Olfactory Conditioning Impairment Following Posttraining NMDA Receptor Blockade in Neonatal Rats

Weldon, Douglas A.; Fedorcik, Gregory G.; LoRusso, Carmela M.; Tiburzi, Michael J.; Lenoci, Jennifer M.

doi:10.1006/nlme.1996.3744

Cited by 16 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first experiment was conducted using MK801, a non-competitive antagonist of the N-methyl D-aspartate glutamate receptors (NMDAR). This drug has been widely used as anamnesic agent in several memory tasks in different species (Morris et al 1986;Tan et al 1989;Miserendino et al 1990;Lin and Glanzman 1994;Rickard et al 1994;Weldon et al 1997;Gutiérrez et al 1999;Roberts and Glanzman 2003;Si et al 2004;Xia et al 2005;Wu et al 2007;Kano et al 2008;Müssig et al 2010;Rosenegger and Lukowiak 2010;Amano and Maruyama 2011). Moreover, MK801 caused amnesic effects in consolidation (Troncoso and Maldonado 2002), reconsolidation and extinction (Pérez-Cuesta and Maldonado 2009) in Chasmagnathus, consistent with the presence of NMDAR in the crab CNS (Hepp et al 2013).…”

Section: Inhibition Of the Ups Blocks The Action Of Mk801 And Bicuculmentioning

confidence: 78%

Protein degradation by ubiquitin–proteasome system in formation and labilization of contextual conditioning memory

et al. 2014

View full text Add to dashboard Cite

The ubiquitin -proteasome system (UPS) of protein degradation has been evaluated in different forms of neural plasticity and memory. The role of UPS in such processes is controversial. Several results support the idea that the activation of this system in memory consolidation is necessary to overcome negative constrains for plasticity. In this case, the inhibition of the UPS during consolidation impairs memory. Similar results were reported for memory reconsolidation. However, in other cases, the inhibition of UPS had no effect on memory consolidation and reconsolidation but impedes the amnesic action of protein synthesis inhibition after retrieval. The last finding suggests a specific action of the UPS inhibitor on memory labilization. However, another interpretation is possible in terms of the synthesis/degradation balance of positive and negative elements in neural plasticity, as was found in the case of long-term potentiation. To evaluate these alternative interpretations, other reconsolidation-interfering drugs than translation inhibitors should be tested. Here we analyzed initially the UPS inhibitor effect in contextual conditioning in crabs. We found that UPS inhibition during consolidation impaired long-term memory. In contrast, UPS inhibition did not affect memory reconsolidation after contextual retrieval but, in fact, impeded memory labilization, blocking the action of drugs that does not affect directly the protein synthesis. To extend these finding to vertebrates, we performed similar experiments in contextual fear memory in mice. We found that the UPS inhibitor in hippocampus affected memory consolidation and blocked memory labilization after retrieval. These findings exclude alternative interpretations to the requirement of UPS in memory labilization and give evidence of this mechanism in both vertebrates and invertebrates.

show abstract

Section: Inhibition Of the Ups Blocks The Action Of Mk801 And Bicuculmentioning

confidence: 78%

Protein degradation by ubiquitin–proteasome system in formation and labilization of contextual conditioning memory

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Systemic injections of MK-801 have impaired olfactory-water reward discrimination learning and its reversal (Griesbach et al 1998), and olfactory-tactile preference learning in neonatal rats (Weldon et al, 1997). In addition, central administration of the competitive NR antagonist APV has identified critical sites for olfactory associations in different paradigms.…”

Section: Discussionmentioning

confidence: 99%

“…The rats in the first group (MK-801 group, n = 8) received a MK-801 injection (Sigma, St. Louis, MO, USA; 100 μg in 1ml/kg ip) while rats in the second group (vehicle group, n = 8) received a vehicle injection (0.15 M NaCl, 1 ml/kg ip). The dose of MK-801 was selected based on efficacy as an NR antagonist in other learning paradigms, without inducing noticeable motoric side effects (Griesbach et al, 1998;Stuchlik et al, 2004;Weldon et al, 1997). Thirty minutes after the injections, rats were given 2-h access to either the CS+/F or CS−/S solution in a one-bottle conditioning session.…”

Section: Experiments 1: Mk-801 and Cftp Acquisitionmentioning

confidence: 99%

NMDA receptor in conditioned flavor-taste preference learning: Blockade by MK-801 and enhancement by D-cycloserine

