Oleic acid promotes MMP-9 secretion and invasion in breast cancer cells

Soto-Guzman, Adriana; Navarro-Tito, Napoleón; Castro-Sánchez, Luis; Martinez-Orozco, Raul; Salazar, Eduardo Pérez

doi:10.1007/s10585-010-9340-1

Cited by 91 publications

(70 citation statements)

References 72 publications

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“…Studies indicated that mediumand long-chain free fatty acids are implicated in the activation of G protein-coupled receptors expressed in breast cancer cell lines (69)(70)(71). Some of these lipids are also known to be involved in the migration, proliferation, and invasion of breast cancer cells (i.e., oleic acid) or more generally in tumor processes (i.e., arachidonic acid) (69,(71)(72)(73)(74). About 85% of samples have a significant increase in ion abundance in the low-mass region (i.e., below m/z 700) in tumor samples, whereas most ions in the high-mass range (e.g., m/z 885.7) exist in both the tumor and normal specimen.…”

Section: Discussionmentioning

confidence: 99%

Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

Calligaris

Caragacianu

Liu

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

213

202

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Distinguishing tumor from normal glandular breast tissue is an important step in breast-conserving surgery. Because this distinction can be challenging in the operative setting, up to 40% of patients require an additional operation when traditional approaches are used.Here, we present a proof-of-concept study to determine the feasibility of using desorption electrospray ionization mass spectrometry imaging (DESI-MSI) for identifying and differentiating tumor from normal breast tissue. We show that tumor margins can be identified using the spatial distributions and varying intensities of different lipids. Several fatty acids, including oleic acid, were more abundant in the cancerous tissue than in normal tissues. The cancer margins delineated by the molecular images from DESI-MSI were consistent with those margins obtained from histological staining. Our findings prove the feasibility of classifying cancerous and normal breast tissues using ambient ionization MSI. The results suggest that an MSbased method could be developed for the rapid intraoperative detection of residual cancer tissue during breast-conserving surgery.B reast cancer is the most commonly diagnosed carcinoma in women in the United States and Western countries. Breast conservation surgery (BCS) has become the preferred treatment option for many women with early-stage breast cancer (1). BCS entails resection of the tumor, with a clean margin of normal tissue around it. Surgery is usually followed by radiation therapy. Results from seven large randomized prospective studies, with the largest two having over 20 y of follow-up, have shown equal survival when comparing BCS coupled with whole-breast radiation and mastectomy (2, 3).Normally, breast surgeons aim to remove a patient's tumor, along with a rim of normal tissue that is free of cancer. Preoperative mammography, ultrasonography, or MRI may be used by the surgeon to guide adequate resection (4-6). Despite numerous improvements in imaging and surgical technique, the need for reexcision to achieve complete tumor resection in the United States typically ranges from 20-40% (7-15), and has been reported as being as high as 60% (16). The importance of reexcision is underscored by numerous studies, which have shown that incomplete resection of tumor and positive margins are associated with increased locoregional recurrence compared with negative margins (12,(17)(18)(19)(20). Furthermore, the landmark meta-analysis performed by the Early Breast Cancer Trialists' Collaborative Group (18, 21) directly linked local recurrence to survival, placing great emphasis on the surgeon's role in minimizing local recurrence by obtaining adequate margins.Breast tumor reexcisions are accompanied by a number of undesirable problems: The completion of therapy is delayed, infection rates are increased, cost is increased, there can be a negative psychological impact on the patient, and there can be diminished aesthetic outcomes (22-24). The development of an intraoperative technique that allows the fast and accurate ident...

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Section: Discussionmentioning

confidence: 99%

Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

Calligaris

Caragacianu

Liu

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

213

202

View full text Add to dashboard Cite

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“…29 Additionally, the upregulation of MMP-9 (and MMP-2) has also been associated with obesity and may contribute to the increased invasiveness of cancers such as oesophagal adenocarcinoma. 43 The increased MMP levels in obesity may be a consequence of increased dietary fat consumption, including oleate, 44 or of increased levels of adipocytokines such as resistin. 45 In addition, the expression of these enzymes is also known to be potentiated under hypoxia conditions.…”

