Oil-In-Water Emulsion Droplets and Microfluidic Tools to Study B Cells Polarization and Mechanics of Immunological Synapse

Pinon, Léa; Pineau, Judith; Montel, Lorraine; Mesdjian, Olivier; Pierobon, Paolo; Fattaccioli, Jacques

doi:10.1016/j.bpj.2019.11.1805

Cited by 3 publications

(3 citation statements)

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“…Biological or biomedical applications of mechanical measurements are also very "wide-field" on the cellular / tissue formation level [13][14][15][16][17]. Such measurements are usually based on the elastic microfluidics, including elastomeric one (which can be applied not only for biomicrofluidic aims [18,19]).…”

Section: Introductionmentioning

confidence: 99%

Tensoresistor-Based Telemetric Strain-Gauge Lens-Less Detectors as Specialized Labs-on-a-Chip for Soil Mechanics and Foundation Monitoring

Orekhov¹,

Градов²

2022

Preprint

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<p>A current trend in the development of the applied measuring techniques is the design of integrated analytical systems on a chip, in particular, labs on a chip based on various physical principles of the substance analysis. Recently, the term "lab-on-a-chip" implied analytical microsystems, requiring macro-scopic readers for the data obtained, which strongly limited the possibilities of their introduction into the field studies and routine analytical measure-ments. However, a progress in the design of telemetric labs on a chip, in-cluding those capable of broadcasting the analytical signal to the receivers with positional sensitivity provided by the use of the labeled counting cham-bers, made it possible to use such devices beyond the scientific laboratories: in the field practice or in monitoring of the natural systems and artificial structures. This can be applied not only to the chemical and biological field measurements, but also to the mechanics, in particular, to geomechanics or soil mechanics. This work briefly considers the results obtained in the Rus-sian laboratory of the second author during the period up to 2011-2013, which have not been published earlier due to the know-how restrictions.</p>

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Section: Introductionmentioning

confidence: 99%

Tensoresistor-Based Telemetric Strain-Gauge Lens-Less Detectors as Specialized Labs-on-a-Chip for Soil Mechanics and Foundation Monitoring

Orekhov¹,

Градов²

2022

Preprint

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“…Among the last discoveries in medicine and biology research, Microfluidic Trap Arrays (MTAs) appear as very promising tools [19] for several applications like anticancer therapeutics [5,9] or the study of adhesion phenomena in immune synapse [17,20]. This success is due to the fact that MTAs allow an accurate spatio-temporal control of quite numerous and various isolated objects such as human [11] or vegetal [10] single cells, droplets [3,6,15,17,20] or polystyrene beads [17,18]. Moreover, because of fabrication simplicity, it is easy to combine several MTAs in parallel in a same microfluidic chip which is then called Microfluidic Cell Array (MCA), which increases the total number of isolated objects [1,13].…”

Section: Introductionmentioning

confidence: 99%

“…To tackle this issue, an alternative is to use much more complex geometries such as "U-shaped" [4] or "serpentine" [6,14,15] MCAs. Nevertheless, it is very difficult to have both a very large number of traps and a short trapping time with these complex geometries with respect to rectangular [17,20] or triangular [2] MTAs with their high trap density.…”

Section: Introductionmentioning

confidence: 99%

An in silico study to geometrically optimize microfluidic trap array for trapping efficiency

Ruyssen,

Fattaccioli,

Jullien

et al. 2021

Preprint

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Microfluidic Trap Arrays (MTAs) appear as very promising tools for several applications like cancer understanding and treatment or immune synapse research. The MTAs principle is the following: samples are diluted inside a fluid going through a microfluidic chamber composed of an array of a large number of traps. While following the fluid flow, samples can encounter traps that isolate them or very small group of them from the others. However, it often appears that many traps stay empty, even after a long time which can drastically reduce the number of exploitable samples. In this paper we built a Computational Fluid Dynamic (CFD) Finite Element Model with a specific streamlines analysis to investigate how the MTA geometry affects its trapping efficiency and how it is possible to optimize the MTA geometry in order to maximize the trapping efficiency. Our results shows that optimization of MTA trapping efficiency is a complex and non-intuitive process and is about finding a compromise between combinations of geometric parameters values that can not be achieved with a purely experimental approach. Streamlines analysis suggests that the important feature is to deviate the particle trajectories that can be done with: a diagonal pressure gradient, the use of low

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Tensoresistor-Based Microfluidics and Telemetric Strain-Gauge Lens-Less Detectors as Specialized Labs-on-a-Chip for Soil Mechanics and Foundation Monitoring

Orekhov

Градов

2021

Lecture Notes in Civil Engineering

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Oil-In-Water Emulsion Droplets and Microfluidic Tools to Study B Cells Polarization and Mechanics of Immunological Synapse

Cited by 3 publications

References 0 publications

Tensoresistor-Based Telemetric Strain-Gauge Lens-Less Detectors as Specialized Labs-on-a-Chip for Soil Mechanics and Foundation Monitoring

Tensoresistor-Based Telemetric Strain-Gauge Lens-Less Detectors as Specialized Labs-on-a-Chip for Soil Mechanics and Foundation Monitoring

An in silico study to geometrically optimize microfluidic trap array for trapping efficiency

Tensoresistor-Based Microfluidics and Telemetric Strain-Gauge Lens-Less Detectors as Specialized Labs-on-a-Chip for Soil Mechanics and Foundation Monitoring

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