Oligogalacturonides inhibit the formation of roots on tobacco explants

Bellincampi, Daniela; Salvi, Giovanni E.; Lorenzo, Giulia De; Cervone, Felice; Marfâ, Victòria; Eberhard, Stefan; Darvill, Alan; Albersheim, Peter

doi:10.1046/j.1365-313x.1993.04010207.x

Cited by 90 publications

(76 citation statements)

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“…Furthermore, auxin-dependent responses are impaired in tobacco PG plants, and our previous work has shown that OGs suppress different responses regulated by auxin in tobacco (Bellincampi et al, 1993(Bellincampi et al, , 1996Mauro et al, 2002). In particular, tobacco PG plants show reduced sensitivity to IAA in experiments of rhizogenesis in leaf explants and inhibition of primary root growth.…”

Section: Discussionmentioning

confidence: 95%

“…Because OGs have auxin-antagonistic activity and treatment with OGs leads to a decreased sensitivity to auxin in tobacco plants (Branca et al, 1988;Bellincampi et al, 1993Bellincampi et al, , 1996Altamura et al, 1998;Mauro et al, 2002), a support for the possible involvement of OGs in the phenotype of PG plants might be provided by the ability of auxin to revert some of the phenotypic features as well as by a decreased sensitivity to auxin of the PG plants.…”

Section: Increased Resistance Of Tobacco Pg Plants Is Abolished By Auxinmentioning

confidence: 99%

“…In particular, a number of reports from our laboratory indicate that exogenously added OGs are able to antagonize the action of auxin, as in the case of pea (Pisum sativum) stem elongation (Branca et al, 1988), tobacco (Nicotiana tabacum) adventitious root formation (Bellincampi et al, 1993), and pericycle cell differentiation (Altamura et al, 1998). OGs prevent rhizogenesis in tobacco leaf explants expressing the Agrobacterium tumefaciens rolB gene by inhibiting the auxin-induced expression of the transgene (Bellincampi et al, 1996).…”

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Transgenic Expression of a Fungal endo-Polygalacturonase Increases Plant Resistance to Pathogens and Reduces Auxin Sensitivity

et al. 2007

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Polygalacturonases (PGs), enzymes that hydrolyze the homogalacturonan of the plant cell wall, are virulence factors of several phytopathogenic fungi and bacteria. On the other hand, PGs may activate defense responses by releasing oligogalacturonides (OGs) perceived by the plant cell as host-associated molecular patterns. Tobacco (Nicotiana tabacum) and Arabidopsis (Arabidopsis thaliana) plants expressing a fungal PG (PG plants) have a reduced content of homogalacturonan. Here, we show that PG plants are more resistant to microbial pathogens and have constitutively activated defense responses. Interestingly, either in tobacco PG or wild-type plants treated with OGs, resistance to fungal infection is suppressed by exogenous auxin, whereas sensitivity to auxin of PG plants is reduced in different bioassays. The altered plant defense responses and auxin sensitivity in PG plants may reflect an increased accumulation of OGs and subsequent antagonism of auxin action. Alternatively, it may be a consequence of perturbations of cellular physiology and elevated defense status as a result of altered cell wall architecture.

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Section: Discussionmentioning

confidence: 95%

Section: Increased Resistance Of Tobacco Pg Plants Is Abolished By Auxinmentioning

confidence: 99%

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confidence: 99%

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Transgenic Expression of a Fungal endo-Polygalacturonase Increases Plant Resistance to Pathogens and Reduces Auxin Sensitivity

et al. 2007

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“…Meristem formation is triggered by a 10 3 -to 10 4 -fold increase in sensitivity to auxin of plant cells (Spanò et al, 1988;Maurel et al, 1991), possibly due to the tyrosine phosphatase activity of the RolB protein (Filippini et al, 1996), which may perturb the auxin signal transduction pathway. Oligogalacturonides, a class of oligosaccharins with antiauxin effects (Darvill et al, 1992;Bellincampi et al, 1993), inhibit auxin induction of rolB (Bellincampi et al, 1996).…”

Section: Ntbbf1 and Rolb Auxin Inductionmentioning

confidence: 99%

The DNA Binding Site of the Dof Protein NtBBF1 Is Essential for Tissue-Specific and Auxin-Regulated Expression of the rolB Oncogene in Plants

