Oligonucleotide IMT504 Improves Glucose Metabolism and Controls Immune Cell Mediators in Female Diabetic NOD Mice

IMT504 blocks allodynia in rats with spared nerve injury by promoting the migration of mesenchymal stem cells and by favoring an anti-inflammatory milieu at the injured nerve

“…Some experimental groups were injected using a 24-mer PolyC ODN (6 mg/kg) with no immune stimulating capacity, as an additional control. 3…”

IMT504 protects beta cells against apoptosis and maintains beta cell identity, without modifying proliferation

“…With this in mind, and since IMT504 has both immunomodulatory and regenerative properties, our group started to evaluate this ODN as a possible therapy for T1D in several animal models, finding very promising results in terms of beta cell recovery, improvement on glucose and insulin blood levels, and decrease in beta cell apoptosis, and insulitis and infiltration of CD45+ leukocytes into the pancreas (Bianchi et al, 2010(Bianchi et al, , 2012(Bianchi et al, , 2016(Bianchi et al, , 2021. Nevertheless, these in vivo experiments did not hint to a possible direct effect of IMT504 on βcells.…”

“…IMT504 promoted marked improvement in rat models of bone injury (Hernando et al, 2007), neuropathic pain (Coronel et al, 2008), and sepsis (Chahin et al, 2015). Regarding diabetes, we have previously demonstrated that IMT504 promotes significant improvement in the diabetic condition in various animal models: For example, induction of βcell recovery, inhibition of leukocyte infiltration into the islet, improvement on glucose and insulin blood levels, decrease in apoptosis, and insulitis and infiltration of CD45+ leukocytes into the pancreas (Bianchi et al, 2010(Bianchi et al, , 2012(Bianchi et al, , 2016(Bianchi et al, , 2021. TLR-9 is the receptor that recognizes DNA oligonucleotides from the CpG ODN class.…”

“…Thus, therapeutic approaches designed to regulate apoptosis could be a significant new development in the management of diabetes. Bianchi et al demonstrated that in diabetic animals the immunomodulatory ODN IMT504 induced a decrease in βcell apoptosis (Bianchi et al, 2016(Bianchi et al, , 2021.…”

Sexual dimorphism modulates metabolic and cognitive alterations under HFD nutrition and chronic stress exposure in mice. Correlation between spatial memory impairment and BDNF mRNA expression in hippocampus and spleen

“…Serum insulin concentration was determined by a radioimmunoassay (RIA) using humanized porcine insulin for iodination and standard, provided by Laboratorios Beta J o u r n a l P r e -p r o o f 9 (Buenos Aires, Argentina), and anti-bovine/ human/ pig insulin antibody (Sigma Cat N° I8510) (Bianchi et al, 2021;García-Tornadú et al, 2010). Minimum detectable concentrations were 0.08 ng and intra-and inter-assay coefficients of variation were 7.2 ± 1.1 and 11.4 ± 1.5 % respectively.…”