Oleate accumulation, induced by silencing of microsomal omega-6 desaturase, declines with leaf expansion in transgenic tobacco

Yang, Mo; Xu, Yinong

doi:10.1016/j.jplph.2005.11.002

Cited by 12 publications

(8 citation statements)

References 20 publications

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“…Recently more mutant alleles with smaller lesions on FAD2-1 genes have been identified and were used to produce nontransgenic stable soybean lines with high oleic acid contents (Dierking and Bilyeu, 2009;Lee et al, 2009;Pham et al, 2010Pham et al, , 2011Bolon et al, 2011). (Stoutjesdijk et al, 2002), canola (Brassica napus L.) (Stoutjesdijk et al, 2000), cotton (Gossypium hirsutum L.) (Chapman et al, 2001;Liu et al, 2002;Sunilkumar et al, 2005), peanut (Arachis hypogaea L.) (Yin et al, 2007), and tobacco (Nicotiana tabacum L.) (Yang and Xu, 2007). Similar studies have been reported in other plants including Arabidopsis thaliana (L.) Heynh.…”

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confidence: 66%

“…Similar studies have been reported in other plants including Arabidopsis thaliana (L.) Heynh. (Stoutjesdijk et al, 2002), canola (Brassica napus L.) (Stoutjesdijk et al, 2000), cotton (Gossypium hirsutum L.) (Chapman et al, 2001;Liu et al, 2002;Sunilkumar et al, 2005), peanut (Arachis hypogaea L.) (Yin et al, 2007), and tobacco (Nicotiana tabacum L.) (Yang and Xu, 2007).…”

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confidence: 99%

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Molecular Characterization, Compositional Analysis, and Germination Evaluation of a High‐Oleic Soybean Generated by the Suppression ofFAD2‐1Expression

et al. 2014

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Soybean [Glycine max (L.) Merr.] oil is one of the most consumed and highest produced vegetable oils. However, the high percentage of polyunsaturated fatty acids in soybean oil limits its stability and shelf life. Here we report the generation and characterization of a high oleic acid soybean event (305423 soybean) that has an elevated content of monounsaturated oleic acid and reduced presence of polyunsaturated linoleic acid and linolenic acid. This transgenic event was generated by the insertion of a soybean ω‐6 desaturase (FAD2) gene fragment (gm‐fad2‐1), resulting in the suppression of endogenous FAD2‐1 expression, and a modified version of the soybean acetolactate synthase (ALS) gene (gm‐hra), used as a selectable marker. Molecular characterization revealed that 305423 soybean harbors four DNA inserts containing multiple copies of complete and partial gm‐fad2‐1 gene cassettes and a single copy of the intact gm‐hra gene cassette. However, these DNA inserts seemed to be inherited together without segregation. The suppression of endogenous FAD2‐1 led to an increase of oleic acid (18:1) from 211 to 765 g kg–1 along with concurrent reduction of both linoleic acid (18:2) from 525 to 36.2 g kg–1 and linolenic acid (18:3) from 93.5 to 53.9 g kg–1 out of total fatty acyl groups. The 305423 soybean was also subjected to nutrient composition analyses and phenotypic and agronomic performance evaluations and found to be comparable to the control soybean except for the expected traits.

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supporting

confidence: 66%

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confidence: 99%

Molecular Characterization, Compositional Analysis, and Germination Evaluation of a High‐Oleic Soybean Generated by the Suppression ofFAD2‐1Expression

et al. 2014

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“…To overcome the limitations of conventional breeding approaches, genetic engineering techniques have been successfully employed to modify fatty acid composition in a number of oilseed crops (Knutzon et al 1992;Shi et al 2001;Xiong et al 2003;Bu et al 2003;Li et al 2004;Dong et al 2004;Jadhav et al 2005;Nabloussi et al 2005;Yang and Xu 2007). The discovery of RNA interference in plants, mediated by sequence-specific degradation of dsRNA, has led to the development of highly efficient methods to silence plant genes (Stoutjesdijk et al 2000(Stoutjesdijk et al , 2002Liu et al 2002;Yang and Xu 2007;Takeno et al 2005;Husken et al 2005a,b;Liu et al 1996a,b). The level of unsaturated seed oil has been successfully modified through RNA interference technology and subsequently used to silence FAD2 in several plant species (Wesley et al 2001;Liu et al 2002;Stoutjesdijk et al 2002).…”

