Olfactory receptor 43 reduces hepatic lipid accumulation and adiposity in mice

Wu, Chunyan; Thach, Trung Thanh; Kim, Yeon Ji; Lee, Sung Joon

doi:10.1016/j.bbalip.2019.01.004

Cited by 28 publications

(30 citation statements)

References 62 publications

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“…C2C12 cells were seeded overnight and differentiated for 7 days. Differentiated skeletal myotubes were transfected with 200 pmol of scramble or Olfr544 siRNA duplex (SantaCruz, CA, United States) with Lipofectamine 2000 reagent (Invitrogen, CA, United States) as previously described (Wu et al, 2019). After transfection for 6 h, differentiated skeletal myotubes were transfected again with the same amount of scramble or Olfr544 siRNA.…”

Section: Double-transfection Of Small Interfering Rna (Sirna)mentioning

confidence: 99%

“…Subsequently, Rever Trace RT Master Mix Kit (Toyobo, Osaka, Japan) was used to synthesize the cDNA according to the manufacturer's instructions using the. Quantitative RT-PCR experiments were then conducted to check the gene expression levels with cDNA as previously described (Jia et al, 2013;Kang et al, 2015;Wu et al, 2019). Templates were amplified by using specific sets of primers listed in Supplementary Table S1 with the Thunderbird TM SYBR qPCR Mix reagent (Takara Bio Inc., Japan) and analyzed by the iQ5 Cycler System (Bio-Rad, Hercules, CA, United States).…”

Section: Quantitative Real-time Rt-pcrmentioning

confidence: 99%

“…We previously reported that OR1A1 and its mouse homolog, Olfr43, regulate lipid metabolism in the liver. OR1A1/Olfr43 stimulated by (-)-carvone reduced hepatic steatosis through regulating the PKA-cAMP-response element binding protein (CREB)-hairy and enhancer of split-1 signaling axis (Wu et al, , 2019. The results from microarray analysis showed that Olfr544 is highly expressed in both mouse liver and white adipose tissue, and activation of Olfr544 stimulates fatty acid oxidation in hepatocytes, lipolysis and thermogenesis in white and brown adipose tissues (Wu et al, 2017), respectively.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Azelaic Acid Induces Mitochondrial Biogenesis in Skeletal Muscle by Activation of Olfactory Receptor 544

Thach

Hwang

et al. 2020

Front. Physiol.

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Section: Double-transfection Of Small Interfering Rna (Sirna)mentioning

confidence: 99%

Section: Quantitative Real-time Rt-pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Azelaic Acid Induces Mitochondrial Biogenesis in Skeletal Muscle by Activation of Olfactory Receptor 544

Thach

Hwang

et al. 2020

Front. Physiol.

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“…Recently, several reports have emerged that implicate ORs in steatosis and glucose metabolism. In cultured hepatocytes, murine Olfr43 has been shown to reduce hepatic lipid accumulation and adiposity while its human homolog (OR1A1) has been shown to reduce PPAR-γ expression ( Wu et al, 2019 ). Another study found that stimulation of Olfr43 led to the secretion of glucose-like phosphoprotein-1 (GLP-1) in enteroendocrine cells, which in turn helps regulate blood glucose levels in a type-2 diabetic mouse model ( Kim et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

The Sensing Liver: Localization and Ligands for Hepatic Murine Olfactory and Taste Receptors

et al. 2020

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Sensory receptors, including olfactory receptors (ORs), taste receptors (TRs), and opsins (Opns) have recently been found in a variety of non-sensory tissues where they have distinct physiological functions. As G protein-coupled receptors (GPCRs), these proteins can serve as important chemosensors by sensing and interpreting chemical cues in the environment. We reasoned that the liver, the largest metabolic organ in the body, is primed to take advantage of some of these sensory receptors in order to sense and regulate blood content and metabolism. In this study, we report the expression of novel hepatic sensory receptors – including 7 ORs, 6 bitter TRs, and 1 Opn – identified through a systematic molecular biology screening approach. We further determined that several of these receptors are expressed within hepatocytes, the parenchymal cells of the liver. Finally, we uncovered several agonists of the previously orphaned hepatic ORs. These compounds fall under two classes: methylpyrazines and monoterpenes. In particular, the latter chemicals are plant and fungal-derived compounds with known hepatic protective effects. Collectively, this study sheds light on the chemosensory functions of the liver and unveils potentially important regulators of hepatic homeostasis.

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“…Carvone has been designated as a generally recognized as safe (GRAS) substance for application as a flavoring agent in the food industry. This compound is well known for its antioxidant, antimicrobial, hypotensive, and hypolipidemic effects [20][21][22]. In the present study, we sought to examine whether carvone decreases melanin content by inhibiting the proliferation of melanoma cells, and if so, to explore whether carvone exerts its antiproliferative effect through the cAMP pathway.…”

Section: Introductionmentioning

confidence: 99%

Carvone Decreases Melanin Content by Inhibiting Melanoma Cell Proliferation via the Cyclic Adenosine Monophosphate (cAMP) Pathway

2020

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Melanin, which determines the color of the skin and hair, is initially synthesized to protect the skin from ultraviolet light; however, excessive melanin pigmentation caused by abnormal cell proliferation can result in various melanocytic lesions. Cyclic adenosine monophosphate (cAMP) is known to regulate cell cycle progression and consequently to inhibit the division of abnormally proliferating cells. In this work, we aimed to test whether carvone, a scent compound from plants, inhibits proliferation and subsequently reduces melanin content of melanoma cells and to determine whether its beneficial effects are mediated by the cAMP pathway. We found that carvone decreases melanin content and inhibits melanoma cell proliferation in a concentration-dependent manner. Meanwhile, it inhibited the activation of cell cycle-associated proteins such as cyclin-dependent kinase 1 (CDK1). Of note, the beneficial effects of carvone were abrogated by cAMP inhibition. Our findings indicate potential benefits of carvone for the treatment of melanomas and presumably other hyperpigmentation-related dermatological disorders such as melasmas, lentigines, and excessive freckles.

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Olfactory receptor 43 reduces hepatic lipid accumulation and adiposity in mice

Cited by 28 publications

References 62 publications

Azelaic Acid Induces Mitochondrial Biogenesis in Skeletal Muscle by Activation of Olfactory Receptor 544

Azelaic Acid Induces Mitochondrial Biogenesis in Skeletal Muscle by Activation of Olfactory Receptor 544

The Sensing Liver: Localization and Ligands for Hepatic Murine Olfactory and Taste Receptors

Carvone Decreases Melanin Content by Inhibiting Melanoma Cell Proliferation via the Cyclic Adenosine Monophosphate (cAMP) Pathway

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