Oil production at different stages of leaf development in Lippia alba

Abstract

Abstract:The aim of this work was to analyze terpene oil production and terpene synthases (TPS) gene expression from leaves at different developmental stages of different chemotypes of Lippia alba (Mill.) N.E. Br. ex Britton & P. Wilson, Verbenaceae. Hydro-distilled essential oil were used for chemical analysis and gene expression of three monoterpene synthase genes called LaTPS12, LaTPS23 and LaTPS25 were used for analyses of gene expression associated to oil production. The putative genes were associated to TPS-b gene class. Semi-quantitative PCR and quantitative PCR (qPCR) analysis were used to investigate the expression profile of those three putative genes in different leaf stages and different chemotypes. Additionally, total oil production and gene expression of putative TPS genes cloned from L. alba chemotype linalool were evaluated at different stages of leaf development. The expression level of those three genes was higher when the highest oil production was observed, mainly in young leaves at the fourth nodal segment for all evaluated chemotypes. Total oil production was higher at leaves that had unopened trichomes. We also observed that the 1mM of MeJA treatment increased the gene expression in all chemotypes after 24 h elicitation.

Chemical diversity and influence of plant age on the essential oil from Lippia sidoides Cham. germplasm

“…According to Matos and Innecco (2002), a proportional reduction occurs in the activity of metabolic pathways as the plant ages that reduces the synthesis of secondary metabolites. Pandeló et al (2012) studied the biosynthesis of L. alba essential oils based on the expression of three candidate genes (LATPS12, LATPS23 and LATPS25) that encode terpene synthases, and they evaluated different times of leaf development and observed that the maximum yield of essential oils in young leaves occurred at the fourth node compared with leaves at the second and eighth node. The aging of plant tissues is followed by a loss of vigor in physiological processes; therefore, an intense reduction can occur in the production of essential oils.…”

Quimiotipos, Extracción, Composición y Aplicaciones del Aceite Esencial de Lippia alba

“…Por otra parte, Hennebelle et al (2006) reportaron que tres quimiotipos de L. alba conservaron la composición del aceite esencial citral, linalol y carvona en diferentes condiciones ambientales o edafoclimáticas y sugirieron que las características genéticas, más que los factores ambientales, son las responsables de la composición del aceite esencial. Pandeló et al (2012) informaron que la producción del aceite esencial está relacionada con la expresión de tres genes de síntesis de terpeno putativos, clonados a partir del quimiotipo linalol de L. alba. Las plantas o quimiotipos de L. alba que son genéticamente estables y tienen baja influencia ambiental son los preferidos para el cultivo debido a la menor variación en los componentes químicos del aceite esencial.…”

Essential oil composition, antifungal activity and leaf anatomy of Lippia alba (Verbenaceae) from Brazilian Chaco

“…Essential oils from the leaves of L. alba have been categorized into different chemotypes, depending on their major constituents, such as linalool, citral, and carvone (Pandeló et al, 2012). Several biological properties of this plant, such as cytotoxicity, antioxidant, antibiofilm, anesthetic, antitumor, antibacterial, antifungal, antiinflammatory, antispasmodic activities and anxiolytic-like effects differ according to essential oil chemotype (Glamočlija et al, 2011;Trevisan et al, 2016;Tofiño-Rivera et al, 2016;Pandey et al, 2016, García et al, 2017.…”