Oligodendroglial Expression of Edg-2 Receptor: Developmental Analysis and Pharmacological Responses to Lysophosphatidic Acid

Stankoff, Bruno; Barrón, Sonia; Allard, Julien; Barbin, G.; Noël, Frédéric; Aigrot, Marie‐Stéphane; Prémont, Joël; Sokoloff, Pierre; Zalc, Bernard; Lubetzki, Catherine

doi:10.1006/mcne.2002.1129

Cited by 40 publications

(45 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell surface receptors known to mediate LPA signaling are expressed by post-migratory, premyelinating oligodendrocytes (Weiner et al, 1998;Stankoff et al, 2002;Yu et al, 2004). Thus, PD-Iα/ATX's enzymatic activity likely contributes to oligodendrocyte development and/or function.…”

Section: Discussionmentioning

confidence: 99%

Phosphodiesterase-Iα/autotaxin's MORFO domain regulates oligodendroglial process network formation and focal adhesion organization

Dennis

White

Forrest

et al. 2008

Molecular and Cellular Neuroscience

View full text Add to dashboard Cite

Development of a complex process network by maturing oligodendrocytes is a critical but currently poorly characterized step toward myelination. Here, we demonstrate that the matricellular oligodendrocyte-derived protein phosphodiesterase-Iα/autotaxin (PD-Iα/ATX) and especially its MORFO domain are able to promote this developmental step. In particular, the single EF hand-like motif located within PD-Iα/ATX's MORFO domain was found to stimulate the outgrowth of higher order branches but not process elongation. This motif was also observed to be critical for the stimulatory effect of PD-Iα/ATX's MORFO domain on the reorganization of focal adhesions located at the leading edge of oligodendroglial protrusions. Collectively, our data suggest that PD-Iα/ATX promotes oligodendroglial process network formation and expansion via the cooperative action of multiple functional sites located within the MORFO domain and more specifically, a novel signaling pathway mediated by the single EF hand-like motif and regulating the correlated events of process outgrowth and focal adhesion organization.

show abstract

Section: Discussionmentioning

confidence: 99%

Phosphodiesterase-Iα/autotaxin's MORFO domain regulates oligodendroglial process network formation and focal adhesion organization

Dennis

White

Forrest

et al. 2008

Molecular and Cellular Neuroscience

View full text Add to dashboard Cite

show abstract

“…Alexa (488 or 594)-conjugated sheep anti-mouse IgG1 and IgG3 and Alexa-conjugated goat anti-mouse IgM, anti-rabbit IgG, and anti-rat IgG were from Molecular Probes (Eugene, OR) and used at a dilution of 1:1000. For Western blotting, we used the following dilutions: anti-phosphorylated Akt (mouse mAb IgG2b, 1:1000; Ozyme), anti-phosphorylated CRMP2 antibody (rabbit polyclonal, 1:50) (Matsui et al, 1996), rabbit anti-Edg2/ LPA1 antiserum (1:1000) (Stankoff et al, 2002b), and peroxidaseconjugated anti-mouse and anti-rabbit Ig (1:5000; Dako, Glostrup, Denmark). S1P was purchased from Sigma.…”

Section: Methodsmentioning

confidence: 99%

“…Edg2/LPA1 and Edg8/S1P5 mRNA are predominantly expressed in the CNS white matter (Allard et al, 1998;Im et al, 2001). At the cellular level, Edg2/ LPA1 has been shown to be expressed on mature oligodendrocytes (Allard et al, 1998;Cervera et al, 2002;Stankoff et al, 2002b), whereas its expression on immature oligodendrocytes remains controversial (Moller et al, 1999;Dawson et al, 2003). Immunohistochemical analysis has suggested that Edg8/S1P5 is expressed in NG2-positive OPCs but not in mature oligodendrocytes (Terai et al, 2003), whereas expression of Edg8/S1P5 transcripts has been detected by reverse transcription (RT)-PCR in differentiated rat oligodendrocytes in culture (Yu et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Edg8/S1P5: An Oligodendroglial Receptor with Dual Function on Process Retraction and Cell Survival

Jaillard

Harrison²,

Stankoff³

et al. 2005

J. Neurosci.

312

276

View full text Add to dashboard Cite

Endothelial differentiation gene (Edg) proteins are G-protein-coupled receptors activated by lysophospholipid mediators: sphingosine-1-phosphate (S1P) or lysophosphatidic acid. We show that in the CNS, expression of Edg8/S1P5, a high-affinity S1P receptor, is restricted to oligodendrocytes and expressed throughout development from the immature stages to the mature myelin-forming cell. S1P activation of Edg8/S1P5 on O4-positive pre-oligodendrocytes induced process retraction via a Rho kinase/collapsin response-mediated protein signaling pathway, whereas no retraction was elicited by S1P on these cells derived from Edg8/S1P5-deficient mice. Edg8/S1P5-mediated process retraction was restricted to immature cells and was no longer observed at later developmental stages. In contrast, S1P activation promoted the survival of mature oligodendrocytes but not of pre-oligodendrocytes. The S1P-induced survival of mature oligodendrocytes was mediated through a pertussis toxin-sensitive, Akt-dependent pathway. Our data demonstrate that Edg8/S1P5 activation on oligodendroglial cells modulates two distinct functional pathways mediating either process retraction or cell survival and that these effects depend on the developmental stage of the cell.

show abstract

“…Basally, Lpar1 colocalizes with proteolipid protein and myelin basic protein (MBP), but not glial fi brillary acidic protein (91,92). During development, Lpar1 expression in OLs emerges just prior to maturation/myelination, suggesting an important role in controlling these processes, although no effect of LPA on OL survival, maturation, myelination, or cytoskeleton organization was reported in vitro (129). However, both the rat OL precursor cell line CG-4 and freshly isolated precursors respond via Rho-ROCK cell rounding upon LPA exposure, in contrast to differentiated OLs (130).…”

Section: Lpa Receptorsmentioning

confidence: 99%

LPA receptor signaling: pharmacology, physiology, and pathophysiology

Yung

Stoddard

Chun

2014

Journal of Lipid Research

597

683

View full text Add to dashboard Cite

Oligodendroglial Expression of Edg-2 Receptor: Developmental Analysis and Pharmacological Responses to Lysophosphatidic Acid

Cited by 40 publications

References 66 publications

Phosphodiesterase-Iα/autotaxin's MORFO domain regulates oligodendroglial process network formation and focal adhesion organization

Phosphodiesterase-Iα/autotaxin's MORFO domain regulates oligodendroglial process network formation and focal adhesion organization

Edg8/S1P5: An Oligodendroglial Receptor with Dual Function on Process Retraction and Cell Survival

LPA receptor signaling: pharmacology, physiology, and pathophysiology

Contact Info

Product

Resources

About