Olfactory response of Anastrepha striata (Diptera: Tephritidae) to guava and sweet orange volatiles

Abstract

The behavioral responses of virgin and mated female Anastrepha striata Schiner (Diptera: Tephritidae) to guava (Psidium guajava L.) or sweet orange (Citrus sinensis L.) were evaluated separately using multilure traps in two-choice tests in field cages. The results showed that flies were more attracted to guava and sweet orange volatiles than to control (unbaited trap). The physiological state (virgin or mated) of females did not affect their attraction to the fruit volatiles. Combined analysis of gas chromatography coupled with electroantennography (GC-EAD) of volatile extracts of both fruits showed that 1 and 6 compounds from orange and guava, respectively elicited repeatable antennal responses from mated females. The EAD active compounds in guava volatile extracts were identified by gas chromatography-mass spectrometry (GC-MS) as ethyl butyrate, (Z)-3-hexenol, hexanol, ethyl hexanoate, hexyl acetate, and ethyl octanoate. Linalool was identified as the only antennal active compound in sweet orange extracts. In field cage tests, there were no significant differences between the number of mated flies captured by the traps baited with guava extracts and the number caught by traps baited with the 6-component blend that was formulated according to the relative proportions in the guava extracts. Similar results occurred when synthetic linalool was evaluated against orange extracts. From a practical point of view, the compounds identified in this study could be used for monitoring A. striata populations.

Identification of Blackberry (Rubus fruticosus) Volatiles as Drosophila suzukii Attractants

“…Around the same time, linalool was shown to have a strong insecticidal effect on D. suzukii when used at a high concentration as a fumigant [ 50 ]. Linalool is also recognized by and has shown to be attractive to other Diptera such as Tephritidae and the housefly ( Musca domestica ) [ 51 , 52 , 53 , 54 ].…”

Tools in the Investigation of Volatile Semiochemicals on Insects: From Sampling to Statistical Analysis

“…There are two main classifications of HS sampling [35]: static (SHS), in which the analytes on the vapor phase are accumulated passively on capture material by sorption; and dynamic (DHS), which employs a gas flow to assist with the extraction and concentration of volatile compounds on the adsorbent phase [36]. There are a huge number of published cases using DHS sampling, so the collection of VOCs has been achieved, for instance, from whole intact plants [37] or those with controlled herbivory [38,39], flowers [40,41], fresh fruits [42], insect dregs [37], roots [43], and living insects [44].…”

“…When comparing the proportion of recent publications on VOCs active on insects that reference the use of GC coupled to single-quadrupole mass spectrometer (qMS) against other analyzers, for the latter, the number of citations is lower. Among these, the utilization of ion trap mass spectrometers (itMS) is referenced in studies addressing VOCs related to insect attraction in flowers [69] and whole plants [73], phytopathogenic vectors [115], and parasites [42]. In parallel, the magnetic sector analyzer has been reported for real-time monitoring of insect pheromone release [44] and time-of-flight (TOF) analyzers in the assessment of biosynthesis of VOCs important for pollinator attraction [21], in the characterization of volatile secretions from Graphosoma lineatum [116], or in the profiling of compounds present in Tilia spp.…”

“…One such configuration separates the insect from the reservoirs by using conduits, and the vapors from each reservoir are pumped to the insect placement point. This has been cited in insect attraction assays regarding whole plants [68,165] or fruits [42], among others. Another interesting experiment with a nonconventional configuration is described in Ref.…”

Bioatividade de extratos botânicos para adultos de Anastrepha fraterculus (Wiedemann) e Ceratitis capitata (Wiedemann) (Diptera: Tephritidae)

“…Quando isolados e testados contra insetos, muitos OE demonstraram atividade inseticida, mas também uma série de efeitos subletais, incluindo a interrupção do crescimento, desenvolvimento, reprodução e comportamento de vários insetos (ISMAN, 2006;REGNAULT-ROGER et al, 2012;WAGAN et al, 2016). Diaz-Santiz et al (2016) analisaram respostas antenais por eletroantenograma de voláteis de frutos de laranja-doce e goiaba em fêmeas virgens ou copuladas de A. striata. Os autores descrevem apenas um volátil de laranja: o álcool linalol; e seis de goiaba: ethyl butyrate, (Z)-3-hexenol, hexanol, ethyl hexanoate, hexyl acetate e ethyl octanoate que geraram respostas nas antenas destes insetos.…”