Oleo gum resin of Ferula assa-foetida L. ameliorates peripheral neuropathy in mice

Moghadam, Farshad Homayouni; Dehghan, Mohammad Ali; Zarepur, Ehsan; Dehlavi, Reyhaneh; Ghaseminia, Fatemeh; Ehsani, Shima; Mohammadzadeh, Golnaz; Barzegar, Kazem

doi:10.1016/j.jep.2014.03.069

Cited by 34 publications

(13 citation statements)

References 30 publications

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“…Oleo-gum resin of Ferula assa-foetida L. (asafetida) was prepared based on our previous study [38]. In our experiments, we used a special, pure type of asafetida (Ashki asafetida in local dialect) that, based on high performance liquid chromatography (HPLC) assays, has a higher concentration of FA [38].…”

Section: Methodsmentioning

confidence: 99%

“…In our experiments, we used a special, pure type of asafetida (Ashki asafetida in local dialect) that, based on high performance liquid chromatography (HPLC) assays, has a higher concentration of FA [38]. Briefly, Oleo-gum resin was collected in June from Ferula assa-foetida L. (Herbarium code: P1006636, IBRC, Tehran, Iran) by making some small incisions (1 – 5 cm) on its stem near to its roots, from which high-quality oleo-gum resin (asafetida) could be obtained.…”

Section: Methodsmentioning

confidence: 99%

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Effects of Oleo Gum Resin of Ferula assa-foetida L. on Senescence in Human Dermal Fibroblasts

2017

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ObjectivesBased on data from Chinese and Indian traditional herbal medicines, gum resin of Ferula assa-foetida (sometimes referred to asafetida or asafoetida) has several therapeutic applications. The authors of various studies have claimed that asafetida has cytotoxic, antiulcer, anti-neoplasm, anti-cancer, and anti-oxidative effects. In present study, the anti-aging effect of asafetida on senescent human dermal fibroblasts was evaluated.MethodsSenescence was induced in in vitro cultured human dermal fibroblasts (HDFs) through exposure to H2O2, and the incidence of senescence was recognized by using cytochemical staining for the activity of β-galactosidase. Then, treatment with oleo gum resin of asafetida was started to evaluate its rejuvenating effect. The survival rate of fibroblasts was evaluated by using methyl tetrazolium bromide (MTT) assays. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blot assays were performed to evaluate the expressions of apoptotic and anti-apoptotic markers.ResultsOur experiments show that asafetida in concentrations ranging from 5 × 10−8 to 10−7 g/mL has revitalizing effects on senescent fibroblasts and significantly reduces the β-galactosidase activity in these cells (P < 0.05). Likewise, treatment at these concentrations increases the proliferation rate of normal fibroblasts (P < 0.05). However, at concentrations higher than 5 × 10−7 g/mL, asafetida is toxic for cells and induces cell death.ConclusionThe results of this study indicate that asafetida at low concentrations has a rejuvenating effect on senescent fibroblasts whereas at higher concentrations, it has the opposite effect of facilitating cellular apoptosis and death.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of Oleo Gum Resin of Ferula assa-foetida L. on Senescence in Human Dermal Fibroblasts

2017

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“…In both tests, FAEE showed a significant increase in SOD and CAT levels, compared with the depression-induced (positive control) animals [35]. This effect may be due the presence of phytochemical constituents like 'Ferulic acid and umbellifedrone', which has already been reported [36] It may also be due to the neuroprotective activity of other phytoconstituents, such as flavonoids, phenolic acids and polysulfide compounds [37].…”

Section: Discussionmentioning

confidence: 75%

“…It is also used in the management of depression, but its neuropharmacological mechanism on antidepressant-like action is unknown. FA was found to have potent antioxidant activity [23] and neuroprotective activity [24] which has been proved. Based on this evidence, i.e., effectiveness against the ROS which is one of the major causes of depression, as well as neuroprotective activity, initiated the development of an alternative medicine from a herbal source.…”

Section: Discussionmentioning

confidence: 99%

Antidepressant activity of ethanolic extract of oleo gum resins of Ferula asafoetida Linn

Kumar¹,

Reddy²,

Rushendran³

et al. 2017

J Pre Clin Clin Res.

