Oleanolic Acid Controls Allergic and Inflammatory Responses in Experimental Allergic Conjunctivitis

Cordova, Claudia; Gutiérrez, Beatriz; Martínez‐García, Carmen; Martín, Rubén; Gallego‐Muñoz, Patricia; Hernández, Marita; Nieto, Marı́a Luisa

doi:10.1371/journal.pone.0091282

Cited by 36 publications

(26 citation statements)

References 41 publications

(34 reference statements)

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“…7). to have antiallergic properties [3] as well as being useful when uploaded onto nanoplatforms in anti-inflammatory applications [1]. In our study we showed that, in principle, only oleanolic acid at concentrations lower than 50 µM is not toxic for human corneal epithelial cells.…”

Section: Oa and Ua Impact On Production Of Metalloproteinases (Mmp-2 mentioning

confidence: 53%

“…These compounds exert many biological activities such as anti-inflammatory, anticancer, antioxidant, and antiallergic [1,2]. In view of a wide variety of activities, they were also considered as suitable in ophthalmology in order to protect the eye surface against adverse effects of particular inflammations and allergies [1,3]. Some of the factors acting adversely may include matrix metalloproteinases (MMPs) or high levels of nitric oxide (NO).…”

Section: Introductionmentioning

confidence: 99%

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The impact of oleanolic and ursolic acid on corneal epithelial cells in vitro

et al. 2017

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introduCtion. Oleanolic (OA) and ursolic (UA) acids belong to the triterpene group widely present in plants. These compounds are recognised to have anti-inflammatory properties and thus are considered to be used in therapies as well as in cosmetic, natural health, or diet products. aiM. The scientific hypothesis of our study was to show that OA and UA influence corneal epithelial cells cultured in vitro. Methods. Toxicity tests, based on MTT and Neutral Red (NR) uptake, measurement of nitric oxide (NOx) level, as well as analysis of metalloproteinases (MMP-2 and MMP-9) amount and activity were performed. resuLts. UA expressed significantly higher toxicity on cells than OA. At the lowest concentration applied (5 µM), UA limited cellular metabolism and viability on average by 22% as compared to untreated control, while 25 µM resulted in values lower than 10%. On the other hand, OA at the highest (100 µM) concentration limited cellular metabolism and viability by about 20%. NOx level significantly increased when OA and UA were applied at concentrations of 25 and 100 µM, respectively. OA and UA had a stronger impact on the level of MMP-2 than MMP-9. OA and UA reduced MMP-2 and MMP-9 in the whole range of concentrations. Tested triterpenoids had no significant impact on MMP activity. ConCLusions. OA and UA have a different impact on human corneal epithelial cells. UA is toxic for corneal epithelial cells, while OA exhibits milder activity, which may be useful for further analysis in ocular pharmacology.

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Section: Oa and Ua Impact On Production Of Metalloproteinases (Mmp-2 mentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

The impact of oleanolic and ursolic acid on corneal epithelial cells in vitro

et al. 2017

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“…This disease encompasses a diversity of pathological conditions and fundamental immunopathological mechanisms. Therefore, AC can be subdivided into seasonal and perennial allergic conjunctivitis . Previous data suggest that 15%–40% of the population world‐wide are affected by AC .…”

Section: Role Of Il‐1β In Allergic Conjunctivitis (Ac)mentioning

confidence: 99%

NLRP3 inflammasome: A likely target for the treatment of allergic diseases

Xiao

2018

Clin Experimental Allergy

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Allergic diseases, such as asthma, rhinitis, dermatitis, conjunctivitis, and anaphylaxis, have recently become a global public health concern. According to previous studies, the NLRP3 inflammasome is a multi-protein complex known to be associated with many inflammatory conditions. In response to allergens or allergen/damage-associated molecular signals, NLRP3 changes its conformation to allow the assembly of the NLRP3 inflammasome complex and activates caspase-1, which is an evolutionarily conserved enzyme that proteolytically cleaves other proteins, such as the precursors of the inflammatory cytokines IL-1β and IL-18. Subsequently, active caspase-1 cleaves pro-IL-1 and pro-IL-18. Recently, accumulating human and mouse experimental evidence has demonstrated that the NLRP3 inflammasome, IL-1β, and IL-18 are critically involved in the development of allergic diseases. Furthermore, the application of specific NLRP3 inflammasome inhibitors has been demonstrated in animal models. Therefore, these inhibitors may represent potential therapeutic methods for the management of clinical allergic disorders. This review summarizes findings related to the NLRP3 inflammasome and its related factors and concludes that specific NLRP3 inflammasome inhibitors may be potential therapeutic agents for allergic diseases.

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“…The current agents used for AC treatments include antihistamines, mast cell stabilizers, nonsteroidal anti-inflammatory drugs, and topical corticosteroids. 23,24 These agents are known to cause side effects after long-term use. As such, further research is mandatory for development of better treatment regimens.…”

mentioning

confidence: 99%

A Murine Model of Acute Allergic Conjunctivitis Induced by Continuous Exposure to Particulate Matter 2.5

Tang

Chang

Chiang

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Several pieces of epidemiologic evidence have indicated PM2.5 (particulate matter with a diameter of 2.5 lm or less) as a causing factor of allergic conjunctivitis, but without experimental elucidation of mechanism. In the present study, PM2.5 in solution was directly applied to the mouse ocular surface to elucidate whether PM2.5 might cause allergic conjunctivitis, and its underlying mechanisms were analyzed. METHODS. ICR mice were challenged for 18 consecutive days with eye drops containing PM2.5 at 3.2, 6.4, and 12.8 mg/mL in 0.9% NaCl saline, along with the controls prepared in parallel without PM2.5 and another control group treated with both PM2.5 at 12.8 mg/mL and artificial tears. On day 19, the whole eyes and meibomian glands were harvested for histopathological analyses and assessment of clinical scoring, tear volume, tear breakup time, and tear ferning. Furthermore, goblet cells by periodic acid Schiff stain and infiltrated eosinophils by Giemsa stain were quantified and compared among study groups. RESULTS. Clinical scoring showed more eyelid edema, tearing, and scratching behaviors, with longer tear breakup time under the influence of increased PM2.5 concentrations. Tear ferning assay showed less tear crystal formation and decreased crystal branches after exposure to PM2.5. In addition, higher goblet cell density in the upper palpebral conjunctiva and extensive eosinophil infiltration in the entire conjunctiva and in the meibomian glands were induced by PM2.5. CONCLUSIONS. These results demonstrate that PM2.5 can induce symptoms similar to clinical allergic conjunctivitis and that the murine acute allergic conjunctivitis model can be induced by direct exposure to PM2.5.

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Oleanolic Acid Controls Allergic and Inflammatory Responses in Experimental Allergic Conjunctivitis

Cited by 36 publications

References 41 publications

The impact of oleanolic and ursolic acid on corneal epithelial cells in vitro

The impact of oleanolic and ursolic acid on corneal epithelial cells in vitro

NLRP3 inflammasome: A likely target for the treatment of allergic diseases

A Murine Model of Acute Allergic Conjunctivitis Induced by Continuous Exposure to Particulate Matter 2.5

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