Oligonucleotide-Stabilized Ag Nanocluster Fluorophores

Richards, Christopher J.; Choi, Sungmoon; Hsiang, Jung-Cheng; Antoku, Yasuko; Vosch, Tom; Bongiorno, Angelo; Tzeng, Yih-Ling; Dickson, Robert M.

doi:10.1021/ja8005644

Cited by 820 publications

(889 citation statements)

References 20 publications

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“…It was soon demonstrated that different AgNC emission colors could be obtained by simply changing the sequence of DNA [35][36][37][38]. In 2006, Dickson and co-workers screened a diverse range of DNA sequences using a DNA microarray and identified various sequences that produce blue to near IR emitters ( Figure 1B) [39]. In addition, the same AgNC might emit different colors in different local environments.…”

Section: Synthesis Of Ncsmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

194

141

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In the past decade, fluorescent silver, gold and copper nanoclusters (Ag, Au and CuNCs) have emerged as a new class of signaling moiety for biosensor development. Compared to semiconductor quantum dots, metal NCs have less toxicity concerns and can be more easily conjugated to biopolymers. Due to their extremely small size, these NCs need a stabilizing ligand. Many polymers, proteins and nucleic acids have been reported to stabilize NCs. In particular, many DNA sequences produce highly fluorescence NCs. Coupling these DNA stabilizers with other sequences such as aptamers has generated a large number of biosensors. In this review, the synthesis of DNA and nucleotide-templated NCs is first summarized; their chemical interactions are also discussed. In the second part, the properties of NCs such as fluorescence quantum yield, emission wavelength and lifetime, structure and photostability are briefly reviewed. In the last part, various sensor design strategies using these NCs are categorized into the following four classes: 1) fluorescence de-quenching; 2) generation of templating DNA sequences to produce NCs; 3) change of nearby environment; and 4) reacting with heavy metal ions or other quenchers. Finally, the future trends in this field are discussed.

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Section: Synthesis Of Ncsmentioning

confidence: 99%

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Liu

2014

TrAC Trends in Analytical Chemistry

194

141

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“…We can broadly classify luminescent Ag NCs that have been successfully synthesized into two types: (1) macromoleculeprotected luminescent Ag NCs (for example, polymers, [37][38][39] dendrimers, 40 DNA [41][42][43] and proteins 44,45 ) and (2) thiol-protected luminescent Ag NCs. [46][47][48][49][50][51][52] Among the luminescent Ag NCs, thiolprotected Ag NCs are more attractive for biomedical applications, especially for subcellular imaging, because of their ultrasmall hydrodynamic diameters (o3 nm), facile post-functionalization and good stability.…”

Section: Introductionmentioning

confidence: 99%

Highly luminescent silver nanoclusters with tunable emissions: cyclic reduction–decomposition synthesis and antimicrobial properties

et al. 2013

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This paper reports a facile aqueous-based synthesis method for highly luminescent Ag nanoclusters (NCs) with tunable emissions. The key strategy was to use a reduction-decomposition-reduction cycle to modify the Ag NC intermediates that were further subjected to size/structure focusing. The as-modified Ag NC intermediates were more robust in water against the subsequent etching by thiol ligands, making a mild size-/structure-focusing environment possible, which produced highly luminescent Ag NCs with a well-defined size and structure. Ag NCs with intense red and green emission were successfully synthesized by this method. These Ag NCs possessed a well-defined size and structure (Ag 16 (SG) 9 and Ag 9 (SG) 6 ), and exhibited excellent stability in the aqueous phase. Our highly luminescent Ag NCs also possessed superior antimicrobial properties against the multidrug-resistant bacteria Pseudomonas aeruginosa, via generating a high concentration of intracellular reactive oxygen species. Keywords: antimicrobial; biomedical applications; luminescence; silver nanoclusters; thiol ligands INTRODUCTION Ultrasmall noble metal nanoclusters (NCs), typically composed of several to a hundred metal atoms, present unique physical and chemical properties between single atoms and large nanocrystals (42 nm). [1][2][3][4] Owing to the strong quantum size confinement in this size range (o 2 nm), NCs possess discrete and size-tunable electronic transitions and display unique molecule-like properties, such as quantized charging and luminescence. [5][6][7][8][9][10][11][12] Strong luminescence is one of the most attractive features of these NCs owing to their ultrafine size, good photostability and low toxicity; 13 some of these properties may not be realized by other luminophores, such as organic dyes (for example, photostability concerns) and semiconductor quantum dots (for example, relatively large size and toxicity concerns). 2,8,9,14,15 Recent studies have shown that luminescent Au and Ag NCs are promising optical probes for bioimaging and biosensing applications. 4,[16][17][18][19][20] This finding has led to the development of various methods for the synthesis of highly luminescent Au and Ag NCs. 2,21 On the other hand, insofar as nanostructured Ag-based materials are concerned, they are finding an increasing usage for antimicrobial applications owing to the unique chemistry of Ag interfacing with microorganisms. [22][23][24] Although there is a large volume of work exploring the potential use of large Ag

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“…In this case, we focus on the nearinfrared emitters using 633 nm as the excitation wavelength. Although a number of single molecule studies have been performed on AgNC to investigate specifi c photophysical parameters, [ 5,6,8,10,14,25,[27][28][29][30][31][32][33] we simultaneously measure several photophysical properties for each individual cluster, including decay times, emission spectra, and antibunching. This allows us to compare and correlate these photophysical properties within a sample to better understand the photophysical behavior and heterogeneity of these complex as-synthesized AgNC samples.…”

Section: Introductionmentioning

confidence: 99%

Single‐Molecule Characterization of Near‐Infrared‐Emitting Silver Nanoclusters

Hooley

Paolucci

Liao

et al. 2015

Advanced Optical Materials

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In this paper, single molecule spectroscopy is used to probe the photophysical heterogeneity of near‐infrared emitting silver nanoclusters stabilized in single stranded DNA containing 24 cytosines (C24‐AgNCs). Focusing on this subset of emitters allows us to address and correlate their photophysical parameters. The results suggest that for these near‐infrared emitting C24‐AgNCs, the broad range of observed photophysical properties is due to the spectral heterogeneity of two species, one of which has a broad range of emission maxima and decay times. This conclusion is drawn by analyzing the results of a large number of single C24‐AgNCs where, for the first time, emission maxima, fluorescence decay times, fluorescence intensity, blinking, and photon antibunching are determined simultaneously. The single molecule measurements also reveal that some of the C24‐AgNCs can change their spectral properties during the measurement, while photon antibunching measurements verified that all the analyzed C24‐AgNCs behave as single emitters. While the overall spectral heterogeneity can be related to the immobilization in the polymer film and/or intrinsic heterogeneity of the C24‐AgNCs emitters, the exact origin of the dynamical changes has not been fully determined. However, changes in the AgNCs geometry, local environment, or changes in the DNA conformation are possible explanations.

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Oligonucleotide-Stabilized Ag Nanocluster Fluorophores

Cited by 820 publications

References 20 publications

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

DNA-stabilized, fluorescent, metal nanoclusters for biosensor development

Highly luminescent silver nanoclusters with tunable emissions: cyclic reduction–decomposition synthesis and antimicrobial properties

Single‐Molecule Characterization of Near‐Infrared‐Emitting Silver Nanoclusters

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