Oligomerization of G‐Protein‐Coupled Receptors

López-Giménez, Juan F.; González-Maeso, Javier

doi:10.1002/9781118185537.ch5

Cited by 1 publication

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“…Conversely, the laminar distribution of porcine 5-HT 2A receptors in cortical areas (Fig. 2) resembles that of humans and nonhuman primates with the highest density of 5-HT 2A receptors superficially located (Hall et al, 2000;Lopez-Gimenez et al, 1998 in contrast to the rat brain, where the highest density is found in the Differences between treatment groups are evaluated vs. saline groups using one-way ANOVA for each brain region and Tukey post-test for multiple comparisons: *p<0.001, **p<0.01, ***p<0.05.…”

Section: Discussionmentioning

confidence: 93%

An approach for serotonin depletion in pigs: Effects on serotonin receptor binding

Ettrup

Kornum

Weikop

et al. 2010

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Depletion of central serotonin (5-HT) levels and dysfunction in serotonergic transmission are implicated in a variety of human CNS disorders. The mechanisms behind these serotonergic deficits have been widely studied using rodent models, but only to a limited extent in larger animal models. The pig is increasingly used as an experimental animal model especially in neuroscience research. Here, we present an approach for serotonin depletion in the pig brain. Central serotonin depletion in Danish Landrace pigs was achieved following 4 days treatment with para-chlorophenylalanine (pCPA). On day 5, tissue concentrations of 5-HT in seven distinct brain structures from one hemisphere: frontal and occipital cortex, striatum, hippocampus, cerebellum, rostral, and caudal brain stem, were determined. The other hemisphere was processed for receptor autoradiography. Treatments with 50 mg/kg and 100 mg/kg pCPA caused average decreases in 5-HT concentrations of 61% ± 14% and 66% ± 16%, respectively, and a substantial loss of 5-HT immunostaining was seen throughout the brain. The serotonin depletion significantly increased 5-HT₄ receptor binding in nucleus accumbens, but did not alter 5-HT(1A) and 5-HT(2A) receptor or serotonin transporter binding in any brain region. In conclusion, 4 days treatment with pCPA effectively reduces 5-HT levels in the pig brain. Further, whereas several 5-HT markers did not change after the pCPA treatment, 5-HT₄ receptors were consistently upregulated, indicating a greater susceptibility of this receptor to altered 5-HT levels. This porcine model of serotonin depletion will be useful in future studies of cerebral serotonergic dysfunction.

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Section: Discussionmentioning

confidence: 93%