Oleic acid promotes the expression of neural markers in differentiated human endometrial stem cells

Kojour, Maryam Ali Mohammadie; Ebrahimi‐Barough, Somayeh; Kouchesfahani, Homa Mohseni; Jalali, Hanieh; Ebrahim, Mohammah Hosein Karbalaie

doi:10.1016/j.jchemneu.2016.11.004

Cited by 12 publications

(11 citation statements)

References 32 publications

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“…Lathosterol participates in the production of cholesterol [ 48 ]. Oleic acid has anti-inflammatory and antioxidant effects, and is also related to mitochondrial function and hormone effects [ 49 , 50 ].…”

Section: Discussionmentioning

confidence: 99%

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

et al. 2021

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This study evaluated the differences in metabolites between cumulus cells (CCs) and mural granulosa cells (MGCs) from human preovulatory follicles to understand the mechanism of oocyte maturation involving CCs and MGCs. CCs and MGCs were collected from women who were undergoing in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) treatment. The differences in morphology were determined by immunofluorescence. The metabolomics of CCs and MGCs was measured by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) followed by quantitative polymerase chain reaction (qPCR) and western blot analysis to further confirm the genes and proteins involved in oocyte maturation. CCs and MGCs were cultured for 48 h in vitro, and the medium was collected for detection of hormone levels. There were minor morphological differences between CCs and MGCs. LC-MS/MS analysis showed that there were differences in 101 metabolites between CCs and MGCs: 7 metabolites were upregulated in CCs, and 94 metabolites were upregulated in MGCs. The metabolites related to cholesterol transport and estradiol production were enriched in CCs, while metabolites related to antiapoptosis were enriched in MGCs. The expression of genes and proteins involved in cholesterol transport (ABCA1, LDLR, and SCARB1) and estradiol production (SULT2B1 and CYP19A1) was significantly higher in CCs, and the expression of genes and proteins involved in antiapoptosis (CRLS1, LPCAT3, and PLA2G4A) was significantly higher in MGCs. The level of estrogen in CCs was significantly higher than that in MGCs, while the progesterone level showed no significant differences. There are differences between the metabolomes of CCs and MGCs. These differences may be involved in the regulation of oocyte maturation.

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Section: Discussionmentioning

confidence: 99%

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

et al. 2021

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“…Casado-Díaz et al have shown that arachidonic acid induces the adipogenic differentiation of MSCs while inhibiting osteoblastogenesis [54]. In addition to stimulating MSCs' motility [55], oleic acid can also stimulate the expression of neural markers in MSCs isolated from the human endometrium [56]. In contrast to the arachidonic effect, linolenic acid stimulates osteogenic and inhibits adipogenic MSCs' differentiation [57].…”

Section: Discussionmentioning

confidence: 99%

Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin

et al. 2021

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Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs >in vivo.

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“…Improved neuronal survival translates to a greater percentage of viable cells in a stressed microenvironment. There is a possibility that oleic acid could offer some neurotrophic benefit in peripheral nerve injuries, similar to axonal development in brain tissue via increasing expression of axonal growth-associated protein and microtubule-associated protein-2 [34][35][36]. In addition to preventing cellular oxidative damage, NAC acts as a neuroprotectant against cell death associated with insufficient availability of trophic factors [37,38].…”

Section: In Vitro Cytocompatibilitymentioning

confidence: 99%

Gellan-Xanthan Hydrogel Conduits with Intraluminal Electrospun Nanofibers as Physical, Chemical and Therapeutic Cues for Peripheral Nerve Repair

Ramburrun

Kumar

Ndobe

et al. 2021

IJMS

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Optimal levels of functional recovery in peripheral nerve injuries remain elusive due to the architectural complexity of the neuronal environment. Commercial nerve repair conduits lack essential guidance cues for the regenerating axons. In this study, the regenerative potential of a biosimulated nerve repair system providing three types of regenerative cues was evaluated in a 10 mm sciatic nerve-gap model over 4 weeks. A thermo-ionically crosslinked gellan-xanthan hydrogel conduit loaded with electrospun PHBV-magnesium oleate-N-acetyl-cysteine (PHBV-MgOl-NAC) nanofibers was assessed for mechanical properties, nerve growth factor (NGF) release kinetics and PC12 viability. In vivo functional recovery was based on walking track analysis, gastrocnemius muscle mass and histological analysis. As an intraluminal filler, PHBV-MgOl-NAC nanofibers improved matrix resilience, deformation and fracture of the hydrogel conduit. NGF release was sustained over 4 weeks, governed by Fickian diffusion and Case-II relaxational release for the hollow conduit and the nanofiber-loaded conduit, respectively. The intraluminal fibers supported PC12 proliferation by 49% compared to the control, preserved up to 43% muscle mass and gradually improved functional recovery. The combined elements of physical guidance (nanofibrous scaffolding), chemical cues (N-acetyl-cysteine and magnesium oleate) and therapeutic cues (NGF and diclofenac sodium) offers a promising strategy for the regeneration of severed peripheral nerves.

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Oleic acid promotes the expression of neural markers in differentiated human endometrial stem cells

Cited by 12 publications

References 32 publications

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

The Differential Metabolomes in Cumulus and Mural Granulosa Cells from Human Preovulatory Follicles

Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin

Gellan-Xanthan Hydrogel Conduits with Intraluminal Electrospun Nanofibers as Physical, Chemical and Therapeutic Cues for Peripheral Nerve Repair

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