Oleuropein Suppresses LPS-Induced Inflammatory Responses in RAW 264.7 Cell and Zebrafish

Ryu, Su-Jung; Choi, Hyeon‐Son; Yoon, Kye-Yoon; Lee, Ok‐Hwan; Kim, Kui-Jin; Lee, Boo-Yong

doi:10.1021/jf505894b

Cited by 96 publications

(60 citation statements)

References 35 publications

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“…NF-κB resides inactive within the cytoplasm due to the presence of IκB kinase, an inhibitor enzyme, therefore it can be activated very quickly to initiate cytokine and prostanoid production. There is strong evidence that olive polyphenols are able to interact with these pathways [119,120,121]. …”

Section: Olive Leaf Properties That Protect Against Development Anmentioning

confidence: 99%

“…Cellular studies with OLE polyphenols have found a protective effect in relation to inflammation; a down-regulation of NO and COX-2 [120,123,124,125]. Inhibition of the Toll-like receptor (TLR) signaling induced by LPS was demonstrated not only by down-regulation of iNOS and COX2, but also by a decrease in ERK1/2, JNK and nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα) phosphorylation in vitro after oleuropein treatment [120] (Figure 5). In down-regulating this pathway the pro-inflammatory enzymes interleukin 6 (IL-6) and interleukin 1β (IL-1β) and the gene AP-1 were also down-regulated.…”

Section: Olive Leaf Properties That Protect Against Development Anmentioning

confidence: 99%

“…This would explain the observed gene expression in in vitro models [46,120,126]. A recent study has indicated olive leaf polyphenols in a quinone form could interact with Topoisomerase IIα [149].…”

Section: Olive Leaf Properties That Protect Against Development Anmentioning

confidence: 99%

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Evidence to Support the Anti-Cancer Effect of Olive Leaf Extract and Future Directions

et al. 2016

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The traditional Mediterranean diet (MD) is associated with long life and lower prevalence of cardiovascular disease and cancers. The main components of this diet include high intake of fruit, vegetables, red wine, extra virgin olive oil (EVOO) and fish, low intake of dairy and red meat. Olive oil has gained support as a key effector of health benefits and there is evidence that this relates to the polyphenol content. Olive leaf extract (OLE) contains a higher quantity and variety of polyphenols than those found in EVOO. There are also important structural differences between polyphenols from olive leaf and those from olive fruit that may improve the capacity of OLE to enhance health outcomes. Olive polyphenols have been claimed to play an important protective role in cancer and other inflammation-related diseases. Both inflammatory and cancer cell models have shown that olive leaf polyphenols are anti-inflammatory and protect against DNA damage initiated by free radicals. The various bioactive properties of olive leaf polyphenols are a plausible explanation for the inhibition of progression and development of cancers. The pathways and signaling cascades manipulated include the NF-κB inflammatory response and the oxidative stress response, but the effects of these bioactive components may also result from their action as a phytoestrogen. Due to the similar structure of the olive polyphenols to oestrogens, these have been hypothesized to interact with oestrogen receptors, thereby reducing the prevalence and progression of hormone related cancers. Evidence for the protective effect of olive polyphenols for cancer in humans remains anecdotal and clinical trials are required to substantiate these claims idea. This review aims to amalgamate the current literature regarding bioavailability and mechanisms involved in the potential anti-cancer action of olive leaf polyphenols.

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Section: Olive Leaf Properties That Protect Against Development Anmentioning

confidence: 99%

Section: Olive Leaf Properties That Protect Against Development Anmentioning

confidence: 99%

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Evidence to Support the Anti-Cancer Effect of Olive Leaf Extract and Future Directions

et al. 2016

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“…In vitro and animal studies suggest that the health benefits of OLE are due, at least in part, to OLE polyphenols influencing gene expression [1,2]. In vitro studies using human cell lines have suggested that OLE has anti-inflammatory [3] and anti-cancer effects [2]. In vivo animal studies suggest that OLE attenuates obesity from a high fat diet [4] and reduces impacts resulting from inflammatory cytokine production and insulin resistance [5].…”

Section: Introductionmentioning

confidence: 99%

Human Intervention Study to Assess the Effects of Supplementation with Olive Leaf Extract on Peripheral Blood Mononuclear Cell Gene Expression

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Kao

Murray

et al. 2016

IJMS

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Olive leaf extract (OLE) has been used for many years for its putative health benefits, but, to date, scientific evidence for the basis of these effects has been weak. Although recent literature has described a link between ailments such as cardiovascular disease, diabetes and cancer and a protective effect of polyphenols in the OLE, the mode of action is still unclear. Here, we describe a double-blinded placebo (PBO)-controlled trial, in which gene expression profiles of peripheral blood mononuclear cells from healthy male volunteers (n = 29) were analysed to identify genes that responded to OLE, following an eight-week intervention with 20 mL daily consumption of either OLE or PBO. Differences between groups were determined using an adjusted linear model. Subsequent analyses indicated downregulation of genes important in inflammatory pathways, lipid metabolism and cancer as a result of OLE consumption. Gene expression was verified by real-time PCR for three genes (EGR1, COX-2 and ID3). The results presented here suggest that OLE consumption may result in health benefits through influencing the expression of genes in inflammatory and metabolic pathways. Future studies with a larger study group, including male and female participants, looking into direct effects of OLE on lipid metabolism and inflammation are warranted.

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“…We performed the experiments like our previous paper [25]. All zebrafish experiments were approved by the Internal Animal Ethics Committee at CHA University (IACUC140001).…”

Section: Zebrafish Care and Experimental Protocolmentioning

confidence: 99%

Compound K Inhibits the Lipopolysaccharide-Induced Inflammatory Responses in Raw 264.7 Cell Line and Zebrafish

et al. 2018

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Compound K, a major metabolite of ginsenosides Rb1, which is produced by human intestinal bacteria after oral administration, is one of the main pharmacologic compounds found in ginseng. In our previous study, we demonstrated that compound K inhibited the production of nitric oxide (NO) and prostaglandin E2 in lipopolysaccharide (LPS)-treated RAW264.7 cells. However, the mechanisms by which compound K may be effective against inflammation remain unknown. In the present study, compound K significantly inhibited LPS-induced NO production by suppression of inducible NO synthase (iNOS) in LPS-treated RAW264.7 cells. Compound K also inhibited LPS-induced cyclooxygenase-2 (COX-2) expression at both the mRNA and protein levels. It effectively suppressed both the release and mRNA expression levels of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and IL-6. The anti-inflammatory effects of compound K appeared to occur via inhibition of LPS-induced phosphorylation of mitogen-activated protein kinases (MAPKs) and inhibition of NF-κB translocation from the cytosol to the nucleus by suppressing phosphorylation of inhibitory kappa B-α (IκB-α). Furthermore, we showed that compound K inhibited LPS-induced NO generation in an experimental zebrafish model. Considering these results, compound K could potentially be developed as a natural anti-inflammatory agent.

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Oleuropein Suppresses LPS-Induced Inflammatory Responses in RAW 264.7 Cell and Zebrafish

Cited by 96 publications

References 35 publications

Evidence to Support the Anti-Cancer Effect of Olive Leaf Extract and Future Directions

Evidence to Support the Anti-Cancer Effect of Olive Leaf Extract and Future Directions

Human Intervention Study to Assess the Effects of Supplementation with Olive Leaf Extract on Peripheral Blood Mononuclear Cell Gene Expression

Compound K Inhibits the Lipopolysaccharide-Induced Inflammatory Responses in Raw 264.7 Cell Line and Zebrafish

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