Olfactory ensheathing cells from the nasal mucosa and olfactory bulb have distinct membrane properties

Smith, Katie E.; Whitcroft, Katherine L.; Law, S.S.; Andrews, Peter; Choi, David; Jagger, Daniel J.

doi:10.1002/jnr.24566

Cited by 7 publications

(3 citation statements)

References 50 publications

(101 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As has been described for other glial cells (Djukic et al, 2007;Olsen et al, 2006;Seifert et al, 2009;Smith et al, 2020), blockade of the weak inwardly rectifying current in spiral ganglion SGCs by Ba 2+ or desipramine led to membrane depolarization, suggesting that this current is important for setting their resting membrane potential. Within astrocytes, the hyperpolarizing effect of Kir4.1 on the resting membrane potential is proposed to maximize synaptic glutamate clearance during neuronal activity (Olsen & Sontheimer, 2008).…”

Section: Physiological Roles For Weak Inwardly Rectifying Currents supporting

confidence: 67%

Divergent membrane properties of mouse cochlear glial cells around hearing onset

Smith

Murphy

Jagger

2020

J of Neuroscience Research

Self Cite

View full text Add to dashboard Cite

Spiral ganglion neurons (SGNs) are the primary afferent neurons of the auditory system, and together with their attendant glia, form the auditory nerve. Within the cochlea, satellite glial cells (SGCs) encapsulate the cell body of SGNs, whereas Schwann cells (SCs) wrap their peripherally-and centrally-directed neurites. Despite their likely importance in auditory nerve function and homeostasis, the physiological properties of auditory glial cells have evaded description. Here, we characterized the voltage-activated membrane currents of glial cells from the mouse cochlea. We identified a prominent weak inwardly rectifying current in SGCs within cochlear slice preparations (postnatal day P5-P6), which was also present in presumptive SGCs within dissociated cultures prepared from the cochleae of hearing mice (P14-P15). Pharmacological block by Ba 2+ and desipramine suggested that channels belonging to the Kir4 family mediated the weak inwardly rectifying current, and post hoc immunofluorescence implicated the involvement of Kir4.1 subunits. Additional electrophysiological profiles were identified for glial cells within dissociated cultures, suggesting that glial subtypes may have specific membrane properties to support distinct physiological roles. Immunofluorescence using fixed cochlear sections revealed that although Kir4.1 is restricted to SGCs after the onset of hearing, these channels are more widely distributed within the glial population earlier in postnatal development (i.e., within both SGCs and SCs). The decrease in Kir4.1 immunofluorescence during SC maturation was coincident with a reduction of Sox2 expression and advancing neurite myelination. The data suggest a diversification of glial properties occurs in preparation for sound-driven activity in the auditory nerve.

show abstract

Section: Physiological Roles For Weak Inwardly Rectifying Currents supporting

confidence: 67%

Divergent membrane properties of mouse cochlear glial cells around hearing onset

Smith

Murphy

Jagger

2020

J of Neuroscience Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…As a consequence of increased media coverage, the number of patients coming to otolaryngology clinics is also expected to be higher than normal. In addition to current available therapies for OD, 32 there is a need to embrace new therapies which explore the regeneration of damaged neurons 34,35 …”

Section: Discussionmentioning

confidence: 99%

Olfactory and taste dysfunction among mild‐to‐moderate symptomatic COVID‐19 positive health care workers: An international survey

Andrews

Pendolino

Ottaviano

et al. 2020

Laryngoscope Investig Oto

Self Cite

View full text Add to dashboard Cite

Objectives To determine the prevalence of olfactory and taste dysfunction (OD; TD) among COVID‐19 positive health care workers (HCWs), their associated risk factors and prognosis. Methods Between May and June 2020, a longitudinal multicenter study was conducted on symptomatic COVID‐19 PCR confirmed HCWs (COVID‐19 positive) in London and Padua. Results Hundred and fourteen COVID‐19 positive HCWs were surveyed with a response rate of 70.6% over a median follow‐up period of 52 days. UK prevalence of OD and TD was 73.1% and 69.2%, respectively. There was a male to female ratio of 1:3 with 81.6% being white, 43.7% being nurses/health care assistants (HCAs), and 39.3% being doctors. In addition, 53.2% of them worked on COVID‐19 wards. Complete recovery was reported in 31.8% for OD and 47.1% for TD with a 52 days follow‐up. The job role of doctors and nurses negatively influenced smell ( P = .04 and P = .02) and taste recovery ( P = .02 and P = .01). Ethnicity (being white) showed to positively influence only taste recovery ( P = .04). Sex (being female) negatively influenced OD and TD recovery only in Paduan HCWs ( P = .02 and P = .011, respectively). Working on a COVID‐19 ward did not influence prognosis. Conclusions The prevalence of OD and TD was considerably higher in HCWs. The prognosis for OD and TD recovery was worse for nurses/HCAs and doctors but working on a COVID‐19 ward did not influence prognosis. Sixty‐eight percent of surveyed HCWs at 52 days continued to experience OD or TD requiring additional future medical management capacity. Level of Evidence 4.

show abstract

“…Indeed, as described above, OECs ensheathe PONs and can be found in the PNS, in the OE, or in the CNS, in the ONL of the OB. Several studies have reported that, according to their origin, OM or OB, OECs express distinct properties in vitro [18,19]. Thereafter, based on microarray analyses, we have demonstrated that OECs from OM and OECs from OB, respectively OM-OECs and OB-OECs, display distinct gene expression patterns.…”

Section: Origin and Cellular Heterogeneity Of The Oecsmentioning

confidence: 73%

Transplantation of Olfactory Ensheathing Cells: Properties and Therapeutic Effects after Transplantation into the Lesioned Nervous System

Delarue

Guérout

2022

Neuroglia

View full text Add to dashboard Cite

The primary olfactory system (POS) is in permanent renewal, especially the primary olfactory neurons (PON) are renewed with a turnover of around four weeks, even in adulthood. The re-growth of these axons is helped by a specific population of glial cells: the olfactory ensheathing cells (OECs). In the POS, OECs constitute an “open-channel” in which the axons of PON cause regrowth from peripheral nervous system (PNS) to central nervous system (CNS). The remarkable role played by OECs into the POS has led scientists to investigate their properties and potential beneficial effects after transplantation in different lesion models of the CNS and PNS. In this review, we will resume and discuss more than thirty years of research regarding OEC studies. Indeed, after discussing the embryonic origins of OECs, we will describe the in vitro and in vivo properties exert at physiological state by these cells. Thereafter, we will present and talk over the effects of the transplantation of OECs after spinal cord injury, peripheral injury and other CNS injury models such as demyelinating diseases or traumatic brain injury. Finally, the mechanisms exerted by OECs in these different CNS and PNS lesion paradigms will be stated and we will conclude by presenting the innovations and future directions which can be considered to improve OECs properties and allow us to envisage their use in the near future in clinical applications.

show abstract

Olfactory ensheathing cells from the nasal mucosa and olfactory bulb have distinct membrane properties

Cited by 7 publications

References 50 publications

Divergent membrane properties of mouse cochlear glial cells around hearing onset

Divergent membrane properties of mouse cochlear glial cells around hearing onset

Olfactory and taste dysfunction among mild‐to‐moderate symptomatic COVID‐19 positive health care workers: An international survey

Transplantation of Olfactory Ensheathing Cells: Properties and Therapeutic Effects after Transplantation into the Lesioned Nervous System

Contact Info

Product

Resources

About