Olfactory ensheathing cells promote proliferation and inhibit neuronal differentiation of neural progenitor cells through activation of Notch signaling

Zhang, J.; Wang, B.; Xiao, Zhifeng; Zhao, Yannan; Chen, B.; Han, Jin Soo; Gao, Yuan; Ding, Wenbing; Zhang, H.; Dai, Jianwu

doi:10.1016/j.neuroscience.2008.02.067

Cited by 23 publications

(18 citation statements)

References 52 publications

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“…Several groups have previously speculated on the potential synergistic effects of their combination 57,58 but none have shown direct or indirect positive influence on differentiation. The response of NSCs to OEC conditioned media in this study disagrees with the findings of a recent in vitro study suggesting that OECs have an inhibitory effect on neuronal differentiation and instead promote NSC proliferation 59 . Zhang et al 59 also found that the majority of NSCs in the NSC media control group differentiated into GFAP positive cells at the 8 day time point, in contrast to the limited differentiation of any kind observed in the NSC media group of our study.…”

Section: Discussioncontrasting

confidence: 99%

Olfactory Ensheathing Cells Promote Differentiation of Neural Stem Cells and Robust Neurite Extension

Sethi

Redmond

et al. 2014

Stem Cell Rev and Rep

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Aims The goal of this study was to gain insight into the signaling between olfactory ensheathing cells (OECs) and neural stem cells (NSCs). We sought to understand the impact of OECs on NSC differentiation and neurite extension and to begin to elucidate the factors involved in these interactions to provide new targets for therapeutic interventions. Materials and Methods We utilized lines of OECs that have been extremely well characterized in vitro and in vivo along with well studied NSCs in gels to determine the impact of the coculture in three dimensions. To further elucidate the signaling, we used conditioned media from the OECs as well as fractioned components on NSCs to determine the molecular weight range of the soluble factors that was most responsible for the NSC behavior. Results We found that the coculture of NSCs and OECs led to robust NSC differentiation and extremely long neural processes not usually seen with NSCs in three dimensional gels in vitro. Through culture of NSCs with fractioned OEC media, we determined that molecules larger than 30 kDa have the greatest impact on the NSC behavior. Conclusions Overall, our findings suggest that cocultures of NSCs and OECs may be a novel combination therapy for neural injuries including spinal cord injury (SCI). Furthermore, we have identified a class of molecules which plays a substantial role in the behavior that provides new targets for investigating pharmacological therapies.

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Section: Discussioncontrasting

confidence: 99%

Olfactory Ensheathing Cells Promote Differentiation of Neural Stem Cells and Robust Neurite Extension

Sethi

Redmond

et al. 2014

Stem Cell Rev and Rep

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“…These findings suggest that Jag1/Notch signaling from EPCs does not enhance migration of reactive astrocytes but that it enhances astrogliosis. On the other hand, previous studies showed that vascular endothelial cells or olfactory ensheathing cells promoted proliferation of neural stem/progenitor cells through activation of Notch signaling (Shen et al, 2004;Zhang et al, 2008). We therefore speculate that transplanted EPCs enhanced proliferation of nestin-positive neural progenitor cells via Jag1-Notch dependent mechanisms in the present study.…”

Section: Jagged1-notch Signaling Promotes Astrogliosis Following Scisupporting

confidence: 42%

Endothelial Progenitor Cells Promote Astrogliosis following Spinal Cord Injury through Jagged1-Dependent Notch Signaling

Kamei

Kwon

Ishikawa

et al. 2012

Journal of Neurotrauma

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Interactions between endothelial and neural stem cells are believed to play a critical role in the kinetics of neural stem cells in the central nervous system. Here we demonstrate that endothelial progenitor cells promote the repair of injured spinal cord through the induction of Notch-dependent astrogliosis and vascular regulation. The transplantation of Jagged1(+/+) endothelial progenitor cells, but not Jagged1(-/-) endothelial progenitor cells, increased the number of reactive astrocytes during the acute phase, and improved functional recovery following spinal cord injury. Expression of the Notch effector Hes5 was upregulated in the injured spinal cord after Jagged1(+/+) endothelial progenitor cell transplantation. Furthermore, we found that the Notch ligand Delta-like-1 was highly expressed in Jagged1(-/-) endothelial progenitor cells. Transplantation of Delta-like-1, as well as Jagged1-overexpressing 3T3 cells, revealed that only Jagged1-overexpressing 3T3 stromal cells enhanced astrogliosis following spinal cord injury. In addition, Jagged1(+/+) endothelial progenitor cells exhibited not only dramatic pro-angiogenic effects, but also morphologically abnormal vessel stabilization, compared with Jagged1(-/-)endothelial progenitor cells in injured spinal cord. Thus, transplanted endothelial progenitor cells promote astrogliosis, vascular regulation, and spinal cord regeneration through activation of Jagged1-Notch signaling.

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“…Previous report showed that condition medium from OECs could promote proliferation of neural stem cells from cortex (Zhang et al, 2008). To evaluate the role of proliferation in the migration assay, we performed BrdU assay.…”

Section: Oec-cm Promotes Progenitors Migration From Rms Neurospheresmentioning

confidence: 99%

Olfactory ensheathing cells: Attractant of neural progenitor migration to olfactory bulb

Zhu

Cao

et al. 2010

Glia

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Olfactory ensheathing cells (OECs) are the glial cells that derive from the olfactory placode, envelop olfactory axons in the course of migration from the olfactory epithelium to the olfactory bulb and reside primarily in the olfactory nerve layer. OECs transplantation as a promising experimental therapy for axonal injuries has been intensively studied; however, little is known about their roles in olfactory bulb development. In this study, we examined the effects of OECs on the migration of neural progenitors in rostral migratory stream (RMS). Initially, the neurosphere migration assay showed that OEC-conditioned medium promoted progenitors to migrate from RMS neurospheres in a concentration dependent manner. Moreover, co-culturing OECs nearby the RMS explants led to asymmetric migration of explants in different developing stages. However, OECs could influence the migration in a distance not further than 1.5 mm. Finally, slice assay that mimic the circumstance in vivo revealed that OECs had a chemoattractive activity on RMS neural progenitors. Together, these results demonstrate that OECs attract neural progenitors in RMS through the release of diffusible factors and it is likely that OECs mainly influence radial migration in the olfactory bulb but not tangential migration of the RMS invivo during development. This suggests a previously unknown function for OECs in olfactory development and a novel mechanism underlying the targeting of RMS cells.

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Olfactory ensheathing cells promote proliferation and inhibit neuronal differentiation of neural progenitor cells through activation of Notch signaling

Cited by 23 publications

References 52 publications

Olfactory Ensheathing Cells Promote Differentiation of Neural Stem Cells and Robust Neurite Extension

Olfactory Ensheathing Cells Promote Differentiation of Neural Stem Cells and Robust Neurite Extension

Endothelial Progenitor Cells Promote Astrogliosis following Spinal Cord Injury through Jagged1-Dependent Notch Signaling

Olfactory ensheathing cells: Attractant of neural progenitor migration to olfactory bulb

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