Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats

Yin, Min; Jiang, Nan; Guo, Li; Ni, Ziyuan; Albrakati, Ashraf; Othman, Mohamed S.; Moneim, Ahmed E. Abdel; Kassab, Rami B.

doi:10.1016/j.lfs.2019.116634

Cited by 44 publications

(43 citation statements)

References 54 publications

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“…Our results support other studies that have shown remarkable increases in lipid and protein degradation in all target organs of septic rats. 27 It has been reported that OLE has the ability to decrease MDA level 24,28 as well as lipid peroxidation in healthy individuals. 29 We found that OLE pretreatment decreased MDA levels in septic mice.…”

Section: Discussionmentioning

confidence: 99%

“…Oleuropein has been recorded to have valuable cardioprotective, antioxidant, 22,23 and anti-inflammatory effects. 24 Sepsis can induce disorders in many organs and lead to death. 8,25 The most vital organs are the liver and kidney.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, oleuropein has been declared to have potent antioxidant properties and can reduce renal injury by scavenging the reactive oxygen species (ROS) and enhancing the renal antioxidant system. 24 Neutrophil accumulation occurs in the liver during early sepsis development. This accumulation leads to the release of proteolytic enzymes and ROS.…”

Section: Discussionmentioning

confidence: 99%

“…It was demonstrated that OLE might act as a chainbreaking antioxidant, leading to termination of the chain reaction of lipid peroxidation. 24 Another study has revealed that OLE possesses two hydroxyl functions (orthodiphenol group). In this context, OLE has the ability to induce the oxidation (non-enzymatic) of superoxide anion to molecular oxygen and dismutase superoxide radicals.…”

Section: Discussionmentioning

confidence: 99%

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Oleuropein protects against lipopolysaccharide‐induced sepsis and alleviates inflammatory responses in mice

et al. 2020

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Sepsis results from a major systemic inflammatory response and can induce disorders in multiple organs. The present study evaluated the potential protective effects of oleuropein (OLE) against hyperinflammatory responses during lipopolysaccharide (LPS)‐induced sepsis in mice. Sixty male Balb/c mice were randomly categorized into five groups of 12 animals each: control, intraperitoneally injected with OLE (50 mg/kg), injected with LPS (10 mg/kg, intraperitoneal), and two groups administered OLE (25 and 50 mg/kg) for 3 days prior to LPS injection. Twenty‐four hours after lipopolysaccharide injection, the animals were sacrificed. Serum, liver, and kidney tissue samples were collected for biochemical analyses, histopathological examinations, and investigation of inflammation‐related gene expression. OLE pretreatment significantly reduced liver damage parameters (alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase) and kidney damage parameters (blood urea nitrogen, creatinine, and kidney injury molecule‐1) in the septic mice. OLE pretreatment ameliorated LPS‐induced liver and kidney histological changes. OLE significantly mitigated the increased levels of malondialdehyde in the liver and kidneys and reduced levels of reduced glutathione induced by LPS. LPS injection also resulted in increased expression of the proinflammatory cytokines (TNF‐α, IL‐1β, and IL‐6) and inflammation‐related genes (Nos2, Hmgb1, Mpo, Cd46, Map2k4, and Map2k7) in the hepatic and renal tissues. OLE reduced these expressions to ameliorate the inflammatory response. Moreover, OLE pretreatment enhanced the survival rate of septic mice. In conclusion, OLE alleviated the inflammatory response to protect against LPS‐induced sepsis in mice.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Oleuropein protects against lipopolysaccharide‐induced sepsis and alleviates inflammatory responses in mice

et al. 2020

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“…As expected, BBR administration reversed the disturbance in the oxidative stress index induced by HgCl 2 , as indicated by the inhibition of LPO and NO production, along with the increased content and activity of antioxidant molecules and their gene expression in the testicular tissue. These findings suggest that antioxidant agents may inhibit LPO‐induced oxidative insult and the subsequent exacerbation of impairments associated with HgCl 2 ‐mediated testicular damage (Yin et al, 2019). BBR was shown to enhance the antioxidant response through scavenging ROS, preventing of LPO, and increasing gene and protein expression in the antioxidant defense system in different experimental models (Hassani‐Bafrani et al, 2019; Kazaz et al, 2019; Saleh et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Berberine mitigates oxidative damage associated with testicular impairment following mercury chloride intoxication

