Olfactory coding of intra- and interspecific pheromonal messages by the male Mythimna separata in North China

Jiang, Nan; Tang, Rui; Guo, Hao; Ning, Chao; Li, Jiancheng; Wu, Han; Huang, Ling-Qiao; Wang, Chen‐Zhu

doi:10.1016/j.ibmb.2020.103439

Cited by 16 publications

(43 citation statements)

References 73 publications

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“…Of those, ORN-B housed in type-I TS is considered the neuron type detecting the major pheromone component and represents the most frequently occuring neuron type in our recordings. This result is consistent with a recent study by Jiang et al (2020) . Otherwise, their previous work indicated that MsepOR3 (equal to MsepPR3 in this study, see Supplementary Figure 4 ) was tuned to the major component Z11–16:Ald and analog Z9–14:Ald from M. separata , inferring that an MsepOR3 -expressing neuron may be ORN-B of type-I TS based on identical function and the axons from this neuron projects to the cumulus (CU) of the MGC in male antennal lobes (ALs; Figure 6 ).…”

Section: Discussionsupporting

confidence: 94%

“…In our study, the response profiles of neurons of type-II TS are quite similar with those in B type sensilla reported by Jiang et al (2020) . However, the number of active neurons between these two studies is different.…”

Section: Discussionsupporting

confidence: 89%

“…Further functional characterization showed that Z9–14:Ald was recognized by HarmOR14b, HarmOR16, and HarmOR6 expressed in Type B or Type C TS ( Liu et al, 2013 ; Chang et al, 2016 ; Wang et al, 2018 ). In this study, we found that analog Z9–14:Ald could strongly elicit the ORN-B of type-II TS of M. separata , and also ORN-B of type-I TS with mild sensitivity ( Figure 6 ), corresponding to a recent study that Z9–14:Ald activated the CU and the dorso-anterior (DA) of the MGC in ALs of male M. separata ( Jiang et al, 2020 ). Wind tunnel assay further indicated that addition of Z9–14:Ald at the ratio of 1:1, 1:10, and 1:100 greatly reduced the attractiveness of M. separata to Z11–16:Ald ( Jiang et al, 2020 ), suggesting that Z9–14:Ald plays vital roles in species isolation of M. separata and its function as antagonist within noctuid moths is conserved in the evolution.…”

Section: Discussionsupporting

confidence: 88%

“…Functions of ORNs housed in each type of TS were characterized using the SSR technique. Unlike the results of two types of TS identified by Jiang et al (2020) , we characterized four functional types of TS housed 12 ORNs in M. separata , implying that peripheral coding in olfaction of M. separata was more complicated than in other Lepidoptera moths such as H. armigera and closely related species ( Wu et al, 2013 ; Zhang et al, 2013 ; Sakurai et al, 2014 ; Chang et al, 2016 ; Liu et al, 2018 ). Our results show that five ORNs are separately activated by major and minor sex pheromone components and their analogs ( Figure 3 ).…”

Section: Discussioncontrasting

confidence: 87%

“…Four functional types of TS were characterized. The classification of neurons housed in type-II TS is modified by our SSR recording and the reported results by Jiang et al (2020) . Neuron of type-II TS activated by Z11–16:OAc is divided as a active one.…”

Section: Discussionmentioning

confidence: 99%

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Functional Characterization of Sex Pheromone Neurons and Receptors in the Armyworm, Mythimna separata (Walker)

Wang

2021

Front. Neuroanat.

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Pheromone receptors (PRs) of moths are expressed on the dendritic membrane of odorant receptor neurons (ORNs) housed in the long trichoid sensilla (TS) of antennae and are essential to sex pheromone reception. The function of peripheral neurons of Mythimna separata in recognizing sex pheromones is still unclear. In this study, electroantennogram recordings were performed from male and female antennae of M. separata, and showed that the major component of sex pheromones, (Z)-11-hexadecenal (Z11–16:Ald), evoked the strongest response of male antennae with significant differences between sexes. Single sensillum recording was used to record responses of neurons housed in TS of male M. separata. The results revealed four types of TS with three neurons housed in each type, based on profiles of responses to sex pheromone components and pheromone analogs. ORN-B of type-I TS was specifically tuned to the major sex pheromone component Z11–16:Ald; ORN-Bs in type-III and type-IV TSs were, respectively, activated by minor components (Z)-11-hexadecen-1-yl acetate (Z11–16:OAc) and hexadecenal (16:Ald); and ORNs in type-II TS were mainly activated by the sex pheromone analogs. We further cloned full-length sequences of six putative PR genes and an Orco gene. Functional characterization of PRs in the Xenopus oocyte system demonstrated that male antennae-biased MsepPR1 responded strongly to (Z)-9-tetradecenal (Z9-14:Ald), suggesting that MsepPR1 may be expressed in type-II TS. MsepPR6 was exclusively tuned to (Z)-9-tetradecen-1-yl acetate (Z9–14:OAc). MsepPR2 and MsepPR4 showed no responses to any tested components. Female antennae-biased MespPR5 was broadly tuned to Z9–14:Ald, Z9–14:OAc, Z11–16:Ald, and (Z)-11-hexadecen-1-ol (Z11–16:OH). Our results further enriched the sex pheromone recognition mechanism in the peripheral nervous system of moth M. separata.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 88%

Section: Discussioncontrasting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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Functional Characterization of Sex Pheromone Neurons and Receptors in the Armyworm, Mythimna separata (Walker)

Wang

2021

Front. Neuroanat.

