%

Oleuropein Induced Apoptosis in HeLa Cells via a Mitochondrial Apoptotic Cascade Associated With Activation of the c-Jun NH2-Terminal Kinase

Yao

Yang

et al. 2014

J Pharmacol Sci

View full text Add to dashboard Cite

Abstract. Oleuropein could inhibit growth and/or induce apoptosis in several cancer cell lines. In this study, we investigate how oleuropein strongly induces apoptotic cell death in HeLa human cervical carcinoma cells. Oleuropein induced HeLa cells apoptosis as demonstrated by induction of a sub-G 1 peak in flow cytometry and apoptosis-related morphological changes observed by fluorescence microscopy after being stained by Hoechst 33324. The results also showed that 150 -200 mM oleuropein-treated HeLa cells were arrested at the G 2 /M phase. Western blot analysis revealed that the phosphorylated ATF-2, c-Jun NH 2 -terminal kinase (JNK) protein, p53, p21, Bax, and cytochrome c protein in the cytoplasm significantly increased in a dose-dependent manner after treatment of oleuropein for 24 h. Additionally, increasing levels of Bax in response to JNK/SPAK signaling, which formed mitochondrial membrane channels, accounted for releasing of cytochrome c and activation of caspase-9 and -3. SP600125 (20 mM), a JNK 1/2 inhibitor, markedly suppressed the formation of apoptotic bodies and JNK activation induced by oleuropein at 200 mM. Thus, oleuropein-induced apoptosis was activated by the JNK/SPAK signal pathway. The result shows that oleuropein holds promise as a potential chemotherapeutic agent for the treatment of HeLa cells.

show abstract

Section: Discussionmentioning

confidence: 96%

Oleuropein Induced Apoptosis in HeLa Cells via a Mitochondrial Apoptotic Cascade Associated With Activation of the c-Jun NH2-Terminal Kinase

Yao

Yang

et al. 2014

J Pharmacol Sci

View full text Add to dashboard Cite

show abstract

“…Oleuropein can also target steps in cancer progression (Abe et al, 2011). Nowadays, oleuropein has garnered interest due to its ability to inhibit proliferation, metastasis and promote apoptosis in several tumor cell lines (Hassan et al, 2012;Zare et al, 2012;Elamin et al, 2013;Kostomoiri et al, 2013;Oi-Kano et al, 2013;Parzonko et al, 2013). In our previous study, oleuropein showed cytotoxic effect on the luminal MCF-7 cells via the induction of the mitochondrial apoptotic pathway (Elamin et al, 2013).…”

Section: Discussionmentioning

confidence: 98%

“…Cells were treated with 100µM or 200µM concentration of Oleuropein. The incubation period and oleuropein concentrations were determined according to our previous study (Elamin et al, 2013). The untreated cells were used as control.…”

Section: Methodsmentioning

confidence: 99%

“…oleuropein. In breast cancer cells, oleuropein decreases cell viability (Menendez et al, 2007), acts as an anti-metastatic agent (Hassan et al, 2012), and induces cell apoptosis (Campolo et al, 2013;Elamin et al, 2013;Oi-Kano et al, 2013;Pasban-Aliabadi et al, 2013). Oleuropein inhibits cell proliferation via delaying the cell cycle at S phase and up-regulating cyclin-dependent inhibitor p21 (Elamin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oleuropein Induces Apoptosis Via the p53 Pathway in Breast Cancer Cells

Hassan¹,

Elamin²,

Omer³

et al. 2013

Asian Pacific Journal of Cancer Prevention

Self Cite

View full text Add to dashboard Cite

“…The concentration 50-200 µg/mL was chosen in order to show the range in which the extracts had activity on the cells. This was based on previously published data on the anti-proliferative activity of olive leaf extracts for breast cancer [30]. After 96 h, 10 µL of CCK-8 solution was added before incubating at 37 °C for 120 min.…”

Section: Assessment Of Cell Growth Inhibition Of Olive Leaf Extractsmentioning

confidence: 99%

Phytochemical Properties and Anti-Proliferative Activity of Olea europaea L. Leaf Extracts against Pancreatic Cancer Cells

Goldsmith¹,

Vuong²,

Sadeqzadeh

et al. 2015

Molecules

View full text Add to dashboard Cite

Olea europaea L. leaves are an agricultural waste product with a high concentration of phenolic compounds; especially oleuropein. Oleuropein has been shown to exhibit anti-proliferative activity against a number of cancer types. However, they have not been tested against pancreatic cancer, the fifth leading cause of cancer related death in Western countries. Therefore, water, 50% ethanol and 50% methanol extracts of Corregiola and Frantoio variety Olea europaea L. leaves were investigated for their total phenolic compounds, total flavonoids and oleuropein content, antioxidant capacity and anti-proliferative activity against MiaPaCa-2 pancreatic cancer cells. The extracts only had slight differences in their phytochemical properties, and at 100 and 200 μg/mL, all decreased the viability of the pancreatic cancer cells relative to controls. At 50 μg/mL, the water extract from the Corregiola leaves exhibited the highest anti-proliferative activity with the effect possibly due to early eluting HPLC peaks. For this reason, olive leaf extracts warrant further investigation into their potential anti-pancreatic cancer benefits.

show abstract

Olive oil oleuropein has anti-breast cancer properties with higher efficiency on ER-negative cells

Cited by 114 publications

References 34 publications

Oleuropein Induced Apoptosis in HeLa Cells via a Mitochondrial Apoptotic Cascade Associated With Activation of the c-Jun NH2-Terminal Kinase

Oleuropein Induced Apoptosis in HeLa Cells via a Mitochondrial Apoptotic Cascade Associated With Activation of the c-Jun NH2-Terminal Kinase

Oleuropein Induces Apoptosis Via the p53 Pathway in Breast Cancer Cells

Phytochemical Properties and Anti-Proliferative Activity of Olea europaea L. Leaf Extracts against Pancreatic Cancer Cells

Contact Info

Product

Resources

About