Oligoclonal T cells are infiltrating the brains of children with AIDS: sequence analysis reveals high proportions of identical β-chain T-cell receptor transcripts

CD8+ Lymphocyte-Mediated Injury of Dorsal Root Ganglion Neurons during Lentivirus Infection: CD154-Dependent Cell Contact Neurotoxicity

“…These findings are consistent with previous reports that activated T cells could injure neurons in both syngeneic and allogeneic mechanisms in vitro (Manning et al, 1987;Rall et al, 1995;Medana et al, 2001a;Giuliani et al, 2003). HIV infection can also elicit autoreactive T cell responses in the circulation and in the brains of infected hosts (Lin et al, 2005); these findings resembled the marked influx and adverse effects of T cells in DRGs of FIV-infected animals, whereas the few resident T cells in DRGs of healthy cats did not exert deleterious effects on neurons. Soluble factors have also been demonstrated to contribute to DRG neuron injury (Nagano et al, 1996).…”

“…HIV-infected patients have circulating autoreactive cytotoxic T lymphocyte (CTL) and infiltrating CD8ϩ T cells in the brain, which recognize host and not viral peptides (Salemi et al, 1995;di Marzo Veronese et al, 1996;Miller et al, 2004;Lin et al, 2005). There are reports describing neurons that express MHC-I molecules (Rall et al, 1995;Abendroth et al, 2000), which are necessary for CTL recognition of target cells.…”

Aneurysmal Lesions of Patients with Abdominal Aortic Aneurysm Contain Clonally Expanded T Cells

“…This adaptor, a modification of the one previously described (32)(33)(34)(35)(36)(37), consists of two oligonucleotides (Supplemental Table I), the 59-AATTCGAACCCCTTCGAGAA-TGCG-39 and its complementary 39-GCTTGGGGAAGCTCTTACGC-p-59, preannealed to each other. The ligated adaptor was removed from the 39 end of the double-stranded cDNA by digestion with NotI restriction endonuclease, as described (32)(33)(34)(35)(36)(37), while it remained in the 59 end. The digested cDNA was purified using a G-50 column (59-39, Boulder, CO), as recommended by the manufacturer.…”

“…The blunt-ended cDNA was ligated at both the 59 and 39 blunt ends with an equivalent molar concentration of a nonpalindromic adaptor (NPA) (32)(33)(34)(35)(36)(37) by incubation for 14 h at 16˚C with T4 DNA ligase (Life Technologies-BRL). This adaptor, a modification of the one previously described (32)(33)(34)(35)(36)(37), consists of two oligonucleotides (Supplemental Table I), the 59-AATTCGAACCCCTTCGAGAA-TGCG-39 and its complementary 39-GCTTGGGGAAGCTCTTACGC-p-59, preannealed to each other. The ligated adaptor was removed from the 39 end of the double-stranded cDNA by digestion with NotI restriction endonuclease, as described (32)(33)(34)(35)(36)(37), while it remained in the 59 end.…”

“…cDNA was synthesized from total RNA and primed with oligo-(dT) 15 -NotI (Promega), using SuperScript II (Gibco/Brl), following the manufacturer's instructions (32)(33)(34)(35)(36)(37). Double-stranded cDNA was blunt ended for efficient adaptor ligation by adding T4 DNA polymerase.…”