Oligosaccharides obtained by partial hydrolysis of lipopolysaccharide from Fusobacterium nucleatum F1

Hofstad, Tor; Aadnegard, B.

doi:10.1111/j.1600-0765.1986.tb01448.x

Cited by 3 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LPS from oral strains of F. nucleatum consist of a typical lipid A component, exhibiting a close structural relationship to that of other groups of gram-negative bacteria (123), and an O-antigen heteropolysaccharide (133,174) and resemble LPS of other gram-negative bacteria (60). The LPS of F. nucleatum contain 3-deoxy-D-manno-octulosonic acid (KDO) (84,131,132). F. nucleatum strains have been classified into six chemotypes on the basis of the polysaccharide composition of their LPS (84).…”

Section: F Nucleatummentioning

confidence: 99%

Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum.

Bolstad

Jensen

Bakken

1996

Clinical Microbiology Reviews

115

147

View full text Add to dashboard Cite

Section: F Nucleatummentioning

confidence: 99%

Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum.

Bolstad

Jensen

Bakken

1996

Clinical Microbiology Reviews

115

147

View full text Add to dashboard Cite

“…The LPS of F. nucleatum contain 3-deoxy-D-manno-octulosonic acid (KDO) (84,131,132). F. nucleatum strains have been classified into six chemotypes on the basis of the polysaccharide composition of their LPS (84).…”

Section: F Nucleatummentioning

confidence: 99%

Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum

1996

View full text Add to dashboard Cite

The pathogenic potential of Fusobacterium nucleatum and its significance in the development of periodontal diseases, as well as in infections in other organs, have gained new interest for several reasons. First, this bacterium has the potential to be pathogenic because of its number and frequency in periodontal lesions, its production of tissue irritants, its synergism with other bacteria in mixed infections, and its ability to form aggregates with other suspected pathogens in periodontal disease and thus act as a bridge between early and late colonizers on the tooth surface. Second, of the microbial species that are statistically associated with periodontal disease, F. nucleatum is the most common in clinical infections of other body sites. Third, during the past few years, new techniques have made it possible to obtain more information about F. nucleatum on the genetic level, thereby also gaining better knowledge of the structure and functions of the outer membrane proteins (OMPs). OMPs are of great interest with respect to coaggregation, cell nutrition, and antibiotic susceptibility. This review covers what is known to date about F. nucleatum in general, such as taxonomy and biology, with special emphasis on its pathogenic potential. Its possible relationship to other periodontal bacteria in the development of periodontal diseases and the possible roles played by OMPs are considered.

show abstract

“…The amount of preparations of other bacterial strains separated on SDS-PAGE were visually adjusted after Coomassie brilliant blue staining according to the amount of the F. nucleatum Fevl sample. The extraction of LPS from F. nucleatum Fevl whole cells with phenol [23] and treatment with pronase, DNAase and RNAase was as described [24]. This preparation contained less than 1% amino acids with reference to the amount of glucosamine, as judged from amino-acid analysis of acid hydrolysates [22].…”

Section: Other Methodsmentioning

confidence: 99%

Outer membrane proteins as major antigens of Fusobacterium nucleatum

Bakken

Aarø

Hofstad

et al. 1989

FEMS Microbiology Letters

View full text Add to dashboard Cite

The immunochemical reactions of rabbit polyclonal antibodies directed to different preparations of Fusobacterium nucleatum i.e, whole cells, peptidoglycan associated proteins, a peptidoglycan‐protein complex and a purified 40 kiloDalton (kDa) protein, were investigated on outer membrane preparations of Fusobacterium species and a restricted number of Leptotrichia buccalis after their separation on sodium dodecyl sulphate polyacrylamide gels and electrotransfer to nitrocellulose. All F. nucleatum strains had identical reaction patterns with the immune sera tested. Surface exposed parts of a restricted number of proteins with apparent molecular weights at 70 kDa (a doublet band), 60 kDa, 55 kDa and 40 kDa seemed to be major immunogens. Antigenic related proteins either of identical or slightly deviating electrophoretic mobilities to the 40‐kDa protein were observed with the other members of Bacteroidaceae tested. The characteristic 70‐kDa protein doublet seemed to be restricted to F. nucleatum although single protein bands of near identical molecular weights belonging to the other species tested also reacted. The data also indicate that the 60‐kDa and 55‐kDa polypeptides might be present in other species of Fusobacterium.

show abstract

Oligosaccharides obtained by partial hydrolysis of lipopolysaccharide from Fusobacterium nucleatum F1

Cited by 3 publications

References 18 publications

Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum.

Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum.

Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum

Outer membrane proteins as major antigens of Fusobacterium nucleatum

Contact Info

Product

Resources

About