Olfactory ensheathing glia: Their contribution to primary olfactory nervous system regeneration and their regenerative potential following transplantation into the injured spinal cord

Franssen, Elske H.P.; Bree, F.M. de; Verhaagen, Joost

doi:10.1016/j.brainresrev.2007.07.013

Cited by 147 publications

(107 citation statements)

References 208 publications

Supporting

Mentioning

104

Contrasting

Unclassified

Order By: Relevance

“…Since olfactory ensheathing cells (OECs) have been reported to enhance axonal regeneration in rat spinal cord when transplanted into lesion sites (Li et al, 1997;Ramon-Cueto et al, 1998), OECs have become a prime candidate for cell-mediated repair following a variety of Central Nervous System (CNS) lesions (Richter and Roskams, 2008) not only in animal model but also in clinical situation (Li et al, 1998;Dobkin et al, 2006;Franssen et al, 2007;Bauchet et al, 2008). More and more literature reviews give us more and more hope that OEC transplantation to be one of the most promising therapeutic strategies (Barnett and Riddell, 2007;Sasaki et al, 2007;Bauchet et al, 2008;Bunge, 2008;Radtke et al, 2008;Richter and Roskams, 2008;Kawaja et al, 2009); OECs have been successfully transplanted in acute (Resnick et al, 2003;Polentes et al, 2004;Collazos-Castro et al, 2005;Lopez-Vales et al, 2006;Andrews and Stelzner, 2007;Sasaki et al, 2007) and chronic (Andrews and Stelzner, 2004;Lopez-Vales et al, 2007) models of rodent spinal cord injury.…”

mentioning

confidence: 99%

The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration

Liu²,

Wang³

et al. 2010

The Anatomical Record

View full text Add to dashboard Cite

Clinical studies have expanded the therapeutic olfactory ensheathing cells (OECs) transplantation to different human Central Nervous System (CNS) diseases. In fact, the OEC transplantation in clinic is a mixture of olfactory bulb cells; they even have not demonstrated that they have such a subpopulation yet. However, as a source of OECs transplantation, the development and identification of human fetal OECs are still need more understanding, because some surgery try to restoration CNS injury with a more purity of OEC cultures generated by a number of different procedures. In this article, twelve human fetal olfactory bulb (OB) samples were obtained from six fetuses in 20 weeks of gestation, it was studied by immunofluorescence on histological sections and cultured cells with multiple antibodies under confocal microscopy. The P75NTR positive OB-OECs (olfactory ensheathing cell from the olfactory bulb) were present in both outer olfactory nerve layers and glomerular layer. The percentage of OB cells in culture, about 22.31 was P75NTR positive, 45.77 was S100b, and 31.92 was GFAP. P75NTR and GFAP were coexpressed with S100b, respectively; however, P75NTR was not coexpressed with GFAP in human fetal OECs. It is suggested that the localization and development of human OECs in OB are different to those in rodent, and the P75NTR immunohistological staining is still necessary to identify and characterize human fetal OECs in culture before transplantation. Anat Rec, 293:359-369, 2010. V V C 2009 Wiley-Liss, Inc.

show abstract

mentioning

confidence: 99%

The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration

Liu²,

Wang³

et al. 2010

The Anatomical Record

View full text Add to dashboard Cite

show abstract

“…Following injury, the limited inherent capacity for repair possessed by the central nervous system (CNS) means that therapeutic measures must be undertaken to compensate for this deficiency (Pearse and Barakat, 2006;Pearse and Bunge, 2006). The implantation of exogenous cells, derived from a variety of sources, has been demonstrated to be an effective approach for overcoming many of the barriers to successful spinal-cord repair, as well as promoting improved functional outcome (Franssen et al, 2007;Louro and Pearse, 2008;Nandoe Tewarie et al, 2007;Pearse and Barakat, 2006) The implantation of Schwann cells (SCs), the myelinating glia of the peripheral nervous system (PNS) important for facilitating regeneration after nerve injury, has been shown to provide neuroprotection Schaal et al, 2007;Takami et al, 2002), support axonal regrowth and remyelination, Pearse et al, 2004Pearse et al, , 2007, as well as improve functional outcome in experimental models of both acute and chronic spinal cord injury (SCI) (Barakat et al, 2005). Their reparative efficacy, however, may be limited by their survival rate post implantation; approximately 20% of grafted SCs survive within the subacutely (Hill et al, 2007;Pearse et al, 2007) or chronically (Barakat et al, 2005) contused spinal cord.…”

mentioning

confidence: 99%

Suspension Matrices for Improved Schwann-Cell Survival after Implantation into the Injured Rat Spinal Cord

Patel

Joseph

Patel

et al. 2010

Journal of Neurotrauma

View full text Add to dashboard Cite

Trauma to the spinal cord produces endogenously irreversible tissue and functional loss, requiring the application of therapeutic approaches to achieve meaningful restoration. Cellular strategies, in particular Schwanncell implantation, have shown promise in overcoming many of the obstacles facing successful repair of the injured spinal cord. Here, we show that the implantation of Schwann cells as cell suspensions with in-situ gelling laminin:collagen matrices after spinal-cord contusion significantly enhances long-term cell survival but not proliferation, as well as improves graft vascularization and the degree of axonal in-growth over the standard implantation vehicle, minimal media. The use of a matrix to suspend cells prior to implantation should be an important consideration for achieving improved survival and effectiveness of cellular therapies for future clinical application.

show abstract

“…This ubiquitous β-herpesvirus exists in two variants that share 95% sequence homology, HHV-6A and HHV-6B (12). HHV-6B is the etiologic agent for roseola in children and is usually contracted by 2 years of age, the time of exposure and symptoms associated with HHV-6A remains unknown (13).…”

mentioning

confidence: 99%

Human herpesvirus-6 entry into the central nervous system through the olfactory pathway

Harberts

Yao

Wohler

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

135

View full text Add to dashboard Cite

Viruses have been implicated in the development of neurodegenerative diseases, such as Alzheimer's, Parkinson’s, and multiple sclerosis. Human herpesvirus-6 (HHV-6) is a neurotropic virus that has been associated with a wide variety of neurologic disorders, including encephalitis, mesial temporal lobe epilepsy, and multiple sclerosis. Currently, the route of HHV-6 entry into the CNS is unknown. Using autopsy specimens, we found that the frequency of HHV-6 DNA in the olfactory bulb/tract region was among the highest in the brain regions examined. Given this finding, we investigated whether HHV-6 may infect the CNS via the olfactory pathway. HHV-6 DNA was detected in a total of 52 of 126 (41.3%) nasal mucous samples, showing the nasal cavity is a reservoir for HHV-6. Furthermore, specialized olfactory-ensheathing glial cells located in the nasal cavity were demonstrated to support HHV-6 replication in vitro. Collectively, these results support HHV-6 utilization of the olfactory pathway as a route of entry into the CNS.

show abstract

Olfactory ensheathing glia: Their contribution to primary olfactory nervous system regeneration and their regenerative potential following transplantation into the injured spinal cord

Cited by 147 publications

References 208 publications

The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration

The Immunohistochemical Characterization of Human Fetal Olfactory Bulb and Olfactory Ensheathing Cells in Culture as a Source for Clinical CNS Restoration

Suspension Matrices for Improved Schwann-Cell Survival after Implantation into the Injured Rat Spinal Cord

Human herpesvirus-6 entry into the central nervous system through the olfactory pathway

Contact Info

Product

Resources

About