Oleic acid reduces lipopolysaccharide-induced expression of iNOS and COX-2 in BV2 murine microglial cells: Possible involvement of reactive oxygen species, p38 MAPK, and IKK/NF-κB signaling pathways

Oh, Young Taek; Lee, Jung Yeon; Lee, Jinhwa; Kim, Ho-Cheol; Yoon, Kyung‐Sik; Choe, Wonchae; Kang, Insug

doi:10.1016/j.neulet.2009.08.040

Cited by 105 publications

(83 citation statements)

References 28 publications

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“…Ambrozova et al (2010) [40] reported that LA can inhibit LPS-induced nitrite accumulation and suggested that UFAs may exhibit scavenging activity against NO production. Consistent with the results reported by Ambrozova et al [41], we demonstrated that both the production of NO and the expression of iNOS by LPS were markedly reduced in RAW264.7 cells after pre-treatment with UFA-BSA (i.e., OA and LA), but not with SFA-BSA (i.e., PA and SA) (Fig. 1a, b).…”

Section: Discussionsupporting

confidence: 92%

The lipopolysaccharide-induced pro-inflammatory response in RAW264.7 cells is attenuated by an unsaturated fatty acid–bovine serum albumin complex and enhanced by a saturated fatty acid–bovine serum albumin complex

et al. 2011

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Section: Discussionsupporting

confidence: 92%

The lipopolysaccharide-induced pro-inflammatory response in RAW264.7 cells is attenuated by an unsaturated fatty acid–bovine serum albumin complex and enhanced by a saturated fatty acid–bovine serum albumin complex

et al. 2011

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“…Phosphorylation of c-Jun and ATF2 by JNK and p38 increase the transcriptional activity of AP-1, leading to expression of inflammatory cytokines [26]. The effects of LPS on microglia have been previously investigated [57,58]. In BV2 microglia, LPS-induced activation involves iNOS expression, NFκB activation, p38 phosphorylation, reactive oxygen species production and cyclooxygenase − 2 expression [57].…”

Section: Discussionmentioning

confidence: 99%

“…The effects of LPS on microglia have been previously investigated [57,58]. In BV2 microglia, LPS-induced activation involves iNOS expression, NFκB activation, p38 phosphorylation, reactive oxygen species production and cyclooxygenase − 2 expression [57]. Interestingly, OA showed an anti-inflammatory effect by reducing the proinflammatory effects of LPS.…”

Section: Discussionmentioning

confidence: 99%

Dynamics and regulation of lipid droplet formation in lipopolysaccharide (LPS)-stimulated microglia

Khatchadourian

Bourque

Richard

et al. 2012

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Lipid droplets (LDs) are neutral lipid-rich organelles involved in many cellular processes. A well-known example is their accumulation in leukocytes upon activation by pro-inflammatory stimuli such as lipopolysaccharides (LPS) derived from gram-negative bacteria. A role of LDs and LD-associated proteins during inflammation in the brain is unknown, however. We have now studied their dynamics and regulation in microglia, the resident immune cells in the brain. We find that LPS treatment of microglia leads to the accumulation in them of LDs, and enhancement of the size of LDs. This induction of LDs was abolished by triacsin C, an inhibitor of triglyceride biosynthesis. LPS strongly activated c-Jun N-terminal kinase (JNK) and p38 MAPK stress signaling pathways and increased the expression of LD-associated protein perilipin-2 (ADRP) in a time-dependent manner. Immunostaining showed that perilipin-2 in LPS-treated microglia predominantly colocalized with LDs. Inhibitors of p38 α/β (SB203580) and PI3K/Akt pathway (LY294002), but not that of JNK (SP600125), reduced LPS-induced LD accumulation and eliminated the activating effect of LPS on perilipin-2. In addition, cytosolic phospholipase A 2 (cPLA 2 -α), a key enzyme for arachidonic acid release, colocalized with LPS-induced LDs. These observations suggest that LDs may play an important role in eicosanoid synthesis in activated microglia; they provide a novel insight into the regulation of LDs in inflammatory cells of the brain and point to a potential role of p38 α/β in LPS-induced LD accumulation. Collectively, our findings imply that LD formation and perilipin-2 induction could be microglial biomarkers of inflammation in the central nervous system.

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“…Moreover, oleic acid suppresses the release of pro-NO and PGE 2 mediators, as well as the expression of iNOS and COX-2 in LPSstimulated microglial cells. This anti-inflammatory effect of oleic acid is associated with the blockade of reactive oxygen species (ROS) (Oh et al, 2009). Therefore, the importance of the oleic acid present in OEO is due to its role in the inflammatory process (Cardoso et al, 2004;Pereira et al, 2008, OH et al, 2009.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory and antinociceptive activities of Euterpe oleracea Mart., Arecaceae, oil

Favacho¹,

Oliveira²,

Santos³

et al. 2011

Rev. bras. farmacogn.

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Abstract:The oil of the fruits of Euterpe oleracea Mart., Arecaceae (OEO), was evaluated in models of inflammation and hyperalgesia in vivo to study its effects on these conditions. The experimental models contained the writhing test in mice, rat paw edema, granuloma test in rats, vascular permeability in rats, cell migration to the peritoneal cavity in rats and ear erythema induced by croton oil in mice. Doses of 500, 1000 and 1500 mg/kg of OEO were administered orally. The observed number of writhes was inhibited by 33.67, 45.88 and 55.58%, respectively. OEO produced a dose-dependent effect, with linear correlation coefficient R=0.99 (y=0.0219x+23.133), and the median effective dose found was 1226.8 mg/kg. The oral administration of 1226.8 mg/kg of OEO inhibited carrageenan-induced edema by 29.18% (p<0.05) when compared to the control group. The daily administration of OEO for six days inhibited the formation of granulomatous tissue by 36.66% (p<0.01). In ear erythema induced by croton oil, OEO presented a significant inhibition (37.9%). In the vascular permeability test, treatment with OEO decreased the response to histamine, inhibiting vascular permeability by 54.16%. In carrageenan-induced peritonitis, OEO reduced the number of neutrophils migrating compared to the control group by 80.14%. These results suggested that OEO has anti-inflammatory and antinociceptive activities, probably of peripheral origin and linked to prostaglandin biosynthesis inhibition.

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Oleic acid reduces lipopolysaccharide-induced expression of iNOS and COX-2 in BV2 murine microglial cells: Possible involvement of reactive oxygen species, p38 MAPK, and IKK/NF-κB signaling pathways

Cited by 105 publications

References 28 publications

The lipopolysaccharide-induced pro-inflammatory response in RAW264.7 cells is attenuated by an unsaturated fatty acid–bovine serum albumin complex and enhanced by a saturated fatty acid–bovine serum albumin complex

The lipopolysaccharide-induced pro-inflammatory response in RAW264.7 cells is attenuated by an unsaturated fatty acid–bovine serum albumin complex and enhanced by a saturated fatty acid–bovine serum albumin complex

Dynamics and regulation of lipid droplet formation in lipopolysaccharide (LPS)-stimulated microglia

Anti-inflammatory and antinociceptive activities of Euterpe oleracea Mart., Arecaceae, oil

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