Oil rich in carotenoids instead of vitamins C and E as a better option to reduce doxorubicin-induced damage to normal cells of Ehrlich tumor-bearing mice: hematological, toxicological and histopathological evaluations

Miranda-Vilela, Ana Luisa; Grisólia, César Koppe; Longo, João Paulo Figueiró; Peixoto, Raphael Cândido Apolinário; Almeida, Marcos Célio de; Barbosa, Lilian Carla Pereira; Roll, Mariana Matos; Portilho, Flávia Arruda; Estevanato, Luciana Landim Carneiro; Bocca, Anamélia Lorenzetti; Báo, Sônia Nair; Lacava, Z.G.M.

doi:10.1016/j.jnutbio.2014.06.005

Cited by 40 publications

(38 citation statements)

References 51 publications

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“…Commonly, C. brasiliense fruits are used to treat several diseases, including tumors, several respiratory diseases, and ophthalmic problems (Miranda-Vilela et al, 2014;MirandaVilela et al, 2008), while fruits of C. coriaceum are popularly used to treat many types of afflictions, such as wound lesions, gastric and inflammatory diseases, respiratory tract infections, muscle pain, and chronic arthritis (Costa et al, 2011;Da Silva Quirino et al, 2009). As stated earlier, most of these effects are attributed to the oil that is extracted from the pulp of the Caryocar spp.…”

Section: Biological Effects Of Caryocar Fruit Consumptionmentioning

confidence: 99%

“…Evidence suggests that cancer cells are under increased oxidative stress compared with normal cells, and this is associated with oncogene-induced transformation, increased metabolic activity, mitochondrial malfunction, and increased generation of reactive oxygen species (ROS) (Miranda-Vilela et al, 2014). Many cancer-chemopreventive agents possess antioxidant potential, and biological antioxidants contain bioactive phytochemicals that may play a vital role in protecting cells from oxidative stress (Miranda-Vilela et al, 2011).…”

Section: Anticancer Activitymentioning

confidence: 99%

“…In this way, both pulp and almond are routinely used for therapeutic purposes by the regional population to treat e.g. tumors, respiratory diseases, wound lesions, gastric and inflammatory diseases, muscle pain, and chronic arthritis (Miranda-Vilela et al, 2014;Costa et al, 2011;Da Silva Quirino et al, 2009;MirandaVilela et al, 2008). Pequi presents some beneficial biological properties, such as wound-healing and anti-inflammatory activities, antimicrobial activity, and protection against genomic and oxidative damage, among others (Colombo et al, 2015;Costa et al, 2011;De Oliveira et al, 2010;Da Silva Quirino et al, 2009;Passos et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Bioactive compounds and functional potential of pequi (Caryocar spp.), a native Brazilian fruit: a review

et al. 2018

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SUMMARY:Pequi is an indigenous word that means "thorny covering" and is used to describe fruits from the Caryocar spp. These fruits are widely consumed as food and used in traditional medicine by Brazilians in the savannah (Cerrado biome) and the Amazon region. The fruit is rich in lipids, mainly oleic acid, and other bioactive substances including carotenoids, phenolics, and tocopherols. The oil extracted from the pulp or "almond" (seed) has a high local socioeconomic impact and is associated with nutritional and therapeutic benefits. A wide array of health benefits such as antioxidant, anti-inflammatory, antitumor, and antimicrobial effects, improved cardiac function, as well as an increased lymphocyte-dependent immunity have been attributed to the pequi fruit, especially its pulp. This review provides a comprehensive overview on the edible parts of pequi fruits (pulp and almond), more specifically the oil produced from these parts, as a source of functional compounds with biological activity. Moreover, it considers the differences among the three more commercially-important species from the genus Caryocar. KEYWORDS: Bioactive compounds; Caryocaraceae; Fruit; Health benefits; Oleic acid RESUMEN:Compuestos bioactivos y funcionalidad potencial del pequi (Caryocar spp.), fruta nativa brasileña. Revisión. Pequi es una palabra indígena que significa "piel espinosa" y es utilizada para describir los frutos de Caryocar spp. Estos frutos son ampliamente consumidos como alimentos y son utilizados en la medicina popular por los brasileños ubicados en el Savannah (bioma Cerrado) y en la región amazónica. La fruta es rica en grasas, ácido oleico y otros bioactivos, incluyendo carotenoides, fenoles y tocoferoles. El aceite procedente de la pulpa o de la almendra (semilla) tiene un importante impacto socioeconómico local y está asociado con beneficios nutricionales y terapéuticos. Una amplia gama de beneficios para la salud tales como antioxidante, antiinflamatorio, antitumoral, antimicrobiano, mejora de la función cardíaca, así como el aumento de la inmunidad linfocitaria han sido atribuidas a la fruta, especialmente a su pulpa. Esta revisión proporciona una descripción exhaustiva sobre las partes comestibles de la fruta del pequi (pulpa y almendra), más específicamente del aceite producido a partir de estas partes, como una fuente de compuestos funcionales con actividad biológica. Además se consideran las diferencias encontradas entre las tres especies comerciales más importantes del género Caryocar.

