Olfactory Control of Blood Progenitor Maintenance

Shim, Jiwon; Mukherjee, Tina; Mondal, Bama Charan; Liu, Ting; Young, Gloria Chin; Wijewarnasuriya, Dinali; Banerjee, Utpal

doi:10.1016/j.cell.2013.10.032

Cited by 115 publications

(184 citation statements)

References 68 publications

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“…Thus, ARF1 negatively regulates differentiation and we found that this is due to its effect on the hematopoietic stem cell niche (PSC). Recent studies showed that lymph gland hematopoiesis is regulated by autonomous as well as systemic factors (26,27). Our analysis shows an essential resident role for ARF1 in the niche as well as for communication from the nonlymph-gland hemocytes.…”

Section: Discussionmentioning

confidence: 53%

ARF1–GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis

Khadilkar

Rodrigues

Mote

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance Ras small GTPases including ARFs act as molecular switches to modulate signaling pathways involved in hematopoiesis. Decreased guanine nucleotide exchange factor activity or increased GTPase-activating protein activation are associated with many leukemias, myelodysplastic syndromes, and myeloproliferative disorders. Drosophila is a good model to address questions related to aberrant hematopoiesis. Using Drosophila genetics and gene expression analysis we show tissue-specific function of the ubiquitously expressed endocytic protein, Drosophila ARF1 by interaction of ARF1–GTP with a blood-cell–expressed endocytic protein Asrij. The ARF1–Asrij axis brings about endosomal regulation of multiple signaling pathways in hematopoiesis.

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Section: Discussionmentioning

confidence: 53%

ARF1–GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis

Khadilkar

Rodrigues

Mote

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Of note, however, the PSC is not orchestrating the LG response to all systemic inputs. For instance, food deprivation or odors have been found to directly impinge on prohemocyte fate (17,31). All together, these findings underscore the regulatory role of the PSC, which acts as a signaling hub to fine-tune hematopoiesis but whose presence is not essential for LG homeostasis under laboratory breeding conditions.…”

Section: Discussionmentioning

confidence: 93%

The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche

Benmimoun

Polesello

Haenlin

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

149

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The maintenance of stem or progenitor cell fate relies on intrinsic factors as well as local cues from the cellular microenvironment and systemic signaling. In the lymph gland, an hematopoietic organ in Drosophila larva, a group of cells called the Posterior Signaling Centre (PSC), whose specification depends on the EBF transcription factor Collier (Col) and the HOX factor Antennapedia (Antp), has been proposed to form a niche required to maintain the pool of hematopoietic progenitors (prohemocytes). In contrast with this model, we show here that genetic ablation of the PSC does not cause an increase in blood cell differentiation or a loss of blood cell progenitors. Furthermore, although both col and Antp mutant larvae are devoid of PSC, the massive prohemocyte differentiation observed in col mutant is not phenocopied in Antp mutant. Interestingly, beside its expression in the PSC, Col is also expressed at low levels in prohemocytes and we show that this expression persists in PSC-ablated and Antp mutant larvae. Moreover, targeted knockdown and rescue experiments indicate that Col expression is required in the prohemocytes to prevent their differentiation. Together, our findings show that the PSC is dispensable for blood cell progenitor maintenance and reveal the key role of the conserved transcription factor Col as an intrinsic regulator of hematopoietic progenitor fate.hematopoiesis | Drosophila | stem cell niche | EBF

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“…17 Briefly, the immunoassay was performed after extraction and derivatization of GABA from experimental samples. The antigen was bound to the solid phase of the microtiter plate.…”

Section: Detection Of Gaba By Elisamentioning

confidence: 99%

γ-Aminobutyric Acid Is Synthesized and Released by the Endothelium

Sen

Roy

Bandyopadhyay

et al. 2016

Circulation Research

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Rationale: Gamma aminobutyric acid (GABA), a neurotransmitter of the central nervous system, is found in the systemic circulation of humans at a concentration between 0.5 and 3 μmol/L. However, the potential source of circulating GABA and its significance on the vascular system remains unknown. We hypothesized that endothelial cells (ECs) may synthesize and release GABA to modulate some functions in the EC and after its release into the circulation.Objective: To assess whether GABA is synthesized and released by the EC and its potential functions. Methods and Results: Utilizing the human umbilical vein ECs and aortic ECs, we demonstrated for the first time that ECs synthesize and release GABA from [1-14 C]glutamate. Localization of GABA and the presence of the GABA-synthesizing enzyme, glutamic acid decarboxylase in EC were confirmed by immunostaining and immunoblot analysis, respectively. The presence of GABA was further confirmed by immunohistochemistry in the EC lining the human coronary vessel. EC-derived GABA regulated the key mechanisms of ATP synthesis, fatty acid, and pyruvate oxidation in EC. GABA protected EC by inhibiting the reactive oxygen species generation and prevented monocyte adhesion by attenuating vascular cell adhesion molecule -1 and monocyte chemoattractant protein-1 expressions. GABA had no relaxing effect on rat aortic rings. GABA exhibited a dose-dependent fall in blood pressure. However, the fall in BP was abolished after pretreatment with pentolinium. from Perkin Elmer, MA, catalog NEC290E050UC, NEC255050UC, and NEC559010UC. All other chemicals unless specified were obtained from Sigma. Conclusions: Cell CultureHuman umbilical vein endothelial cells (HUVECs) were freshly isolated from umbilical cords from different donors after normal delivery (with approval from the Institutional Review Board of University of California at Los Angeles, LA, CA). Primary cultures of HUVEC were prepared as described 14 and used at passages 1 or 2. Primary cultures of human aortic endothelial cells (HAEC) at passage 2 or 3 were a generous gift from Dr M. Navab in the Department of Medicine, University of California, Los Angeles, 90095-1732, USA. Both HAEC and HUVEC were cultured in endothelial cell basal medium-2 (Lonza), and the medium was supplemented with Endothelial Growth Medium-2 Bullet kit (CC3162; Lonza) as per the manufacturer's instructions as described previously. 15PC12 cells were a generous gift from Dr E. Schweitzer, Semel Institute of Neuroscience and Human Behavior, University of California, Los Angeles. PC12 cells were cultured in DMEM supplemented with 5% FCS and 5% Horse serum. Human skin fibroblast (HSF) cells were a gift from Dr H. Coller, Molecular Cell and Developmental Biology, University of California, Los Angeles. HSF and THP1 were cultured in DMEM supplemented with 10% FBS. All cells were cultured until 70% confluent before downstream manipulations. Cell culture treatments were done under sterile conditions, for 24 hours unless specified. Glutamic acid decarboxylase (GAD) doubl...

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Olfactory Control of Blood Progenitor Maintenance

Cited by 115 publications

References 68 publications

ARF1–GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis

ARF1–GTP regulates Asrij to provide endocytic control of Drosophila blood cell homeostasis

The EBF transcription factor Collier directly promotes Drosophila blood cell progenitor maintenance independently of the niche

γ-Aminobutyric Acid Is Synthesized and Released by the Endothelium

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