Oligonucleotide microarray analysis of ameloblastoma compared with dentigerous cyst

Lim, Ji-Jun; Ahn, Hyo‐Won; Min, Sun-Hong; Hong, Seong‐Doo; Lee, Jae‐Il; Hong, Sam-Pyo

doi:10.1111/j.1600-0714.2006.00393.x

Cited by 29 publications

(22 citation statements)

References 39 publications

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“…This study focused on the description of the gene expression of malignant odontogenic tumors; we did not find any of those genes significantly expressed in our samples. Lim et al, in 2006, reported an oligonucleotide microarray analysis of 2 ameloblastomas compared with a dentigerous cyst (Lim et al, 2006). Overexpressed genes postulated to play a role in the tumorigenesis of ameloblastoma included VAV3, EGFR, TOM1L1, FUBP1, CDK2, TNKS, PTHRP, SMARCA2, KLK5, and CLU.…”

Section: Discussionmentioning

confidence: 99%

“…There have been two microarray studies of ameloblastoma (Heikinheimo et al, 2002;Carinci et al, 2004), one of them using cDNA microarrays containing 19,000 human cDNAs, and, more recently, an oligonucleotide analysis of whole-tissue ameloblastoma in a 34,000 human RNA microarray (Lim et al, 2006). The objectives of our study were: (a) to characterize the gene expression profile of laser-captured microdissected ameloblastoma cells using oligonucleotide microarray technology; (b) to compare the gene expression profile with universal human reference RNA; and (c) to identify genes or gene products that may have diagnostic, prognostic, or therapeutic potential.…”

Section: Introductionmentioning

confidence: 99%

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Microgenomics of Ameloblastoma

DeVilliers

Suggs²,

Simmons³

et al. 2011

J Dent Res

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A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. ABSTRACT Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microgenomics of Ameloblastoma

DeVilliers

Suggs²,

Simmons³

et al. 2011

J Dent Res

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“…Therefore, it is necessary to further elucidate other effects of COX-2 in the epithelium and connective tissue of odontogenic lesions and to compare dentigerous cyst with ameloblastoma. Previous studies have shown that each of ameloblastoma and dentigerous cyst presents a distinct evolution and biological behavior, and in addition to their clinical and behavioral differences (57), they reflect diversities in various features such as gene expression (58), pattern of expression of cytokines (3), interaction of different proteins, and collagen components (57).…”

Section: A B C D E F G Hmentioning

confidence: 99%

Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma

Alsaegh

Matsumoto

Taniguchi

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Investigation of cyclooxygenase (COX)-2 in dentigerous cyst and ameloblastoma may help to improve understanding of the nature and behavior of odontogenic cysts and tumors, and in addition may eventually represent a definitive target for a pharmacological approach in the management of these lesions. The aim of this study was to evaluate COX-2 expression and its correlation with the proliferation of odontogenic epithelium in these lesions. Dentigerous cysts (n=16) and ameloblastomas (n=17) were evaluated. Detection of Ki-67 and COX-2 protein expression was conducted by immunohistochemistry. Data were statistically analyzed using Mann-Whitney U test and Spearman's rank correlation coefficient. No significant differences were found in the expression of Ki-67 and COX-2 between dentigerous cysts and ameloblastomas (P>0.05). A significant positive correlation (P= 0.018) and highly significant positive correlation (P=0.004) were found between Ki-67 and COX-2 expression in the odontogenic epithelium of dentigerous cyst and ameloblastoma, respectively. COX-2 was expressed in the odontogenic epithelium of dentigerous cyst and ameloblastoma. It may contribute to local extension of these lesions by increasing the proliferation of their odontogenic epithelial cells.

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“…Expression of RB, the most important cell cycle regulator, has been lower in ameloblastomas than in dentigerous cysts, but higher in ameloblastic tumors than in tooth germs (58)(59). Cyclin D1 is strongly implicated in cell cycle progression by regulating the transition from G1 to S phase, while CKIs, p16…”

Section: Cell Cycle Regulatorsmentioning

confidence: 99%

Molecular alterations in the development and progression of odontogenic tumors

Kumamoto

2010

Oral Med Pathol

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Odontogenic tumors are lesions derived from the elements of the tooth-forming apparatus and are found exclusively within the jawbones. This review presents a contemporary outline of our current understanding of the molecular and genetic alterations associated with the development and progression of odontogenic tumors, including apoptosis-related factors, telomerase, cell cycle regulators, growth factors, regulators of tooth development, hard tissue-related proteins, cell adhesion molecules, matrix-degrading proteinases, angiogenic factors, and osteolytic cytokines. It is hoped that better understanding of related molecular mechanisms will help to predict the course of odontogenic tumors and lead to the development of new therapeutic concepts for their management.

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Oligonucleotide microarray analysis of ameloblastoma compared with dentigerous cyst

Abstract: We identified important genes related to the development and progression of ameloblastoma through a large-scale gene expression analysis. This study will stimulate further investigations on genes significant for early diagnosis and prognosis of ameloblastoma.

Cited by 29 publications

References 39 publications

Microgenomics of Ameloblastoma

Microgenomics of Ameloblastoma

Odontogenic epithelial proliferation is correlated with COX-2 expression in dentigerous cyst and ameloblastoma

Molecular alterations in the development and progression of odontogenic tumors

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