Oligotrophic Nitrification and Denitrification Bacterial Communities in a Constructed Sewage Treatment Ecosystem and Nitrogen Removal of Delftia tsuruhatensis NF4

Abstract

Oligotrophic nitrifiers and denitrifiers play important roles in the removal of nitrogen from wastewater. Here, we studied the dominant bacterial populations of the sewage treatment ecosystem (STE) water from different processes and those of culture on oligotrophic heterotrophic nitrification (OHN) medium and oligotrophic aerobic denitrification (OAD) medium, using co-analysis of Illumina HiSeq DNA sequencing and traditional culture methods. The results showed that the STE water had no dominant population of oligotrophic nitrifiers or oligotrophic denitrifiers. However, after culturing on OHN medium and OAD medium, the core genera Pseudomonas, Aeromonas, and Acinetobacter that have the nitrogen removal capacity in oligotrophic environments, dominated in the bacterial community. The principal component analysis (PCA) showed that the bacterial community in the constructed rapid infiltration (CRI) effluent water of STE had high similarity with those of cultures on OHN medium and OAD medium, which prompt the special purification role of nitrogen in the CRI system. The sodium alginate immobilized OAD bacteria strain Delftia tsuruhatensis NF4 was isolated from the CRI system, with total nitrogen (TN) removal efficiency of 43.3% in sterilized STE influent water, and 60.1% in OAD medium on day three. The immobilization significantly influenced the TN and nitrate removal efficiency in OAD medium (p < 0.05), but not in sterilized STE influent water (p > 0.05). This study would lay the foundation for resource discovery of oligotrophic heterotrophic nitrifiers and aerobic denitrifiers in STE and further functional application of them on the bioremediation of wastewater.

Halotolerant Endophytic Bacterium Serratia rubidaea ED1 Enhances Phosphate Solubilization and Promotes Seed Germination

“…Next, we showed that S. rubidaea ED1 grew on a N-free solid medium supporting its oligonitrophilic feature (Table 1) as previously reported in Serratia liquefaciens and Serratia marcescens [81,82]. Indeed, oligonitrophilic bacteria have broad substrate specificities that allow them to maintain their structure in low N environments [83]. However, other environmental factors, such as soil pH, could greatly influence the oligonitrophilic function [84].…”

Waste Polyurethane Foams as Biomass Carriers in the Treatment Process of Domestic Sewage with Increased Ammonium Nitrogen Content

“…The second most frequent group in our study belonged to the family Xanthomonadaceae , but these bacteria were much more prevalent in sample B (12.43%), whereas in sample A their share was 4.70% and it was smaller than that of genus Burkholderia (5.88%) and family Rhodospirillaceae (5.35%). Members of Xanthomonadales were also found by Yang et al [ 47 ] in the experimental sewage treatment ecosystem in relative abundances ranging from 10.5 to even 78%. These are bacteria capable of performing heterotrophic denitrification, similar to the members of Burkholderiales [ 41 ], which were also found by Yang et al [ 47 ] among the top three most abundant populations in one of the samples derived from experimental sewage treatment system with a relative abundance of 9.9%.…”

“…Members of Xanthomonadales were also found by Yang et al [ 47 ] in the experimental sewage treatment ecosystem in relative abundances ranging from 10.5 to even 78%. These are bacteria capable of performing heterotrophic denitrification, similar to the members of Burkholderiales [ 41 ], which were also found by Yang et al [ 47 ] among the top three most abundant populations in one of the samples derived from experimental sewage treatment system with a relative abundance of 9.9%. This group includes Burkholderia sp., which was on the other hand the most abundant genus in sample A and prevailed over their abundance in sample B (24.11% vs. 8.69%; Figure 8 ).…”

“…Members of Rhizobiaceae have been studied for their nitrification and aerobic denitrification capabilities and they might play important role in sewage treatment processes. Members of the family Rhizobiaceae and the order Rhizobiales were found to be among the top three relatively abundant bacterial populations in experimental sewage treatment ecosystem samples, with relative abundance ranging from 4.9% to even 60.5% [ 47 ]. The second most frequent group in our study belonged to the family Xanthomonadaceae , but these bacteria were much more prevalent in sample B (12.43%), whereas in sample A their share was 4.70% and it was smaller than that of genus Burkholderia (5.88%) and family Rhodospirillaceae (5.35%).…”

Assessing Microbial Diversity in Soil Samples Along the Potomac River: Implications for Environmental Health

“…The copyright holder for this preprint (which this version posted April 1, 2023. ; https://doi.org/10.1101/2023.03.31.535185 doi: bioRxiv preprint halophilic and thermophilic environments, and their presence suggests that the Potomac river basin could be facing increased hydrological stress. Chloroflexi are known to be involved in organohalide respiration and has potential roles in the bioremediation of chlorinated compounds 24 -noted due to historic PCB pollution in the Chesapeake Bay region. [25][26][27][28] As these bacteria are able to survive in extreme conditions, this could mean that the river basin is subject to increased levels of pollution and other environmental stressors.…”