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Section: Biological Effects Of Caryocar Fruit Consumptionmentioning

confidence: 99%

Section: Anticancer Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bioactive compounds and functional potential of pequi (Caryocar spp.), a native Brazilian fruit: a review

et al. 2018

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“…Miranda-Vilela et al (2014) evaluated the effect of a carotenoidrich oil (pequi oil), instead of vitamins C and E, on the reduction of chemotherapy adverse effects to normal cells in Ehrlich solid tumor-bearing mice. The authors observed that the treatments with pequi oil provided before tumor inoculation or in continuous and concurrent administration with doxorubicin were more effective in containing tumor growth, in addition to increasing immunity and reducing the adverse side effects associated with oxidative damage to normal cells.…”

Section: Oil Characterizationmentioning

confidence: 99%

Chemical composition and oxidative stability of jussara (Euterpe edulis M.) oil extracted by cold and hot mechanical pressing

et al. 2018

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SUMMARY:The aim of this work was to evaluate the effect of mechanical pressing on jussara oil yield, oxidative stability and carotenoid profile with or without heat application. Firstly, jussara pulp was centrifuged for juice extraction, and the resulting cake was dried until reaching 10% moisture content. Then, oil extraction was performed in an expeller press at 25 ºC (cold pressing) and at 50 ºC (hot pressing). The process performance was evaluated by the oil yield, and the crude jussara oil was characterized for fatty acid composition, acid value, carotenoid profile and oxidative stability. Jussara oil contained 74% unsaturated fatty acids, mainly oleic and linoleic acids (48% and 24%, respectively). The oil yield was almost twice as high for the hot process as compared to the cold one. Additionally, hot pressing resulted in 25% higher total carotenoid content as compared to cold pressing, with β-carotene as the most abundant one. Hot and cold pressing showed no difference in oil oxidative stability and fatty acid composition. KEYWORDS:Carotenoids; Euterpe edulis; Extraction; Fatty acid profile; Oxidative stability RESUMEN: Composición química y estabilidad oxidativa del aceite de jussara (Euterpe edulis M.) extraído mediante prensado mecánico en frío y en caliente. El objetivo de este estudio fue evaluar el efecto del calor sobre el rendimiento del aceite, la estabilidad oxidativa y el perfil de carotenoides del aceite de Jussara extraído por prensado mecánico. En primer lugar, se centrifugó la pulpa de jussara para la extracción del zumo, y la torta resultante se secó para alcanzar el contenido de humedad de 10%. Posteriormente, la extracción del aceite se realizó en una prensa a 25 °C (prensado en frío) y a 50 °C (prensado en caliente). El desarrollo del proceso fue evaluado mediante el rendimiento de aceite y en bruto y éste se caracterizó mediante la composición en ácidos grasos, índice de acidez, perfil de carotenoides y la estabilidad oxidativa. El aceite de jussara presentó un 74% de ácidos grasos insaturados, principalmente ácidos oleico y linoleico (48% y 24%, respectivamente). El rendimiento del aceite para el proceso en caliente fue casi el doble, en comparación con el proceso en frío. Además, el prensado en caliente mostró un contenido total de carotenoides 25% mayor en comparación con el prensado en frío, siendo el β-caroteno, el carotenoide más abundante. Las prensas en calientes y en frío no mostraron diferencias en la estabilidad oxidativa del aceite ni en la composición de ácidos grasos.