Section: Discussionmentioning

confidence: 99%

Trophoblast Invasion and Blood Vessel Remodeling Are Altered in a Rat Model of Lifelong Maternal Obesity

et al. 2014

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Maternal obesity is associated with an increased risk of a number of pregnancy complications, including fetal demise, which may be linked to impaired placental development as a result of altered trophoblast invasion and vessel remodeling. Therefore, we examined these parameters in pregnant rats fed a control (normal weight) or high fat (HF) diet (obese) at 2 critical times of rat placental development. Early trophoblast invasion was increased by approximately 2-fold in HF-fed dams with a concomitant increase in the expression of matrix metalloproteinase 9 protein, a mediator of tissue remodeling and invasion. Furthermore, we observed significantly higher levels of smooth muscle actin surrounding the placental spiral arteries of HF-fed dams, suggesting impaired spiral artery remodeling. Taken together, the results of this study suggest that altered placental development is an important contributor to the poor pregnancy outcomes and increased fetal demise in our model of lifelong maternal obesity.

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“…The negative regulation of the expression of β-catenin and the downstream factors of Wnt/β-catenin pathway by TOB1 inhibited β-catenin transcriptional activity [49] . In the 95-D/TOB1 and A549-siRNA TOB1 cells, varied MMP-2 and MMP-9 expression and activity is responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin, and proteoglycans [50][51][52] . Thus, suppression of β-catenin-regulated transcription, which induces the expression of proliferative and progressive genes such as cyclin D1 and MMPs via the PI3K/PTEN pathway, may at least mediate the anti-proliferative and anti-metastasis function of TOB1 in lung cancer cells [53,54] .…”

Section: Discussionmentioning

confidence: 99%

Suppression of human lung cancer cell proliferation and metastasis in vitro by the transducer of ErbB-2.1 (TOB1)

et al. 2011

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Aim: To investigate the effects of the transducer of ErbB-2.1 (TOB1) on the proliferation, migration and invasion of human lung cancer cells in vitro. Methods: Human lung cancer cell lines (95-D, A549, NCI-H1299, NCI-H1975, NCI-H661, NCI-H446, NCI-H1395, and Calu-3) and the normal human bronchial epithelial (HBE) cell line were tested. The expression levels of TOB1 in the cells were determined with Western blot and RT-PCR analyses. TOB1-overexpressing cell line 95-D/TOB1 was constructed using lipofectamine-induced TOB1 recombinant plasmid transfection and selective G418 cell culture. The A549 cells were transcend-transfected with TOB1-siRNA. MTT assay, flow cytometry and Western blot analysis were used to examine the effects of TOB1 on cancer cell proliferation and wound healing. Transwell invasive assay was performed to evaluate the effects of TOB1 on cancer cell migration and invasion. The activity of MMP2 and MMP9 was measured using gelatin zymography assay. Results: The expression levels of TOB1 in the 8 human lung cancer cell lines were significantly lower than that in HBE cells. TOB1 overexpression inhibited the proliferation of 95-D cells, whereas TOB1 knockdown with TOB1-siRNA promoted the growth of A549 cells. Decreased cell migration and invasion were detected in 95-D/TOB1 cells, and the suppression of TOB1 enhanced the metastasis in A549 cells. TOB1 overexpression not only increased the expression of the phosphatase and tensin homolog (PTEN), an important tumor suppressor, but also regulated the downstream effectors in the PI3K/PTEN signaling pathway, including Akt, ERK1/2, etc. In contrast, decreased expression of TOB1 oppositely regulated the expression of these factors. TOB1 also regulates the gelatinase activity of MMP2 and MMP9 in lung cancer cells. Conclusion:The results demonstrate that the PI3K/PTEN pathway, which is essential for carcinogenesis, angiogenesis, and metastasis, may be one of the possible signaling pathways for regulation of proliferation and metastasis of human lung cancer cells by TOB1 in vitro.

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Oleic acid promotes MMP-9 secretion and invasion in breast cancer cells

Cited by 91 publications

References 72 publications

Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

Application of desorption electrospray ionization mass spectrometry imaging in breast cancer margin analysis

Trophoblast Invasion and Blood Vessel Remodeling Are Altered in a Rat Model of Lifelong Maternal Obesity

Suppression of human lung cancer cell proliferation and metastasis in vitro by the transducer of ErbB-2.1 (TOB1)

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