Oligomeric Proanthocyanidins and Bamboo Leaf Flavonoids Improve the Quality of Bull Semen Cryopreservation

Abstract

It is important to inhibit oxidative stress to maintain sperm motility during cryopreservation. The present study was performed to investigate the effects of supplementing oligomeric proanthocyanidins (OPC) and bamboo leaf flavonoids (BLF) or their combination as an extender for Simmental bull semen freezing. OPC, BLF, or their combination were added to the frozen diluent of bovine semen. Afterwards, computer-assisted semen analysis (CASA), detection of membrane functionality, acrosome integrity, mitochondrial integrity, CAT, SOD, GSH-PX, MDA, and ROS were conducted. The results showed that adding 50 mg/L OPC or 4 mg/L BLF could improve the quality of frozen sperm. Compared with 50 mg/L OPC alone, the combination of 50mg/L OPC and 2 mg/L BLF significantly increased the kinematic parameters of sperm, and sperm CAT, GSH-PX and SOD levels (p < 0.05), whereas the MDA of sperm was decreased (p < 0.05). These results indicated that compared to the addition of 50 mg/L OPC alone, a combination of 50 mg/L OPC and 2 mg/L BLF could further improve the quality of frozen semen. The results could provide theoretical data support for the development of a new protective agent and are significant for the cryopreservation of bovine semen in the future.

Morin hydrate suppresses lipoteichoic acid-induced oxidative stress-mediated inflammatory events in macrophages via augmenting Nrf2/HO-1 and antioxidant defense molecules

“…This is evidenced by the observed increase in the activities and levels of CAT, SOD and GSH following MH treatment which has similar results to other flavonoids. 47 These findings support the notion that MH has the potential in regulating the imbalances in antioxidant enzyme induced by LTA, thereby contributing to cellular protection against oxidative stress.…”

Oligomeric Proanthocyanidins: An Updated Review of Their Natural Sources, Synthesis, and Potentials

“…The dimeric proanthocyanidins exhibit therapeutic ability in psoriasis, the result showed it can significantly relieve psoriasis symptoms after administration of dimeric proanthocyanidins to mice and the protein and mRNA levels of anti-oxidative stress (anti-OS) and anti-inflammatory biomarkers of the largest dose (50 milligrams per kilogram) group were higher than other groups [151]. In addition, the OPCs had a protective effect on sperm at low temperatures and are more effective when combined with chloro-flavonoids [152]. In the protection of teeth, the GSE group showed better results than another group, which contained 10% sodium ascorbate [153].…”

Efeito da adição do extrato de capim-santo (Cymbopogon citratus) sobre a refrigeração de espermatozoides epididimários bovinos

“…Considerando que a produção excessiva de espécies reativas de oxigênio (ERO) pode prejudicar a fertilidade e viabilidade espermática, a presença desses compostos no extrato de capim-santo sugere um potencial benéfico em diluidores espermáticos. Adicionalmente, os fitoesteróis presentes, similares ao colesterol nas células de mamíferos, destacam-se como elementos vitais nas membranas celulares, oferecendo uma alternativa viável para melhorar os parâmetros espermáticos e a fertilidade em diluidores (Liman et al, 2022;Rajoriya et al, 2020;Wang et al, 2022).…”