“…The 1 H NMR spectrum of 1 showed a broad singlet at δH 5.30 for an olefinic proton, overlapped signals from δH 3.20 to 4.38 for oxygenated methine/methylene protons, seven singlets at δH 1.40, 1.09, 1.05, 1.00, 0.97, 0.93, and 0.88 for methyl protons, and the others in the region from δH 2.28 to 0.82. Among a total of 41 carbons present in this molecule, 40 resonances were observed in the 13 C NMR spectrum of 1, including 30 peaks for typical oleanane-type triterpenoids [14][15][16][17][18] with two olefinic carbons at δC 144.6 and 124.2 and four oxygenated carbons at δC 91.1, 78.8, 70.4, and 65.8, and ten peaks for β-glucuronic acid (δC 106.9, 75.6, 78.1, 73.8, and 76.8) and β-xylopyranose (δC 102.8, 74.5, 77.0, 71.2 and 66.5). These 1 H and 13 C NMR data implied that 1 is an oleanane-type triterpenoidal glycoside with two sugar moieties, and its core structure including the location of a double bond and four oxygenated carbons was established through analysis of the Distortionless Enhancement by Polarization Transfer (DEPT), correlation spectroscopy (COSY), Heteronuclear Single Quantum Correlation (HSQC), and Heteronuclear Multiple Bond Correlation (HMBC) spectra (Figure 2, Supplementary materials).…”