Olfactory ensheathing cells (OECs) and the treatment of CNS injury: advantages and possible caveats

Barnett, Susan C.; Riddell, John S.

doi:10.1111/j.1469-7580.2004.00257.x

Cited by 133 publications

(88 citation statements)

References 94 publications

(188 reference statements)

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“…Cultured OEC's by our team demonstrated two different phenotypes after attaching to flask surface which was in accordance with other researchers who also reported two subpopulations of OEC's (31, 36). Barnet and Riddle exhibited two subtypes of OEC's that were purified from the olfactory bulb of 7-day old SpragueDawly rats' pups after 12 days that was in agreement with our findings (37). Ensheathing cell morphology is categorized as flat or process-bearing.…”

Section: Discussionsupporting

confidence: 81%

Purity determining of cultured OECs from olfactory mucosa of rats\' pups

Taghi

Bakhtiari

Farhid

et al. 2016

JBRMS

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Introduction: Cell transplantation is one of the main strategies for spinal cord injury repair. As OECs of the olfactory mucosa can be obtained by simple biopsy in all individuals without affecting their smell sensation, OECs considered as a promising candidate for autologous transplantation in the nervous system injury, especially for spinal cord repair. Thereby in the current study OECs were cultured from olfactory mucosa of 7 days old rats' pups and their purity was examined by flow-cytometry after simultaneous double staining for p75 and GFAP markers. Materials and methods: 7 days old Wistar rats' pups were deeply anesthetized by ketamine / xylazine (60/6mg/Kg). Then the nasal cavity was opened sagittally and the olfactory mucosa was separated from posterior part of nasal septum and at last OECs were obtained from lamina properia of olfactory mucosa and were cultured. The cultured cells were simultaneously immunolabeled for p75 and GFAP markers and finally purity of cultured cells assessed by flow-cytometry. Results: cultured OECs demonstrated two different morphologies, a spindle shape Schwannlike and an astrocyte-like OECs with flat sheet-like morphology. In addition, simultaneous immunolabeling for p75 and GFAP markers of OECs exhibited OECs were positive for both markers at the same time. The flow-cytometry results displayed that 87.9±2.4% of cells were p75/ GFAP double positive cells,1.05±0.4 only p75 positive and 5.8±1.5% were single positive for GFAP. Conclusion: Purity of cultured OECs in our study is probably more than 87.9% by flowowing to p75+/S100+ and GFAP+/S100+ olfactory ensheathing cells were not counted. Thus the culture procedure of this study seems to be a good protocol for OECs purifying and cell therapy in CNS damages.

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Section: Discussionsupporting

confidence: 81%

Purity determining of cultured OECs from olfactory mucosa of rats\' pups

Taghi

Bakhtiari

Farhid

et al. 2016

JBRMS

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“…Although we did not observe any OECs in the graft core or in the infarct border in these animals, the finding of a vascular network in However, in the absence of an unequivocal marker for OECs [40,41], further immunostaining (i.e. in addition to anti-GFP antibody) for the purpose of identifying more surviving/migrating OECs was not relevant.…”

Section: Discussioncontrasting

confidence: 57%

Effects of Neural Stem Cell and Olfactory Ensheathing Cell Co-transplants on Tissue Remodelling After Transient Focal Cerebral Ischemia in the Adult Rat

et al. 2017

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Effects of neural stem cell and olfactory ensheathing cell cotransplants on tissue remodelling after transient focal cerebral ischemia in the adult rat. Neurochemical Research, 42(6), pp. 1599-1609.There may be differences between this version and the published version. You are advised to consult the publisher's version if you wish to cite from it.http://eprints.gla.ac.uk/136264/ with the host-tissue post-transplantation is poor. In this study, we address this challenge by testing whether co-grafting of NSCs with olfactory ensheathing cells (OECs), a special type of glia with proven neuroprotective, immunomodulatory, and angiogenic effects, can promote graft survival and host tissue remodelling. Transient focal cerebral ischemia was induced in adult rats by a sixty-minute middle cerebral artery occlusion (MCAo) followed by reperfusion. Ischemic lesions were verified by neurological testing and magnetic resonance imaging (MRI). Transplantation into the globus pallidus of NSCs alone or in combination with OECs was performed at two weeks post-MCAo, followed by histological analyses at three weeks post-transplantation. We found evidence of extensive vascular remodelling in the ischemic core as well as evidence of NSC motility away from the graft and into the infarct border in severely lesioned animals co-grafted with OECs. These findings support a possible role of OECs as part of an in situ tissue engineering paradigm for transplant mediated repair of ischemic brain lesions.

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“…Another benefit of these cells is that in some cases they have been suggested to restore a degree of functions lost due to the CNS lesions. However, there is very little immunological data to support this conclusion, which was derived mainly from behavioural tests (Barnett and Riddell 2004). It is thought that the most useful way to utilise this type of cells may be to use them in parallel with other synergistic treatments.…”

Section: Olfactory Ensheathing Cellmentioning

confidence: 98%