2010
DOI: 10.1134/s1068162010060105
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Oligonucleotide derivatives in the hybridization analysis of nucleic acids: II. Isothermal signal amplification in DNA analysis by minisequencing

Abstract: The isothermal amplification of reporter signal via limited probe extension (minisequencing) upon hybridization of nucleic acids has been studied. The intensity of reporter signal has been shown to increase due to enzymatic labeling of multiple probes upon consecutive hybridization with one DNA template both in homophase and heterophase assays using various kinds of detection signal: radioisotope label, fluorescent label, and enzyme-linked assay. The kinetic scheme of the process has been proposed and kinetic … Show more

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Cited by 2 publications
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“…The subsequent hybridization with complementary ss-DNA sequences (target strand) has been detected by various analytical techniques based on gravimetric [1,2], radioisotope or fluorescence labelling [3,4], surface plasmon resonance [5] or quartz crystal microbalance [6].…”
Section: Introductionmentioning
confidence: 99%
“…The subsequent hybridization with complementary ss-DNA sequences (target strand) has been detected by various analytical techniques based on gravimetric [1,2], radioisotope or fluorescence labelling [3,4], surface plasmon resonance [5] or quartz crystal microbalance [6].…”
Section: Introductionmentioning
confidence: 99%