Oligella, a New Genus Including Oligella urethralis comb. nov. (Formerly Moraxella urethralis) and Oligella ureolytica sp. nov. (Formerly CDC Group IVe): Relationship to Taylorella equigenitalis and Related Taxa

Assignment of Centers for Disease Control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov.

“…Later, Yabuuchi et al (1 995) reclassified Alcaligenes eutrophus, Pseudomonas solanacearum and Pseudomonas pickettii in a novel genus Ralstonia, as Ralstonia eutropha, Ralstonia solanacearum and Ralstonia pickettii, respectively. The T,(e) values of DNA of CDC group IVc-2 strains towards radioactively labelled rRNA of the R. eutropha type strain (Rossau et al, 1987) indicate that CDC group IVc-2 belongs to the same genus; this was recently confirmed by 16s rDNA gene sequence analysis as reported by Osterhout et al (1998). In the present study, we performed an integrated genotypic and phenotypic analysis of CDC group IVc-2 strains to characterize their phenotype and genotype, and their relatedness towards established species of the genus Ralstonia.…”

“…In 1987, Rossau and colleagues reported that CDC group IVc-2 strains belonged to rRNA superfamily I11 sensu De Ley or the / 3 subclass of the Proteobacteria (De Ley, 1992), with the generically misnamed Alcaligenes eutrophus and Pseudomonas solanacearum as its closest neighbours (Kersters & De Ley, 1984;Rossau et al, 1987). Later, Yabuuchi et al (1 995) reclassified Alcaligenes eutrophus, Pseudomonas solanacearum and Pseudomonas pickettii in a novel genus Ralstonia, as Ralstonia eutropha, Ralstonia solanacearum and Ralstonia pickettii, respectively.…”

Azoarcus gen. nov., Nitrogen-Fixing Proteobacteria Associated with Roots of Kallar Grass (Leptochloa fusca (L.) Kunth), and Description of Two Species, Azoarcus indigens sp. nov. and Azoarcus communis sp. nov.

“…3H-labeled rRNAs from isolates BH72 and H6a2 were prepared and purified (9) by using [3H]adenine and [ 3H]uracil as precursors; the specific activities of the 23s rRNA preparations were 21 x lo3 and 11 X lo3 cpm wg-' , respectively. Labeled rRNAs from the following reference organisms were available from members of our research group: Alteromonas haloplanktis LMG 2852T (T = type strain) (SO), Xanthomonas cam estris LMG 56gT illum linum LMG 5414T (36), Azorhizobium caulinodans LMG 6465T (14), Aquaspirillum sevens biovar bengal strain LMG 6234T (37), [Pseudomonas J rubrisubalbicans LMG 2286T (18), Hydrogenophaga palleronii LMG 2366T (52), and Oligella urethralis LMG 5304 (45). Hybridizations between filter-fixed DNA and labeled 23s rRNA were carried out as described previously (9).…”

Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis.

“…Since the first reported outbreaks in 1977 in England [7] and Ireland [23], CEM has spread worldwide [15,22] and remains a considerable economical problem among horse breeders in some countries including Europe [25] and Japan. The causative agent of CEM, Taylorella equigenitalis, is a gramnegative, nonmotile, coccoid rod which is classified in the family Pasteurellaceae and whose closest phylogenetic relative is Oligella urethralis [2,4,5,16].…”