2020

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In this study, we assessed the protective effect of berberine (BBR) against mercuric chloride (HgCl2)‐induced reproductive impairment. Twenty‐eight adult male Wistar albino rats were placed into four equal groups: control, BBR, HgCl2, and BBR + HgCl2. All the treatments were orally administered for seven consecutive days. The rats exposed to HgCl2 showed a marked decrease in testosterone accompanied by an increase in lipid peroxidation (LPO) and nitric oxide (NO). Additionally, HgCl2 decreased glutathione (GSH) content, deactivated catalase (CAT) and glutathione reductase (GR), and suppressed the activities and mRNA expression of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the testicular tissue. In addition, histological deformations and testicular cell loss were recorded, as evidenced by the upregulation of caspase‐3 following HgCl2 intoxication. Notably, BBR administration reversed the testicular impairments associated with HgCl2 exposure. These findings suggest that BBR protected the testicular tissue following HgCl2 exposure through inhibiting the disturbance in the testosterone level and enhanced the antioxidant capacity. Practical applications Heavy metals are naturally existing metallic elements in the earth’s crust. These trace metals have the potential to cause multiple adverse reactions to the living systems, even at low exposure doses. Human exposure may also result from industrial, agricultural, and domestic activities. Berberine (BBR, a naturally occurring quaternary benzylisoquinoline alkaloid) is a protoberberine and is the representative main active ingredient in all parts of Berberis species. BBR has been used widely in folk medicine worldwide for its multiple pharmacological and therapeutic activities; for example, in the treatment of digestive and reproductive system disorders, microorganism infections, hemorrhage and wound healing, and cardiovascular and ophthalmic diseases. We found that BBR administration was able to abrogate significantly the reproductive toxicity associated with Hg intoxication. This protective effect comes from its strong antioxidant, anti‐inflammatory, and antiapoptotic activities; suggesting that BBR may be applied to alleviate reproductive toxicity associated with Hg intoxication.

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Stevioside protects against rhabdomyolysis‐induced acute kidney injury through PPAR‐γ agonism in rats

Kaur

Singh

et al. 2020

Drug Development Research

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We explored the potential role of peroxisome proliferator activated receptor‐γ (PPAR‐γ) in stevioside‐mediated renoprotection using rhabdomyolysis‐induced acute kidney injury (AKI) model in rats. Rhabdomyolysis refers to intense skeletal muscle damage, which further causes AKI. Glycerol (50% w/v, 8 ml/kg) was injected intramuscularly in rats to induce rhabdomyolysis. After 24 hr, AKI was demonstrated by quantifying serum creatinine, urea, creatinine clearance, microproteinuria, and electrolytes in rats. Further, oxidative stress was measured by assaying thiobarbituric acid reactive substances, generation of superoxide anion, and reduced glutathione levels. Additionally, serum creatine kinase (CK) level was assayed to determine glycerol‐induced muscle damage in rats. Pathological changes in rat kidneys were studied using hematoxylin–eosin and periodic acid Schiff staining. Moreover, the expression of apoptotic markers (Bcl‐2, Bax) in rat kidneys was demonstrated by immunohistochemistry. Stevioside (10, 25, and 50 mg/kg) was administered to rats, prior to the induction of AKI. In a separate group, bisphenol A diglycidyl ether (BADGE, 30 mg/kg), a PPAR‐γ receptor antagonist was given prior to stevioside administration, which was followed by rhabdomyolysis‐induced AKI in rats. The significant alteration in biochemical and histological parameters in rats indicated AKI, which was attenuated by stevioside treatment. Pretreatment with BADGE abrogated stevioside‐mediated renoprotection, which is suggestive of the involvement of PPAR‐γ in its renoprotective effect. In conclusion, stevioside protects against rhabdomyolysis‐induced AKI, which may be attributed to modulation of PPAR‐γ expression.

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Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats

Cited by 44 publications

References 54 publications

Oleuropein protects against lipopolysaccharide‐induced sepsis and alleviates inflammatory responses in mice

Oleuropein protects against lipopolysaccharide‐induced sepsis and alleviates inflammatory responses in mice

Berberine mitigates oxidative damage associated with testicular impairment following mercury chloride intoxication

Stevioside protects against rhabdomyolysis‐induced acute kidney injury through PPAR‐γ agonism in rats

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