Oilseed rape—a new allergen?

Fell, P. J.; Soulsby, S.; Blight, Margaret M.; Brostoff, Jonathan

doi:10.1111/j.1365-2222.1992.tb00154.x

Cited by 34 publications

(38 citation statements)

References 15 publications

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“…Therefore, allergy to Brassica or Lilium pollen is uncommon in the population. However, epidemiological studies showed that the incidence of Brassica pollen allergy is much higher in individuals occupationally exposed to Brassica pollen (54).…”

Section: Iontophoresis Of Bet V 4 Into Growing Lilymentioning

confidence: 99%

Immunological and Biological Properties of Bet v 4, a Novel Birch Pollen Allergen with Two EF-hand Calcium-binding Domains

Engel

Richter

Obermeyer

et al. 1997

Journal of Biological Chemistry

123

126

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Section: Iontophoresis Of Bet V 4 Into Growing Lilymentioning

confidence: 99%

Immunological and Biological Properties of Bet v 4, a Novel Birch Pollen Allergen with Two EF-hand Calcium-binding Domains

Engel

Richter

Obermeyer

et al. 1997

Journal of Biological Chemistry

123

126

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“…These molecules were identified by their ability to bind to human IgE. As oilseed rape pollen allergy is not a well-defined entity, we chose to test human sera from birch- and/or orchard grass-allergic patients because the pollinic season of oilseed rape and birch overlap in spring [2]. The other patients, who were allergic to mustard, were chosen because mustard belongs to the Brassicaceae, as does oilseed rape.…”

Section: Discussionmentioning

confidence: 99%

“…Since 1954, several reports have examined the question of the potential role of oilseed rape (Brassica napus) in pollen allergy [1, 2, 3, 4, 5, 6, 7]. Some of them concluded that type I hypersensitivity to rape pollen is limited to atopic patients with a history of pollen allergy [2, 3].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of High-Molecular-Mass Allergens in Oilseed Rape Pollen

Chardin

Mayer

Sénéchal

et al. 2001

Int Arch Allergy Immunol

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Background:Oilseed rape pollen allergies have been previously described as the result of cross-sensitization with various pollens. Recently, several proteins have been identified as oilseed rape allergens. The aim of the present work was the characterization of oilseed rape pollen allergens by two-dimensional (2-D) gel analysis and amino acid microsequencing. Methods: Water extractable proteins from oilseed rape pollen were separated by isoelectrofocusing and then transferred onto a nitrocellulose sheet. Twenty-one human sera from pollen- or mustard-allergic individuals were screened for their reactivity to oilseed rape proteins. Eleven sera possessed IgE which recognized oilseed rape pollen proteins and one serum was selected for further 2-D characterization and amino acid microsequencing of the allergens. Results: The results showed that three molecules from oilseed rape pollen were identified as oilseed rape allergens which have not yet been described. These three proteins were molecules of 70 kD with a pI >8, 40 kD with a pI around 10 and 80 kD with a pI around 5. These proteins displayed identities with the berberine bridge protein, a receptor-like protein kinase and the cobalamin-independent methionine synthetase from Arabidopsis thaliana, respectively. The genes encoding the putative Arabidopsis molecules are located on chromosome 1 (berberine bridge protein) and chromosomes 3 and 4 (receptor-like protein kinases). Conclusion: These results show that certain high-molecular-mass proteins from oilseed rape pollen are allergens.

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“…While complaints attributed to OSR cultivation have been considered to be caused by irritant rape volatiles, such as terpenes, which are produced in high quantities by the plant for insect attraction or natural pathogen defense [3, 4], it has been known for nearly 50 years that OSR pollen may sensitize and provoke allergic symptoms [5]. Recent studies revealed conflicting results regarding the prevalence of OSR allergy but consistently proved the possibility of allergic sensitization to its pollen [1, 2, 6, 7]. The cultivation of the closely related species B. rapa (= campestris, turnip rape) has been recognized for years as a cause of allergic disease in India [8], and sensitization was also described from Japan [9].…”

Section: Introductionmentioning

confidence: 99%

Identification of Allergens in Oilseed Rape (Brassica napus) Pollen

Focke

Hemmer²,

Hayek³

et al. 1998

Int Arch Allergy Immunol

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Background: Pollen from oilseed rape (OSR), Brassica napus, an increasingly cultivated oilplant from the Brassicaceae, has been recognized as a potential cause of allergic sensitization. Allergens have been hardly investigated. Methods: We characterized IgE binding proteins in OSR pollen by immunoblot, immunoblot inhibition and specific monoclonal antibodies using sera from 89 patients sensitized to OSR. Results: Two low–molecular–weight allergens of 6/8 kD and 14 kD as well as a high molecular–weight cluster (27–69 kD) comprising six cross–reactive peptides could be identified. The three allergens were recognized by 50, 34 and 80% of patients, respectively. Immunoblot IgE binding to OSR could be totally inhibited by rye pollen and moderately by birch pollen (6/8 and 14 kD) while mugwort had little effect. An anti–profilin–specific monoclonal antibody bound specifically to a 14–kD protein in OSR. Binding to the 6/8–kD rape allergen could be effectively inhibited by rAln g 2, a calcium–binding protein from alder. Periodate treatment led to a significant reduction in IgE binding to the 27 to 69–kD OSR allergens indicating that carbohydrate determinants are involved in IgE binding. OSR proteins were capable to quench IgE binding to timothy grass pollen proteins of ≥60 kD suggesting that grass pollen group 4 allergens cross–react with the 27 to 69–kD cluster in OSR. Conclusions: The data demonstrate that OSR pollen is allergenic and indicate that the identified allergens represent cross–reacting homologues of well–known pollen allergens, i.e. calcium–binding proteins, profilins, and high–molecular–weight glycoproteins. Via cross–reactivity, exposure to OSR pollen may be a prolonging and aggravating factor in underlying birch and grass pollen allergy.

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Oilseed rape—a new allergen?

Cited by 34 publications

References 15 publications

Immunological and Biological Properties of Bet v 4, a Novel Birch Pollen Allergen with Two EF-hand Calcium-binding Domains

Immunological and Biological Properties of Bet v 4, a Novel Birch Pollen Allergen with Two EF-hand Calcium-binding Domains

Characterization of High-Molecular-Mass Allergens in Oilseed Rape Pollen

Identification of Allergens in Oilseed Rape (Brassica napus) Pollen

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