Oligodeoxynucleotide Analogues of Circulating DNA Inhibit dsRNA-Induced Immune Response at the Early Stages of Signal Transduction Cascade in a Cell Type-Dependent Manner

Abstract

Oligodeoxynucleotide (ODN) analogues of cell-surface-bound circulating DNA inhibit the dsRNA-induced production of pro-inflammatory interleukin 6, interferon beta and antibacterial peptide beta-defensin 2 not only in human gingival fibroblasts, but also in human primary endothelial and transformed cells (Hela and A431). ODN analogues do not effect dendritic cells activation by poly(I:C). The data obtained indicate that the early stages of the signal transduction cascade are violated by ODN analogues and the effects depend on the cell type.

Cell‐surface‐bound circulating DNA in the blood: Biology and clinical application

“…Nonetheless, we have observed when studying the effect of DNA on the inhibition of immune response induction by double‐strand RNA that csbDNA is a more efficient inhibitor for the proinflammatory cytokine production by fibroblasts and endothelial cells as compared with the genomic or circulating DNAs . Although we have detected immunomodulatory motifs in the csbDNA pool, the mechanism underlying the effect of csbDNA on the double‐strand RNA–induced immune response is still rather vague .…”