et al. 1999

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The Dof proteins are a large family of plant transcription factors that share a single highly conserved zinc finger. The tobacco Dof protein NtBBF1 was identified by its ability to bind to regulatory domain B in the promoter of the rolB oncogene. In this study, we show that the ACT TTA target sequence of NtBBF1 in domain B is necessary for tissue-specific expression of rolB . ␤ -Glucuronidase (GUS) activity of tobacco plants containing a rolB promoter-GUS fusion with a mutated NtBBF1 target sequence within domain B is almost completely suppressed in apical meristems and is severely abated in the vascular system. The ACTTTA motif is shown here also to be one of the cis -regulatory elements involved in auxin induction of rolB . The pattern of NtBBF1 expression in plants is remarkably similar to that of rolB , except in mesophyll cells of mature leaves, in which only NtBBF1 expression could be detected. Ectopic expression of rolB in mesophyll cells was achieved by particle gun delivery if the NtBBF1 binding sequence was intact. These data provide evidence that in the plant, a Dof protein DNA binding sequence acts as a transcriptional regulatory motif, and they point to NtBBF1 as the protein involved in mediating tissue-specific and auxin-inducible expression of rolB . INTRODUCTIONInfection of dicotyledonous plants with Agrobacterium rhizogenes results in hairy root disease (Elliot, 1951). Hairy root causes an abundant proliferation of neoplastic roots containing T-DNA from the root-inducing (Ri) bacterial plasmid (Chilton et al., 1982;Spanò et al., 1982;White et al., 1982;Willmitzer et al., 1982). In the T-DNA sequence, 18 open reading frames have been identified (Slightom et al., 1986). Four of these, termed the rol oncogenes, coincide with genetic loci previously localized by transposon mutagenesis (White et al., 1985). Among the rol oncogenes, rolB is the only one that, when inactivated, totally suppresses root induction by A. rhizogenes (White et al., 1985). In addition, rolB is the only individual gene capable of inducing growth of transformed roots for all plants tested (Cardarelli et al., 1987;Spena et al., 1987;Capone et al., 1989). More recently, in cultured thin cell layers ( Tran Thanh Van et al., 1974), the stimulation of meristem formation has been shown to be the primary effect of rolB (Altamura et al., 1994). Expression of rolB greatly increases the sensitivity of transformed plant cells to auxin (Spanò et al., 1988;Maurel et al., 1991), and tyrosine phosphatase activity has been associated with the RolB protein when it is expressed in Escherichia coli (Filippini et al., 1996), suggesting that RolB may act by perturbing the auxin signal transduction pathway.The expression of rolB in transgenic plants also has been extensively analyzed and found to be strongest in root and shoot meristems (Schmülling et al., 1989;Maurel et al., 1990;Altamura et al., 1991;Capone et al., 1991), which is in accord with an effect of the oncogene on meristem induction. Expression was also detectable in the vascular system....

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“…For example, flg22 and OGs generate an extracellular oxidative burst mediated by RESPIRATORY BURST OXIDASE HOMOLOG D (RbohD) and induce protection against the necrotrophic fungus Botrytis cinerea independently of the ethylene, jasmonic acid, and salicylic acid pathways and of the RbohD-mediated production of reactive oxygen species (ROS; Zhang et al, 2007;Galletti et al, 2008). The inhibition of auxin responses is another feature shared by PAMPs and DAMPs (Savatin et al, 2011); in the case of OGs, the inhibition of auxin responses has been described as a true antagonism (Branca et al, 1988;Bellincampi et al, 1993;Savatin et al, 2011). On the other hand, microarray analyses indicate that late responses to the two classes of elicitors are considerably different .…”

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The Arabidopsis NUCLEUS- AND PHRAGMOPLAST-LOCALIZED KINASE1-Related Protein Kinases Are Required for Elicitor-Induced Oxidative Burst and Immunity

et al. 2014

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Plant immunity is activated through complex and cross-talking transduction pathways that include a mitogen-activated protein kinase phosphorylation cascade. Here, we have investigated the role in immunity of the Arabidopsis (Arabidopsis thaliana) gene subfamily that encodes the mitogen-activated protein triple kinases indicated as ARABIDOPSIS NUCLEUS-AND PHRAGMOPLAST-LOCALIZED KINASE1-RELATED PROTEIN KINASE1 (ANP1), ANP2, and ANP3. For this study, we used representative danger signals (elicitors) belonging to the classes of the damage-and pathogen-associated molecular patterns, i.e. oligogalacturonides, linear fragments derived from the plant cell wall homogalacturonan, and the peptide elf18 derived from the bacterial elongation factor thermo-unstable. Analyses of single and double as well as conditional triple mutants show that ANPs are required for elicitor-triggered defense responses and protection against the necrotrophic fungus Botrytis cinerea. Notably, ANPs are also required for both the elicitor-induced oxidative burst and the transduction of the hydrogen peroxide signal but not for the inhibition of auxin-induced gene expression, indicating that this response can be uncoupled from the activation of defense responses. Our findings point to ANPs as key transduction elements that coordinate damage-and pathogen-associated molecular patterntriggered immunity and orchestrate reactive oxygen species accumulation and signaling.

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Oligogalacturonides inhibit the formation of roots on tobacco explants

Cited by 90 publications

References 0 publications

Transgenic Expression of a Fungal endo-Polygalacturonase Increases Plant Resistance to Pathogens and Reduces Auxin Sensitivity

Transgenic Expression of a Fungal endo-Polygalacturonase Increases Plant Resistance to Pathogens and Reduces Auxin Sensitivity

The DNA Binding Site of the Dof Protein NtBBF1 Is Essential for Tissue-Specific and Auxin-Regulated Expression of the rolB Oncogene in Plants

The Arabidopsis NUCLEUS- AND PHRAGMOPLAST-LOCALIZED KINASE1-Related Protein Kinases Are Required for Elicitor-Induced Oxidative Burst and Immunity

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