OK-432 Induces Production of Neutrophil Chemotactic Factors in Malignant Pleural Effusion.

Abstract

Weinvestigated the changes in cellular components and neutrophil chemotactic factors in pleural fluid from 19 lung cancer patients who received intrapleural injection of OK-432 to treat malignant pleurisy. Not only neutrophil chemotactic activity (NCA) but also neutrophil count and percentage were increased significantly at 6 hours after OK-432injection. The neutrophil count was significantly correlated with NCAlevel. The levels of C5a and IL-8 in pleural fluid were increased significantly after OK-432 injection. The increased IL-8 level was associated with a increase of both NCAand neutrophil count. OK-432 treatment also induced a marked increase of IL-lfi and IL-6 in pleural fluid. Thus, intrapleural injection of OK-432 induced production of neutrophil chemotactic factors (IL-8 and C5a) and cytokines (IL-lfi and IL-6), which eventually attracted neutrophils into the pleural space. These observations suggest that neutrophil migration mediated by these factors and cytokines may contribute to the sclerosing effects of OK-432 treatment. (Internal Medicine 34: 352-356, 1995)

Pulmonary perspective: immunology in diagnosis and treatment of lung cancer

“…It is crucial to know whether these regressions of MPE are mediated by specific (CTL) or aspecific (NK) antitumoral effector cells, or only by the sclerosing effect secondary to the release of inflammatory cytokines, such IL-8, by the mesothelial cells. Thus, it was clearly demonstrated that the control of MPE by instillation of OK-432 tetracycline, Corynebacterium parvum was not mediated by immune cells, but by the inflammatory response induced by the release from mesothelial cells of C5a, IL-8, IL-6 or TNF, leading to pleural sclerosis [177,179,180].…”

Effect of Intraperitoneal Neutrophils Induced by OK432 on Malignant Ascites

“…40 Although the chemotactic activity and vascular permeability-increasing activity of C5a are thought to increase the adhesion of neutrophils to the peritoneal vascular network, 41 we focused our attention on milky spots. The milky spots of the greater omentum are important tissue for intraperitoneal immunity, involved in the migration of neutrophils, macrophages, and lymphocytes.…”

New agents for pleurodesis

“…In rats, OK-432 (0.3 KE kg −1 ) is less effective than talc (400 mg kg −1 ) in inducing pleurodesis at 30 days [33]. In Japan, it is regarded by some as the sclerosing agent of choice [34]. Response as high as 75 % has been achieved.…”