Section: Introductionmentioning

confidence: 99%

High-Oleic Peanut Oils Produced by HpRNA-Mediated Gene Silencing of Oleate Desaturase

et al. 2007

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The quality of peanut oil largely depends on the quantity of oleic (18:1) and linoleic acids (18:2). These two acids comprise more than 80% of the total fatty acids in peanuts. The oleate desaturase (FAD2) gene is important for maintaining high oleic acid content. A partial conservative sequence of the FAD2 gene from peanut was selected. The sense and antisense 260-bp fragments were amplified and subcloned into pFGC1008 binary expression vectors. A total of 21 transgenic plants were obtained via Agrobacterium-mediated transformation. The resulting down-regulation of the FAD2 gene resulted in a 70% increase in oleic acid content in the seeds of transformed plants compared with a 37.93% increase in untransformed plants. The results demonstrated that the target genes were likely suppressed by hpRNA interference, a pathway capable of achieving phenotypic changes. The silencing of FAD2 enabled the development of peanut oils having novel combinations of oleic acid content that can be used in high-value applications, making this approach a reliable technique for the genetic modification of seed quality and the potential for enhancement of other traits as well.

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“…Overexpression of FAD2 increased the germination rate of B. napus Based on variations in lipid contents during leaf expansion in FAD2-silenced tobacco, Yang and Xu (2007) proposed the putative role of FAD2 in plant development and selfdefense mechanisms. In our study, the overall up-regulation of FAD2 altered the physiological characteristics of FAD2 transgenic B. napus in the germination experiments.…”

Section: Resultsmentioning

confidence: 99%

“…Two cotton FAD2 isoform genes showed a periodical expression pattern that was lightand low temperature-dependent. Silencing of microsomal omega-6 desaturase was found to stop leaf expansion in transgenic tobacco (Yang and Xu 2007). It was assumed that FAD2 could promote plant growth via enhancement of fatty acid synthesis or interaction with other signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of FAD2 promotes seed germination and hypocotyl elongation in Brassica napus

Wang

Liu

et al. 2010

Plant Cell Tiss Organ Cult

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The enzyme fatty acid desaturase 2 (FAD2) transforms oleic acid (C18:1) to linoleic acid (C18:2) in plants and as such is involved in fatty acid synthesis. It is also involved in plant development and self-defense, such as seed germination, leaf expansion and cold resistance. We have cloned the full coding region of the Brassica napus FAD2 gene and ectopically expressed it in B. napus expressing low levels of FAD2. Overexpression of FAD2 under the control of the CaMV 35S promoter resulted in an up-regulated FAD2 mRNA level in B. napus as expected. Further analysis revealed that the FAD2 transgenic lines varied greatly in terms of their physiological characteristics, such as enhanced seed germination and increased hypocotyl length, compared to non-transgenic plants, suggesting that up-regulated FAD2 can promote seed germination and hypocotyl elongation in B. napus. Our results demonstrate the possible roles of FAD2 in plant development and also provide a platform for further analysis of fatty acid synthesis in plants.

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Oleate accumulation, induced by silencing of microsomal omega-6 desaturase, declines with leaf expansion in transgenic tobacco

Cited by 12 publications

References 20 publications

Molecular Characterization, Compositional Analysis, and Germination Evaluation of a High‐Oleic Soybean Generated by the Suppression ofFAD2‐1Expression

Molecular Characterization, Compositional Analysis, and Germination Evaluation of a High‐Oleic Soybean Generated by the Suppression ofFAD2‐1Expression

High-Oleic Peanut Oils Produced by HpRNA-Mediated Gene Silencing of Oleate Desaturase

Overexpression of FAD2 promotes seed germination and hypocotyl elongation in Brassica napus

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