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Objective. The study was undertaken to evaluate the antidepressant activity of ethanolic extract of Ferula asafoetida oleo gum resins. Materials and Methods. Five groups of rats (180-200g) and mice (20-30g) of both genders, each group comprising six animals, were used (i.e., normal, positive control, standard, FAEE 200mg/kg, and FAEE 400mg/kg treated groups). Forced swimming test (FST), Tail suspension test (TST), Potentiation of Norepinephrine-Induced Toxicity (PNEIT), Haloperidol-Induced Catalepsy (HIC), and Reserpine-Induced Hypothermia (RIH), were used as the validate models of depression in rodents. The study was confirmed by brain monoamines estimation (i.e. Dopamine, Norepinephrine and 5-HT), MAO levels and invivo antioxidant studies (CAT and SOD). Results. FAEE treated animals showed a significant and dose dependent effect on a decrease in immobility time in FST, TST, and decrease in catalepsy time in HIC. FAEE and imipramine (15mg/kg) showed a significant increase in body temperature in RIH, and also showed a potent lethality in PNEIT. FAEE treated animals showed a significant increase in the levels of brain monoamines, in vivo antioxidants, and a significant decrease in MAO levels. Conclusion. Results of present study indicate that FAEE has potent antidepressant-like activity, and this effect may be due to the anti-oxidant property of Ferulic acid and umbelliferone, or may be due to neuroprotective activity of other major phytoconstituents, e.g. flavonoids, phenolic acids and polysulfide compounds. To identify the particular compound responsible for the antidepressant-like activity required further molecular level studies.

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“…In the folklore medicine, it is mostly used in asthma, gastrointestinal disorders, and neuronal disorders [ 20 ]. In recent reports, the resin improved memory and learning in rats [ 21 ], and exhibited neuroprotection and nerve stimulation in mice peripheral neuropathy [ 22 ], and anticonvulsant properties [ 23 ]. FAR contains bioactive phytochemicals such as polysulfides, sesquiterpenes, sesquiterpene-coumarins, diterpenes, phenolics, and flavonoids [ 20 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Natural Products Screening for the Identification of Selective Monoamine Oxidase-B Inhibitors

Zarmouh

Messeha

Elshami

et al. 2016

EJMP

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Aims Monoamine oxidase-B inhibitors (MAO-BIs) are used for the initial therapy of Parkinson’s disease. Also, MAO-BIs have shown to be effective neuroprotective agents in several neurodegenerative diseases. However, some concerns exist regarding the long-term use of these compounds. Meanwhile, natural compounds showed potential MAO-B selective inhibitions. To date, few selective natural MAO-BIs have been identified. Therefore, the current study is designed to identify plants with potent and specific MAO-B inhibition. Study Design In this work, we utilized high throughput screening to evaluate the different plants ethanolic extract for their effectiveness to inhibit recombinant human (h)MAO-A and hMAO-B and to determine the relative selectivity of the top MAO-BI. Methodology Recombinant human isozymes were verified by Western blotting, and the 155 plants were screened. A continuous fluorometric screening assay was performed followed by two separate hMAO-A and hMAO-B microtiter screenings and IC50 determinations for the top extracts. Results In the screened plants, 9% of the extracts showed more than 1.5-fold relative inhibition of hMAO-B (RIB) and another 9% showed more than 1.5-fold relative inhibition of hMAO-A. The top extracts with the most potent RIBs were Psoralea corylifolia seeds, Phellodendron amurense bark, Glycyrrhiza uralensis roots, and Ferula assafoetida roots, with the highest RIB of 5.9-fold. Furthermore, extensive maceration of the promising extracts led to increase inhibitory effects with a preserved RIB as confirmed with luminescence assay. The top four extracts hMAO-BIs were equally potent (IC50= 1.3 to 3.8 μg/mL) with highly significant relative selectivities to inhibit hMAO-B (4.1- to 13.4-fold). Conclusion The obtained results indicate that Psoralea corylifolia seeds, Ferula assafoetida, Glycyrrhiza uralensis roots, and Phellodendron amurense ethanolic extracts have selective inhibitions for human MAO-B. Investigating these plant extracts as natural resources for novel selective MAO-BIs may lead to the development of molecules that can be used in the therapeutic management of neurodegenerative diseases including Parkinson’s disease.

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Oleo gum resin of Ferula assa-foetida L. ameliorates peripheral neuropathy in mice

Cited by 34 publications

References 30 publications

Effects of Oleo Gum Resin of Ferula assa-foetida L. on Senescence in Human Dermal Fibroblasts

Effects of Oleo Gum Resin of Ferula assa-foetida L. on Senescence in Human Dermal Fibroblasts

Antidepressant activity of ethanolic extract of oleo gum resins of Ferula asafoetida Linn

Natural Products Screening for the Identification of Selective Monoamine Oxidase-B Inhibitors

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