Oil Red O based method for exosome labelling and detection

Bharati, Shikha; Anjaly, Km; Thoidingjam, Shivani; Tiku, Ashu Bhan

doi:10.1016/j.bbrc.2022.04.087

Cited by 11 publications

(5 citation statements)

References 15 publications

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“…Our frog SDD-like deposits did not label strongly with Oil Red O or BODIPY (Fig. 2), indicating a lack of neutral lipids such as cholesterol esters and triacylglycerol (Bharati et al, 2022;Elle et al, 2010;Ramírez-Zacarías et al, 1992) and mimicking Oil Red O labeling results in human SDD. Brightfield and red autofluorescence imaging also revealed that the RPE in prom1null animals was fragmented, extended much further into the outer segment layer, and infiltrated into the SDD-like deposits when compared to wildtype animals (Fig.…”

Section: Resultsmentioning

confidence: 81%

Prominin-1 null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy, and RPE atrophy

Carr,

Skitsko,

Song

et al. 2024

Preprint

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Mutations in thePROMININ-1(PROM1)gene are associated with inherited, non-syndromic vision loss. Here, we used CRISPR/Cas9 to induce truncatingprom1-null mutations inXenopus laevisto create a disease model. We then tracked progression of retinal degeneration in these animals from the ages of 6 weeks to 3 years old. We found that retinal degeneration caused byprom1-null is age-dependent and likely involves death or damage to the retinal pigment epithelium (RPE) that precedes photoreceptor degeneration. Asprom1-null frogs age, they develop large cellular debris deposits in the subretinal space and outer segment layer which resemble subretinal drusenoid deposits (SDD) in their location, histology, and representation in color fundus photography and optical coherence tomography (OCT). In older frogs, these SDD-like deposits accumulate in size and number, and they are present before retinal degeneration occurs. Evidence for an RPE origin of these deposits includes infiltration of pigment granules into the deposits, thinning of RPE as measured by OCT, and RPE disorganization as measured by histology and OCT. The appearance and accumulation of SDD-like deposits and RPE thinning and disorganization in our animal model suggests an underlying disease mechanism forprom1-null mediated blindness of death and dysfunction of the RPE preceding photoreceptor degeneration, instead of direct effects upon photoreceptor outer segment morphogenesis, as was previously hypothesized.

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Section: Resultsmentioning

confidence: 81%

Prominin-1 null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy, and RPE atrophy

Carr,

Skitsko,

Song

et al. 2024

Preprint

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“…4C). Furthermore, Oil Red O staining, a fat-soluble dye to stain neutral lipids and cholesteryl esters ( 27, 28 ), revealed a diminished presence of intracellular lipid droplets, indicating lower lipid reserves (Fig. 4C).…”

Section: Resultsmentioning

confidence: 99%

ATP Citrate Lyase Drives Vascular Remodeling Diseases Development Through Metabolic-Epigenetic Reprograming

Grobs,

Romanet,

Lemay

et al. 2024

Preprint

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Our study explores the previously uncharted role of ATP-citrate lyase (ACLY) in vascular remodeling within the pulmonary and coronary arteries, providing novel insights into the pathogenesis of pulmonary hypertension and coronary artery diseases. ACLY, involved in de novo lipid synthesis and histone acetylation, has emerged as a key regulator in sustaining vascular smooth muscle cell (VSMC) proliferation and survival.Utilizing human coronary and pulmonary artery tissues, our findings reveal an upregulation of ACLY expression during vascular remodeling processes. Inhibition of ACLY, achieved through pharmacological and molecular interventions in humans primary cultured VSMCs, leads to decreased proliferation, migration, and resistance to apoptosis. Mechanistically, these effects are associated with diminished glycolysis, lipid synthesis, GCN5-dependent histone acetylation, and FOXM1 activation.In vivo experiments, combining pharmacological and VSMC-specific ACLY knockout mice, ACLY inhibition demonstrates its efficacy in mitigating coronary artery remodeling and reducing pulmonary hypertension. Notably, initiating ACLY inhibition post-disease onset reverses pathological conditions, positioning ACLY as a promising therapeutic target.Human ex vivo tissue culture further supports our findings, showing reduced vascular remodeling in cultured human coronary artery rings and a reversal of pulmonary artery remodeling in precision-cut lung slices upon ACLY inhibition. This study introduces a groundbreaking concept, linking disparate abnormalities in vascular diseases to a common pathogenetic denominator, ACLY. The identified “multiple hit” therapeutic approach presents potential targets for addressing complex vascular diseases, offering avenues for future clinical interventions.ONE SENTENCE SUMMARYOur study delineates the pivotal role of ATP-citrate lyase in orchestrating vascular remodeling, establishing it as a compelling translational target for therapeutic interventions in pulmonary hypertension and coronary artery disease.

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“…Cell viability was the percentage absorbance value of the treated cell relative to the untreated cell. Oil Red O, a fat-soluble dye that is usually used to stain neutral lipids, cholesterol esters and lipoproteins, has been long used for intracellular lipid staining and for tissue staining owing to its cellular permeability (Bharati et al, 2022;Du et al, 2023). Neutral lipids (mainly triglycerides) are stained with an orange-red tint, based on the higher solubility of Oil Red O in lipids compared with the dye solvents (Koopman et al, 2001).…”

Section: Cell Viability Assaymentioning

confidence: 99%

Response surface methodology optimization of extraction and enrichment conditions of total triterpenoid saponins from Celosiae Semen and evaluation of its lipid‐lowering activity

Yang,

Zhou,

Fan

et al. 2024

Biomedical Chromatography

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The saponin‐enriched extract from Celosiae Semen is a promising resource owing to its lipid‐lowering activity. However, triterpenoid saponins are difficult to extract owing to their high molecular weight and strong water solubility. The aim of this paper was to explore an eco‐friendly and effective technology of extraction and enrichment of total triterpenoid saponins to obtain high lipid‐lowering fractions. Initially, Box–Behnken design experiments were employed to optimize the heat reflux extraction process on the basic of mono‐factor experiments. Afterwards, the crude extract was further purified using D‐101 resin, and the purification parameters were investigated based on adsorption/desorption experiments and biological activity assay. Under optimal conditions, the purity of the finally obtained total triterpenoid saponins was increased by 7.28‐fold. The lipid‐lowering activities of the six main triterpenoid saponins were evaluated in HepG2 cells induced by palmitic acid. The results of Oil Red O staining showed that the compounds all exhibited potential lipid‐lowering activity. The structure–activity relationship analysis suggested that the oligosaccharide chain at C‐28 played an essential role in their lipid‐lowering activity and the substituent group at C‐23 site also showed important effects. The optimal extraction and purification methods may facilitate the utilization of Celosiae Semen for the industrial production as a functional food and drug.

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Oil Red O based method for exosome labelling and detection

Cited by 11 publications

References 15 publications

Prominin-1 null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy, and RPE atrophy

Prominin-1 null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy, and RPE atrophy

ATP Citrate Lyase Drives Vascular Remodeling Diseases Development Through Metabolic-Epigenetic Reprograming

Response surface methodology optimization of extraction and enrichment conditions of total triterpenoid saponins from Celosiae Semen and evaluation of its lipid‐lowering activity

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