Oil-free Microfluidic Spheroid Generation for Cancer Drug Testing
Jennifer Kieda
Abstract:<p>In this thesis, a droplet microfluidics platform for on-chip polymerization of all aqueous hydrogel multicellular spheroids (MCSs) is developed. First, dextran-alginate droplets containing MCF-7 breast cancer cells, surrounded by polyethylene glycol, are generated at a flow-focusing junction. Droplets travel to a second flow-focusing junction where they are introduced to calcium chloride and polymerize on-chip to form hydrogel MCSs. In drug-free experiments, hydrogels are incubated for six days, and c… Show more
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