Olive mill wastewater and hydroxytyrosol inhibits atherogenesis in apolipoprotein E-deficient mice

Hara, Tomoya; Fukuda, Daiju; Ganbaatar, Byambasuren; Phương, Phạm Trần; Aini, Kunduziayi; Rahadian, Arief; Suto, Kumiko; Yagi, Shusuke; Kusunose, Kenya; Yamada, Hirotsugu; Soeki, Takeshi; Sata, Masataka

doi:10.1007/s00380-023-02290-5

Cited by 2 publications

(6 citation statements)

References 59 publications

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“…In such case, HT-mediated downregulation of this enzyme would be in agreement with a lower inflammatory state, consistent with the inhibition in the canonical pathway "production of nitric oxide and reactive oxygen species in macrophages" observed after the intake of HT in stroke patients (Figure 4). Moreover, this result would also agree with the attenuated inflammatory activation of macrophages and the reduced vascular inflammation recently described by Hara et al [29] in HT-treated mice. These data contrast with those observed in control patients.…”

Section: Discussionsupporting

confidence: 92%

“…Thus, the initial decrease in NO levels is followed by a progressive upregulation, in such a way that the increase from day 1 to 2 is beneficial to patients, but a later steep increase is detrimental. HT is a well-known antioxidant and a modulator of NO production [9,34] with an atheroprotective role achieved, among others, by a decreased expression of the inducible isoform of NO synthase (iNOS) and by the downregulation of oxidative stress [28,29]. In this study, we showed that the administration of HT modulated NO production (p = 0.001).…”

Section: Discussionmentioning

confidence: 55%

“…Although systolic blood pressure is considered to be more important than diastolic blood pressure in the determination of cardiovascular risk, Flit et al [27] found that systolic and diastolic hypertension independently predicted adverse outcomes, despite a greater effect of systolic hypertension. In two recent papers, Ikobomidies et al [28] and Hara et al [29] described that an HT-enriched olive oil and a 6% HT-enriched diet reduced the oxidative and inflammatory burden in chronic coronary artery syndrome patients and in apolipoprotein E-deficient mice, but had no effect on BP. In our study, HT did not modulate SBP, but seemed to induce a more prolonged decrease in DBP, resembling the pattern of response of HbA 1 c and reinforcing the positive effect of HT.…”

Section: Discussionmentioning

confidence: 99%

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Hydroxytyrosol, a Promising Supplement in the Management of Human Stroke: An Exploratory Study

Naranjo,

Álvarez-Soria,

Aranda-Villalobos

et al. 2024

IJMS

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Hydroxytyrosol (HT) is a bioactive olive oil phenol with beneficial effects in a number of pathological situations. We have previously demonstrated that an HT-enriched diet could serve as a beneficial therapeutic approach to attenuate ischemic-stroke-associated damage in mice. Our exploratory pilot study examined this effect in humans. Particularly, a nutritional supplement containing 15 mg of HT/day was administered to patients 24 h after the onset of stroke, for 45 days. Biochemical and oxidative-stress-related parameters, blood pressure levels, serum proteome, and neurological and functional outcomes were evaluated at 45 and 90 days and compared to a control group. The main findings were that the daily administration of HT after stroke could: (i) favor the decrease in the percentage of glycated hemoglobin and diastolic blood pressure, (ii) control the increase in nitric oxide and exert a plausible protective effect in oxidative stress, (iii) modulate the evolution of the serum proteome and, particularly, the expression of apolipoproteins, and (iv) be beneficial for certain neurological and functional outcomes. Although a larger trial is necessary, this study suggests that HT could be a beneficial nutritional complement in the management of human stroke.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 99%

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Hydroxytyrosol, a Promising Supplement in the Management of Human Stroke: An Exploratory Study

Naranjo,

Álvarez-Soria,

Aranda-Villalobos

et al. 2024

IJMS

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“…For example, HT has been shown to reduce blood pressure and improve endothelial function, which could help to prevent atherosclerosis and other cardiovascular diseases ( 45–48 ). Hara et al ( 41 ) investigated the effect of OMWW and HT on atherogenesis, OMWW (0.30% w/w) or HT (0.02% w/w) were added to a western-type diet for 20 weeks and fed to male apolipoprotein E-deficient mice. Without affecting body weight, plasma cholesterol levels, or blood pressure, OMWW and HT slowed the progression of atherosclerosis in the aortic arch, in a comparable manner.…”

Section: Review Methodologymentioning

confidence: 99%

“…The aorta’s generation of oxidative stress, as well as the expression of inflammatory molecules like IL-1 and MCP-1 and NADPH oxidase subunits like NOX2 and p22phox, were all reduced by OMWW and HT. ( 41 )…”

Section: Review Methodologymentioning

confidence: 99%

From antiquity to contemporary times: how olive oil by-products and waste water can contribute to health

Albini,

Corradino

et al. 2023

Front. Nutr.

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Since antiquity, numerous advantages of olive oil and its by-products have been recognized in various domains, including cooking, skincare, and healthcare. Extra virgin olive oil is a crucial component of the Mediterranean diet; several of its compounds exert antioxidant, anti-proliferative, anti-angiogenic and pro-apoptotic effects against a variety of cancers, and also affect cellular metabolism, targeting cancer cells through their metabolic derangements. Numerous olive tree parts, including leaves, can contribute metabolites useful to human health. Olive mill waste water (OMWW), a dark and pungent liquid residue produced in vast amounts during olive oil extraction, contains high organic matter concentrations that may seriously contaminate the soil and surrounding waters if not managed properly. However, OMWW is a rich source of phytochemicals with various health benefits. In ancient Rome, the farmers would employ what was known as amurca, a mulch-like by-product of olive oil production, for many purposes and applications. Several studies have investigated anti-angiogenic and chemopreventive activities of OMWW extracts. The most prevalent polyphenol in OMWW extracts is hydroxytyrosol (HT). Verbascoside and oleuperin are also abundant. We assessed the impact of one such extract, A009, on endothelial cells (HUVEC) and cancer cells. A009 was anti-angiogenic in several in vitro assays (growth, migration, adhesion) and inhibited angiogenesis in vivo, outperforming HT alone. A009 inhibited cells from several tumors in vitro and in vivo and showed potential cardioprotective effects mitigating cardiotoxicity induced by chemotherapy drugs, commonly used in cancer treatment, and reducing up-regulation of pro-inflammatory markers in cardiomyocytes. Extracts from OMWW and other olive by-products have been evaluated for biological activities by various international research teams. The results obtained make them promising candidates for further development as nutraceutical and cosmeceutical agents or dietary supplement, especially in cancer prevention or even in co-treatments with anti-cancer drugs. Furthermore, their potential to offer cardioprotective benefits opens up avenues for application in the field of cardio-oncology.

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Olive mill wastewater and hydroxytyrosol inhibits atherogenesis in apolipoprotein E-deficient mice

Cited by 2 publications

References 59 publications

Hydroxytyrosol, a Promising Supplement in the Management of Human Stroke: An Exploratory Study

Hydroxytyrosol, a Promising Supplement in the Management of Human Stroke: An Exploratory Study

From antiquity to contemporary times: how olive oil by-products and waste water can contribute to health

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