Okajimas Folia Anat. Jpn., 58(1): 55-68, May 1981 New Method of Embedding with GMA, Quetol 523 and Methyl Methacrylate for Light and Electron Microscopic Observation of Semi-thin Sections

Abstract

The resolution of the electron microscope was so high that the cytoplasmic components were readily identified in the cytoplasm. Osmium tetroxide vapor staining gave better contrast in the images of the specimens.

Observation on the cycle of the seminiferous epithelium in the Japanese macaque (Macaca fuscata) using semithin sections

“…After drying, they were stained with a modified PAS reaction and counterstained with hematoxylin (NAGATO et al, 1984). For the observation of cytoplasmic structure of the germ cells, tissue was also fixed by immersion in a mixture of formaldehyde and glutaraldehyde (NAGATO et al, 1981) and stained with hematoxylin and eosin. After staining, the specimens were dried and covered with a coverslip using an appropriate mounting resin.…”

“…In recent years, several embedding methods employing a water-miscible methacrylate have been introduced in the field of light microscopy in place of ordinary paraffin embedding methods (BENNETT et al, 1976;KUSHIDA et al, 1981;WYNFORD-THOMAS et al, 1981;CHAPPERD et al, 1983;RUDDELL, 1983;COLE, 1984;KUSHIDA et al, 1985;MYHRE & DEPAOLI, 1985;HOTT & MARIE, 1987;LIU, 1987). These advanced techniques of histology have been developed to improve the preservation of cellular structures and to increase the resolution of light microscopic images by means of their thickness.…”

“…These advanced techniques of histology have been developed to improve the preservation of cellular structures and to increase the resolution of light microscopic images by means of their thickness. Among them, Quetol 523M and its related methods have added much to our understanding of the morphological and histochemical findings in various structures when using both light and electron microscopy (SUZUKI 8r NAGATO, 1980;KUSHIDA et al, 1981KUSHIDA et al, , 1985NAGATO et al, 1981NAGATO et al, , 1984NAGATO et al, , 1989NAGATO et al, , 1991hJ1MA et al, 1992). Staining affinity to the PAS reaction was also improved during the development of the embedding method (NAGATO et al, 1984).…”

Use of Semithin Sections Embedded in a Water-Miscible Methacrylate for Light Microscopy of Central Nerve Tissues

“…A watermiscible methacrylate, glycol methacrylate (CMA), was first developed as an embedding medium for light microscopy. Several embedding methods using GMA have been described for light microscopy of soft and hard tissues (Ruddell, 1961;Cpole and Sykes, 1974;Kushida et al, 1974Kushida et al, , 1981Bennett et al, 1976;Myhre and Depaoli, 1985;Hott and Marie, 1987;Liu, 1987). Ruddell (1967) first used GMA for biological specimens.…”

“…This embedding method allowed application of enzyme and immunohistochemical techniques (Nagato et al, 1979(Nagato et al, , 1984Suzuki and Nagato, 1980). Adaptation for electron microscopy was also possible being semithin sections 0.2-0.3 pm thick according to the method of Kushida (1977) and Kushida et al (1981).…”