Golden

Houpt

2007

Pharmacology Biochemistry and Behavior

View full text Add to dashboard Cite

Conditioned flavor-taste preference (CFTP) is a robust form of learning in which animals acquire a preference for a flavor (e.g. Kool-Aid) previously mixed with a highly preferred tastant (e.g. fructose) over a flavor previously mixed with a less preferred tastant (e.g. saccharin). Here, the role of the Nmethyl-D-aspartate (NMDA) glutamate-glycine receptor (NR) was probed using systemic MK-801, a non-competitive antagonist, and D-cycloserine (DCS), a glycine agonist. Rats were injected with MK-801 (100 μg/kg) or vehicle 30 min prior to a daily 2-h conditioning session with 1-bottle access to a Kool-Aid flavor (grape or cherry) mixed with either 8% fructose (CS+/F) or 0.2% saccharin (CS −/S). CFTP expression was measured in 2-bottle preference tests between the Kool-Aid flavors mixed with 0.2% saccharin (CS+/S vs. CS−/S). While vehicle-treated rats acquired a preference for CS+/ S over CS−/S, MK-801 prior to conditioning completely blocked CFTP learning. The effect of MK-801 was specific to CFTP acquisition, because follow-up experiments demonstrated that MK-801 did not induce a conditioned taste aversion, cause state-dependent learning, or affect CFTP expression. In a second approach, rats were injected with DCS (15 mg/kg) 60 min prior to daily conditioning. In contrast to MK-801, adminstration of DCS prior to conditioning enhanced CFTP learning (but not reversal conditioning). These results demonstrate that NR neurotransmission is critical for CFTP learning. Furthermore, enhancement of CFTP learning by DCS suggests that endogenous levels of glycine or D-serine may be a limiting factor in CFTP learning.

show abstract

“…Early olfactory associative learning involves N ‐methyl‐ d ‐aspartate (NMDA)‐receptor‐dependent processes (Lincoln et al 1988; Weldon et al 1997) and is mediated by cAMP response element‐binding protein (CREB) phosphorylation (Cui et al 2007; McLean et al 1999; Raineki et al 2009; Yuan et al 2003). In adults, NMDA receptors mediate olfactory learning at multiple levels in diverse species, including Drosophila (Hudson & Distel 1986), honeybees (Si et al 2004), rats (Tronel & Sara 2003) and mice (Brennan 1994).…”

Section: Discussionmentioning

confidence: 99%

ImprintedRasgrf1expression in neonatal mice affects olfactory learning and memory

Drake

DeVito

Cleland

et al. 2011

Genes, Brain and Behavior

View full text Add to dashboard Cite

Rasgrf1 is genomically imprinted; only the paternally inherited allele is expressed in the neonatal mouse brain until weaning, at which time expression becomes biallelic. Whereas Rasgrf1 has been implicated in learning and memory via knockout studies in adult mice, the effect of its normal imprinted expression on these phenotypes has not yet been examined. Neonatal mice with experimentally manipulated patterns of imprinted Rasgrf1 expression were assessed on an associative olfactory task. Neonates lacking the normally expressed wild-type paternal allele exhibited significant impairment in olfactory associative memory. Adult animals in which neonatal imprinting had been manipulated were also behaviorally assessed; while neonatal imprinting significantly affects body weight even into adulthood, no learning and memory phenotype attributable to imprinting was observed in adults. Additional analyses of neonates showed imprinted Rasgrf1 transcript selective to olfactory bulb even in mice that were null for Rasgrf1 in the rest of the brain and showed that Rasgrf1 affects Ras and Rac activation in the brain. Taken together, these results indicate that Rasgrf1 expression from the wild-type paternal allele contributes to learning and memory in neonatal mice.

show abstract

Olfactory Conditioning Impairment Following Posttraining NMDA Receptor Blockade in Neonatal Rats

Cited by 16 publications

References 0 publications

Protein degradation by ubiquitin–proteasome system in formation and labilization of contextual conditioning memory

Protein degradation by ubiquitin–proteasome system in formation and labilization of contextual conditioning memory

NMDA receptor in conditioned flavor-taste preference learning: Blockade by MK-801 and enhancement by D-cycloserine

ImprintedRasgrf1expression in neonatal mice affects olfactory learning and memory

Contact Info

Product

Resources

About