Okanin from Coreopsis tinctoria Nutt. alleviates cognitive impairment in bilateral common carotid artery occlusion mice by regulating the miR-7/NLRP3 axis in microglia

Abstract

Cognitive impairment is the main clinical feature following stroke, and microglia-mediated inflammatory response is a major contributor to it. Coreopsis tinctoria Nutt., an edible chrysanthemum, is commonly used as a...

Hyperforin ameliorates neuroinflammation and white matter lesions by regulating microglial VEGFR2/SRC pathway in vascular cognitive impairment mice

“…Thereafter, 10% fetal bovine serum and 1% Penicillin–Streptomycin (PS) were added to a DMEM medium to culture BV2 cells. Oxygen–glucose deprivation/reperfusion (OGD/R) was employed to simulate acute cerebral ischemia‐hypoxia reperfusion and induce microglial activation 45,46 . In a specialized hypoxic incubator, the cells are subjected to a glucose‐free medium for 2 h to induce OGD/R.…”

“…Oxygen-glucose deprivation/reperfusion (OGD/R) was employed to simulate acute cerebral ischemia-hypoxia reperfusion and induce microglial activation. 45,46 In a specialized hypoxic incubator, the cells are subjected to a glucose-free medium for 2 h to induce OGD/R. The hypoxic incubator was filled with a gas mixture with 94% N 2 , 5% CO 2 , and 1% O 2 .…”