Oligonucleotide‐Based Photoaffinity Probes: Chemical Tools and Applications for Protein Labeling
Carole Saintomé,
Océane Monfret,
Gilles Doisneau
et al.
Abstract:A variety of proteins interact with DNA and RNA, including polymerases, histones, ribosomes, transcription factors, and repair enzymes. However, the transient non‐covalent nature of these interactions poses challenges for analysis. Introducing a covalent bond between proteins and DNA via photochemical activation of a photosensitive functional group introduced onto nucleic acids offers a means to stabilize these often weak interactions without significantly altering the binding interface. Consequently, photoact… Show more
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