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“…For this, the sample was dehydrated in a low vacuum environment and stored as a powder without atmospheric contact; using a Zetasizer (Zetasizer Nano-ZS90 Malvern Instruments Limited, Malvern, UK) we determined the hydrodynamic radius, the polydispersion index and its surface charge by dynamic light scattering (DLS) with the sample diluted in ultra-pure deionized water (1: 100), and the measurements were performed in triplicate at 25°C, with a fixed detection angle of 173°C; the physical mean diameter and its dispersion was determined by transmission electron microscopy (TEM) performed on a JEOL 1011 (JEOL USA, Inc.) with sample diluted in ultra-pure deionized water (1:500), deposited in a 300 mesh copper TEM grid supported by films of Formvar, dried at room temperature for 24 hours and then counterstained for 45 minutes with 1% Osmium (OsO 4 ) vapor. The average diameter of the sample evaluated by MET implantation [15,32,35,36]. Following 48 hours after xenograft, all mice had clinical tumor and received the following treatments: (a) filtered water and non-tumor-bearing mice (negative control); (b) xenograft and no treatment (tumor control); (c) intratumoral injection of NPCit (40 µL) containing 18 × 10 18 particles/mL and exposure for 13 minutes to CMagMHG; (d) intratumoral injection of NPCit and exposure for 13 minutes to Magnetherm.…”

Section: Magnetic Fluidsmentioning

confidence: 99%

Comparative Efficacy of a Biocompatible Citrate-Functionalized Magnetic Fluid Mediating Radiofrequency Hyperthermia and Magnetohyperthermia to Treat Ectopic Ehrlich-Solid-Tumor-Bearing Elderly Mice

Neves¹,

Sousa²,

Vilela³

et al. 2017

J Cancer Sci Ther

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Objective: We aimed to evaluate the efficacy of a biocompatible citrate-functionalized magnetic fluid (NPCit) sample in mediating hyperthermia, using two types of alternated (AC) magnetic field equipment operating in two different ways: a portable apparatus, generating an electromagnetic field in MHz range (radiofrequency range; radiofrequency hyperthermia-RFHT) developed by our research group (CMagMHG), and a commercial one working in kHz range (magnetohyperthermia-MHT) (Magnetherm, NanoTherics Ltd, Newcastle, United Kingdom), to treat ectopic Ehrlich-solid-tumor-bearing elderly mice. Methods:Females intraperitoneally anesthetized with ketamine (80 mg/kg) and xylazine (10 mg/Kg) were subcutaneously inoculated with Ehrlich ascitic tumor cell suspension (1.03 × 10 6 viable cells) in the upper head region for solid form implantation. After 48 hours, mice received one of four treatments: (a) filtered water and no tumor implantation (negative control); (b) tumor inoculation and no treatment (tumor control); (c) intratumoral injection of NPCit (40 µL) containing 18 × 10 18 particles/mL and 13 minutes' exposure to CMagMHG, operating at 1MHz, 40 Oe field amplitude; (d) intratumoral injection of NPCit and 13 minutes' exposure to Magnetherm, assembled with 17-turn coil, capacitive box B22, operating under 330 kHz, 4.9 A, 25 V and maximum field strength 170 Oe. Treatments occurred once daily for three consecutive days. Tumor histopathology and semi-quantitative analysis of necrosis area served to assess tumor aggressiveness and regression. Possible acute side effects were assessed by animals' body and spleen weight and hemogram.Results: NPcit showed adequate biocompatibility and was effective in mediating RFHT and MHT, which promoted significant increases in necrosis area using both equipment types. Conclusion:Our findings evidence the potential use of NPcit mediating hyperthermia for future use as an adjuvant in breast cancer therapy.

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Oil rich in carotenoids instead of vitamins C and E as a better option to reduce doxorubicin-induced damage to normal cells of Ehrlich tumor-bearing mice: hematological, toxicological and histopathological evaluations

Cited by 40 publications

References 51 publications

Bioactive compounds and functional potential of pequi (<em>Caryocar</em> spp.), a native Brazilian fruit: a review

Bioactive compounds and functional potential of pequi (<em>Caryocar</em> spp.), a native Brazilian fruit: a review

Chemical composition and oxidative stability of jussara (<em>Euterpe edulis</em> M.) oil extracted by cold and hot mechanical pressing

Comparative Efficacy of a Biocompatible Citrate-Functionalized Magnetic Fluid Mediating Radiofrequency Hyperthermia and Magnetohyperthermia to Treat Ectopic Ehrlich-Solid-Tumor-Bearing Elderly Mice

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