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Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda

Guo

Gong

et al. 2022

Pest Management Science

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BACKGROUND The fall armyworm, Spodoptera frugiperda (J. E. Smith), is a polyphagous moth species that is spreading all around the globe. It uses (Z)‐9‐tetradecenyl acetate (Z9‐14:Ac) and (Z)‐7‐dodecenyl acetate (Z7‐12:Ac) (100:3.9) as essential sex pheromone components. However, our understanding of the molecular basis of pheromone detection of S. frugiperda is still incomplete. RESULTS Herein, we identified six PRs, i.e. SfruOR6, 11, 13, 16, 56, and 62, by transcriptome sequencing. Subsequently, we heterologously expressed them in Drosophila OR67d neurons and determined their response spectra with a large panel of sex pheromones and analogs. Among them, SfruOR13‐expressing neurons strongly respond to the major sex pheromone component Z9‐14:Ac, but also comparably to (Z,E)‐9,12‐tetradecadienyl acetate (Z9,E12‐14:Ac) and weakly to (Z)‐9‐dodecenyl acetate (Z9‐12:Ac). Both SfruOR56 and SfruOR62 are specifically tuned to the minor sex pheromone component Z7‐12:Ac with varying intensities and sensitivities. In addition, SfruOR6 is activated only by Z9,E12‐14:Ac, and SfruOR16 by both (Z)‐9‐tetradecenol (Z9‐14:OH) and (Z)‐9‐tetradecenal (Z9‐14:Ald). However, the OR67d neurons expressing SfruOR11 remain silent to all compounds tested, a phenomenon commonly found in the OR11 clade of Noctuidae species. Next, using single sensillum recording, we characterized four sensilla types on the antennae of males, namely A, B, C and D types that are tuned to the ligands of PRs, thereby confirming that S. frugiperda uses both SfruOR56 and SfruOR62 to detect Z7‐12:Ac. Finally, using wind tunnel assay, we demonstrate that both Z9,E12‐14:Ac and Z9‐14:OH act as antagonists to the sex pheromone. CONCLUSION We have deorphanized five PRs and characterized four types of sensilla responsible for the detection of pheromone compounds, providing insights into the peripheral encoding of sex pheromones in S. frugiperda.

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The Mythimna separata general odorant binding protein 2 (MsepGOBP2) is involved in the larval detection of the sex pheromone (Z)‐11‐hexadecenal

Tian

Cheng

et al. 2023

Pest Management Science

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BACKGROUND: Mythimna separata is a notorious pest causing crop damages at the larval stage. Gaining insight into larval olfaction mechanisms would provide knowledge for olfaction-based management of M. separata larvae. RESULTS:In the present research, (Z)-11-hexadecenal (Z11-16: Ald), a major component of M. separata sex pheromone, was found to attract early-instar larvae of M. separata in a food context. Using a fluorescent binding assay, we found that M. separata general odorant binding protein 2 (MsepGOBP2) exhibited high binding affinity to Z11-16: Ald. Further, silencing of MsepGOBP2 resulted in a sharp reduction of the response to Z11-16: Ald, which could not be mitigated by increasing the concentration of Z11-16: Ald. Additionally, we employed molecular dynamics-based approaches to unravel the interaction details between MsepGOBP2 and Z11-16: Ald, specifically the binding of Z11-16: Ald to MsepGOBP2. CONCLUSION: Z11-16: Ald is attractive to early-instar larvae of M. separata, and MsepGOBP2 is identified to be indispensable in the larval detection of Z11-16: Ald. These results could aid in the development of olfaction-based methods for controlling M. separata in the larval stage.

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Olfactory coding of intra- and interspecific pheromonal messages by the male Mythimna separata in North China

Cited by 16 publications

References 73 publications

Functional Characterization of Sex Pheromone Neurons and Receptors in the Armyworm, Mythimna separata (Walker)

Functional Characterization of Sex Pheromone Neurons and Receptors in the Armyworm, Mythimna separata (Walker)

Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda

The Mythimna separata general odorant binding protein 2 (MsepGOBP2) is involved in the larval detection of the sex pheromone (Z)‐11‐hexadecenal

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