Olive leaf extract as a crosslinking agent for the preparation of electrospun zein fibers

Abstract

Incorporating active agents, reinforcing structure by crosslinking, thus changing release properties, can be listed as possible modifications in preparation methods of biopolymer fibers. This study introduces oleuropein, major component of olive leaf extract (OLE), as a natural functional crosslinker for electrospun zein fibers, owing to its antioxidant and antimicrobial properties. Incorporation of OLE causes morphological and structural changes indicated by a decrease in fiber diameter up to 27%, an increase in intensity of NH bending region due to interaction with -OH groups and observation of characteristic oleuropein bands. Extract addition also enhances thermal stability. Zein fibers without OLE is fully degraded at 600 C, whereas 10% of OLE loaded zein fibers is left undegraded. Fifty percent of initial phenolic content loaded into fibers is released which indicate the effect of OLE incorporation as accumulation of oleuropein. OLE-incorporated fibers immersed in PBS are less fused than pure zein fibers, due to the crosslinking effect.

Zein‐based composites in biomedical applications

“…The amino acid composition is a significant factor in determining the solubility of zein and accordingly, it is classified as α, β, γ, and δ zein . Zein as a biocompatible polymer has been proposed for applications in food packaging, textile fibers, coatings, but also in the biomedical and pharmaceutical fields . Paliwal and Palakurthi have reviewed applications of zein for drug delivery and tissue engineering.…”

“…17 Zein as a biocompatible polymer has been proposed for applications in food packaging, 18,19 textile fibers, 20 coatings, 21 but also in the biomedical and pharmaceutical fields. [22][23][24][25] Paliwal and Palakurthi 26 have reviewed applications of zein for drug delivery and tissue engineering. The present review covers the main properties of zein as a biomaterial with focus on zein-based composite materials, in particular zein combined with inorganic phases, for tissue engineering applications.…”

Silk fibroin nanofibers loaded with hydroxytyrosol from hydrolysis of oleuropein in olive leaf extract

“…In another study, oleuropein in OLE has been introduced as a natural, non-toxic cross linker for electrospun zein fi bres. Homogeneous fi bre morphology detected with alterati ons in bond structure in FTIR was att ributed to the eff ects of crosslinking eff ect of oleuropein [3].…”

“…The electrospinning method can be used to prepare nanofibers with a wide varieties of biopolymers including collagen, chitin, chitosan, cellulose, silk fibroin, hyaluronic acid, and zein [3]. Besides, with the addition of some additives or natural compounds into the structure of nanofiber mats, unique and tailor made properties can be acquired [4].…”

Adsorption/desorption and biofunctional properties of oleuropein loaded on different types of silk fibroin matrices

“…Among these techniques, electrospinning is usually preferred as it allows versatility in the control of the size of nanofibers, convenience for scaleup, and flexibility in terms of the materials used for nanofiber production [5]. Different biopolymers, such as collagen, silk fibroin, hyaluronic acid, and zein can be used to prepare nanofibers by electrospinning [2,6,7]. The silk fibroin used in this study is an important natural biopolymer and has been used to prepare electro-spun nanofibers that are used in many biotechnological applications such as in medical textiles, drug delivery and tissue scaffolding [8].…”